PMID- 34681337 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20211026 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 10 IP - 10 DP - 2021 Sep 27 TI - Next Generation Sequencing of Single Nucleotide Polymorphic DNA-Markers in Selecting for Intramuscular Fat, Fat Melting Point, Omega-3 Long-Chain Polyunsaturated Fatty Acids and Meat Eating Quality in Tattykeel Australian White MARGRA Lamb. LID - 10.3390/foods10102288 [doi] LID - 2288 AB - Meat quality data can only be obtained after slaughter when selection decisions about the live animal are already too late. Carcass estimated breeding values present major precision problems due to low accuracy, and by the time an informed decision on the genetic merit for meat quality is made, the animal is already dead. We report for the first time, a targeted next-generation sequencing (NGS) of single nucleotide polymorphisms (SNP) of lipid metabolism genes in Tattykeel Australian White (TAW) sheep of the MARGRA lamb brand, utilizing an innovative and minimally invasive muscle biopsy sampling technique for directly quantifying the genetic worth of live lambs for health-beneficial omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA), intramuscular fat (IMF), and fat melting point (FMP). NGS of stearoyl-CoA desaturase (SCD), fatty acid binding protein-4 (FABP4), and fatty acid synthase (FASN) genes identified functional SNP with unique DNA marker signatures for TAW genetics. The SCD g.23881050T>C locus was significantly associated with IMF, C22:6n-3, and C22:5n-3; FASN g.12323864A>G locus with FMP, C18:3n-3, C18:1n-9, C18:0, C16:0, MUFA, and FABP4 g.62829478A>T locus with IMF. These add new knowledge, precision, and reliability in directly making early and informed decisions on live sheep selection and breeding for health-beneficial n-3 LC-PUFA, FMP, IMF and superior meat-eating quality at the farmgate level. The findings provide evidence that significant associations exist between SNP of lipid metabolism genes and n-3 LC-PUFA, IMF, and FMP, thus underpinning potential marker-assisted selection for meat-eating quality traits in TAW lambs. FAU - Pewan, Shedrach Benjamin AU - Pewan SB AUID- ORCID: 0000-0002-7254-0917 AD - Animal Genetics and Nutrition, Veterinary Sciences Discipline, College of Public Health, Medical and Veterinary Sciences, Division of Tropical Health and Medicine, James Cook University, Townsville, QLD 4811, Australia. AD - National Veterinary Research Institute, Private Mail Bag 01 Vom, Plateau State, Nigeria. FAU - Otto, John Roger AU - Otto JR AUID- ORCID: 0000-0002-6578-0787 AD - Animal Genetics and Nutrition, Veterinary Sciences Discipline, College of Public Health, Medical and Veterinary Sciences, Division of Tropical Health and Medicine, James Cook University, Townsville, QLD 4811, Australia. FAU - Huerlimann, Roger AU - Huerlimann R AUID- ORCID: 0000-0002-6020-334X AD - Marine Climate Change Unit, Okinawa Institute of Science and Technology, 1919-1 Tancha, Onna-son, Okinawa 904-0495, Japan. AD - Centre for Sustainable Tropical Fisheries and Aquaculture and Centre for Tropical Bioinformatics and Molecular Biology, College of Science and Engineering, James Cook University, Townsville, QLD 4811, Australia. FAU - Budd, Alyssa Maree AU - Budd AM AD - Centre for Sustainable Tropical Fisheries and Aquaculture and Centre for Tropical Bioinformatics and Molecular Biology, College of Science and Engineering, James Cook University, Townsville, QLD 4811, Australia. FAU - Mwangi, Felista Waithira AU - Mwangi FW AD - Animal Genetics and Nutrition, Veterinary Sciences Discipline, College of Public Health, Medical and Veterinary Sciences, Division of Tropical Health and Medicine, James Cook University, Townsville, QLD 4811, Australia. FAU - Edmunds, Richard Crawford AU - Edmunds RC AD - Animal Genetics and Nutrition, Veterinary Sciences Discipline, College of Public Health, Medical and Veterinary Sciences, Division of Tropical Health and Medicine, James Cook University, Townsville, QLD 4811, Australia. FAU - Holman, Benjamin William Behrens AU - Holman BWB AUID- ORCID: 0000-0002-8458-4511 AD - Centre for Red Meat and Sheep Development, NSW Department of Primary Industries, Cowra, NSW 2794, Australia. FAU - Henry, Michelle Lauren Elizabeth AU - Henry MLE AD - Gundagai Meat Processors, 2916 Gocup Road, South Gundagai, NSW 2722, Australia. AD - Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Melbourne, VIC 3010, Australia. FAU - Kinobe, Robert Tumwesigye AU - Kinobe RT AUID- ORCID: 0000-0003-1166-0947 AD - Animal Genetics and Nutrition, Veterinary Sciences Discipline, College of Public Health, Medical and Veterinary Sciences, Division of Tropical Health and Medicine, James Cook University, Townsville, QLD 4811, Australia. FAU - Adegboye, Oyelola Abdulwasiu AU - Adegboye OA AUID- ORCID: 0000-0002-9793-8024 AD - Public Health and Tropical Medicine Discipline, College of Public Health, Medical and Veterinary Sciences, Division of Tropical Health and Medicine, James Cook University, Townsville, QLD 4811, Australia. FAU - Malau-Aduli, Aduli Enoch Othniel AU - Malau-Aduli AEO AUID- ORCID: 0000-0002-3660-7862 AD - Animal Genetics and Nutrition, Veterinary Sciences Discipline, College of Public Health, Medical and Veterinary Sciences, Division of Tropical Health and Medicine, James Cook University, Townsville, QLD 4811, Australia. LA - eng GR - Innovations Connections Grant/Department of Industry, Innovation and Science, Australian Government/ GR - Science Industry Endowment Fund STEM Business Industrial Research Fellowship/Commonwealth Scientific and Industrial Research Organisation/ PT - Journal Article DEP - 20210927 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC8535056 OTO - NOTNLM OT - FABP4 OT - FASN OT - FMP OT - IMF OT - SCD OT - SNP OT - TAW MARGRA lamb OT - biopsy OT - eating quality OT - n-3 LC-PUFA COIS- The authors declare no conflict of interest. The funders had no role in the design of the study; collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. EDAT- 2021/10/24 06:00 MHDA- 2021/10/24 06:01 PMCR- 2021/09/27 CRDT- 2021/10/23 01:07 PHST- 2021/08/27 00:00 [received] PHST- 2021/09/13 00:00 [revised] PHST- 2021/09/17 00:00 [accepted] PHST- 2021/10/23 01:07 [entrez] PHST- 2021/10/24 06:00 [pubmed] PHST- 2021/10/24 06:01 [medline] PHST- 2021/09/27 00:00 [pmc-release] AID - foods10102288 [pii] AID - foods-10-02288 [pii] AID - 10.3390/foods10102288 [doi] PST - epublish SO - Foods. 2021 Sep 27;10(10):2288. doi: 10.3390/foods10102288.