PMID- 34681868
OWN - NLM
STAT- MEDLINE
DCOM- 20211216
LR  - 20211216
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 22
IP  - 20
DP  - 2021 Oct 18
TI  - Molecular Cytogenetic Analysis of the Introgression between Agropyron cristatum P 
      Genome and Wheat Genome.
LID - 10.3390/ijms222011208 [doi]
LID - 11208
AB  - Agropyron cristatum (2n = 4x = 28, PPPP) is an important wild relative of common 
      wheat (Triticum aestivum L., 2n = 6x = 42). A previous report showed that the 
      wheat-A. cristatum 6P translocation line WAT655 carrying A. cristatum 6PS 
      (0.81-1.00) exhibited high resistance to prevalent physiological races of stripe 
      rust (CYR32 and CYR33). In this study, three disease resistance-related 
      transcripts, which were mapped to A. cristatum 6PS (0.81-1.00) through the 
      analysis of specific molecular markers, were acquired from among A. cristatum 
      full-length transcripts. The BC(5)F(2) and BC(5)F(2:3) genetic populations of the 
      translocation line WAT655 were analyzed by using three disease resistance-related 
      gene markers, A. cristatum P genome-specific markers, and fluorescence in situ 
      hybridization (FISH). The results revealed that the introgression between A. 
      cristatum P genome and wheat genome was observed in progenies of the genetic 
      populations of the translocation line WAT655 and the physical positions of the 
      three genes were considerably adjacent on A. cristatum 6PS (0.81-1.00) according 
      to the FISH results. Additionally, kompetitive allele-specific PCR (KASP) markers 
      of the three genes were developed to detect and acquire 24 breeding lines 
      selected from the progenies of the distant hybridization of wheat and A. 
      cristatum, which showed resistance to physiological races of stripe rust (CYR32 
      and CYR33) and other desirable agronomic traits according to the field 
      investigation. In conclusion, this study not only provides new insights into the 
      introgression between A. cristatum P genome and wheat genome but also provides 
      the desirable germplasms for breeding practice.
FAU - Zhang, Zhi
AU  - Zhang Z
AUID- ORCID: 0000-0002-3684-7882
AD  - National Key Facility for Crop Gene Resources and Genetic Improvement, Institute 
      of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, 
      China.
FAU - Zhou, Shenghui
AU  - Zhou S
AD  - National Key Facility for Crop Gene Resources and Genetic Improvement, Institute 
      of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, 
      China.
FAU - Liu, Weihua
AU  - Liu W
AD  - National Key Facility for Crop Gene Resources and Genetic Improvement, Institute 
      of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, 
      China.
FAU - Song, Liqiang
AU  - Song L
AD  - Center for Agricultural Resources Research, Institute of Genetics and 
      Developmental Biology of Sciences, Shijiazhuang 050022, China.
FAU - Zhang, Jinpeng
AU  - Zhang J
AD  - National Key Facility for Crop Gene Resources and Genetic Improvement, Institute 
      of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, 
      China.
FAU - Han, Haiming
AU  - Han H
AD  - National Key Facility for Crop Gene Resources and Genetic Improvement, Institute 
      of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, 
      China.
FAU - Yang, Xinming
AU  - Yang X
AD  - National Key Facility for Crop Gene Resources and Genetic Improvement, Institute 
      of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, 
      China.
FAU - Lin, Yida
AU  - Lin Y
AD  - National Key Facility for Crop Gene Resources and Genetic Improvement, Institute 
      of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, 
      China.
FAU - Li, Xiuquan
AU  - Li X
AD  - National Key Facility for Crop Gene Resources and Genetic Improvement, Institute 
      of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, 
      China.
FAU - Li, Lihui
AU  - Li L
AD  - National Key Facility for Crop Gene Resources and Genetic Improvement, Institute 
      of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, 
      China.
LA  - eng
GR  - CAAS-ASTIP-2021-ICS/Chinese Academy of Agricultural Sciences/
GR  - 31801359/National Science Foundation of China/
PT  - Journal Article
DEP - 20211018
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Genetic Markers)
RN  - Puccinia striiformis
SB  - IM
MH  - Agropyron/*genetics
MH  - Chromosome Mapping
MH  - Chromosomes, Artificial, Bacterial
MH  - Chromosomes, Plant
MH  - Cytogenetic Analysis
MH  - Disease Resistance/*genetics
MH  - Genetic Introgression/*genetics
MH  - Genetic Markers
MH  - *Genome, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - Plant Breeding
MH  - Plant Diseases/genetics/microbiology
MH  - Puccinia/pathogenicity
MH  - Triticum/*genetics
PMC - PMC8539888
OTO - NOTNLM
OT  - Agropyron cristatum
OT  - common wheat
OT  - introgression
OT  - stripe rust
OT  - translocation line
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2021/10/24 06:00
MHDA- 2021/12/17 06:00
PMCR- 2021/10/18
CRDT- 2021/10/23 01:09
PHST- 2021/09/21 00:00 [received]
PHST- 2021/10/14 00:00 [revised]
PHST- 2021/10/15 00:00 [accepted]
PHST- 2021/10/23 01:09 [entrez]
PHST- 2021/10/24 06:00 [pubmed]
PHST- 2021/12/17 06:00 [medline]
PHST- 2021/10/18 00:00 [pmc-release]
AID - ijms222011208 [pii]
AID - ijms-22-11208 [pii]
AID - 10.3390/ijms222011208 [doi]
PST - epublish
SO  - Int J Mol Sci. 2021 Oct 18;22(20):11208. doi: 10.3390/ijms222011208.