PMID- 34684414
OWN - NLM
STAT- MEDLINE
DCOM- 20211129
LR  - 20211129
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 13
IP  - 10
DP  - 2021 Sep 28
TI  - Antioxidants Supplementation Reduces Ceramide Synthesis Improving the Cardiac 
      Insulin Transduction Pathway in a Rodent Model of Obesity.
LID - 10.3390/nu13103413 [doi]
LID - 3413
AB  - Obesity-related disruption in lipid metabolism contributes to cardiovascular 
      dysfunction. Despite numerous studies on lipid metabolism in the left ventricle, 
      there is no data describing the influence of n-acetylcysteine (NAC) and alpha-lipoic 
      acid (ALA), as glutathione precursors, on sphingolipid metabolism, and insulin 
      resistance (IR) occurrence. The aim of our experiment was to evaluate the 
      influence of chronic antioxidants administration on myocardial sphingolipid state 
      and intracellular insulin signaling as a potential therapeutic strategy for 
      obesity-related cardiovascular IR. The experiment was conducted on male Wistar 
      rats fed a standard rodent chow or a high-fat diet with intragastric 
      administration of NAC or ALA for eight weeks. Cardiac and plasma sphingolipid 
      species were assessed by high-performance liquid chromatography (HPLC). The 
      proteins expressed from sphingolipid and insulin signaling pathways were 
      determined by Western blot. Antioxidant supplementation markedly reduced ceramide 
      accumulation by lowering the expression of selected proteins from the 
      sphingolipid pathway and simultaneously increased the myocardial 
      sphingosine-1-phosphate level. Moreover, NAC and ALA augmented the expression of 
      GLUT4 and the phosphorylation state of Akt (Ser473) and GSK3beta (Ser9), which 
      improved the intracellular insulin transduction pathway. Based on our results, we 
      may postulate that NAC and ALA have a beneficial influence on the cardiac 
      ceramidose under IR conditions.
FAU - Hodun, Katarzyna
AU  - Hodun K
AUID- ORCID: 0000-0002-2102-7638
AD  - Department of Physiology, Medical University of Bialystok, 15-089 Bialystok, 
      Poland.
FAU - Sztolsztener, Klaudia
AU  - Sztolsztener K
AUID- ORCID: 0000-0003-1354-7585
AD  - Department of Physiology, Medical University of Bialystok, 15-089 Bialystok, 
      Poland.
FAU - Chabowski, Adrian
AU  - Chabowski A
AUID- ORCID: 0000-0002-7407-8156
AD  - Department of Physiology, Medical University of Bialystok, 15-089 Bialystok, 
      Poland.
LA  - eng
GR  - SUB/1/DN/20/004/1118/Uniwersytet Medyczny w Bialymstoku/
PT  - Journal Article
DEP - 20210928
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 0 (Antioxidants)
RN  - 0 (Biomarkers)
RN  - 0 (Ceramides)
RN  - 0 (Insulin)
RN  - 0 (Sphingolipids)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animal Feed
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Biomarkers/blood/metabolism
MH  - Body Weight
MH  - Ceramides/*biosynthesis
MH  - Diet, High-Fat
MH  - *Dietary Supplements
MH  - Glucose/metabolism
MH  - Insulin/*metabolism
MH  - Insulin Resistance
MH  - Male
MH  - Metabolic Networks and Pathways
MH  - Models, Animal
MH  - Myocardium/*metabolism
MH  - Obesity/etiology/*metabolism
MH  - Phosphorylation
MH  - Rats
MH  - Rodentia
MH  - Signal Transduction/*drug effects
MH  - Sphingolipids/metabolism
PMC - PMC8541644
OTO - NOTNLM
OT  - cardiovascular diseases
OT  - ceramide
OT  - insulin resistance
OT  - left ventricle
OT  - n-acetylcysteine
OT  - sphingolipid
OT  - alpha-lipoic acid
COIS- The authors declare no conflict of interest.
EDAT- 2021/10/24 06:00
MHDA- 2021/11/30 06:00
PMCR- 2021/09/28
CRDT- 2021/10/23 01:18
PHST- 2021/08/27 00:00 [received]
PHST- 2021/09/24 00:00 [revised]
PHST- 2021/09/24 00:00 [accepted]
PHST- 2021/10/23 01:18 [entrez]
PHST- 2021/10/24 06:00 [pubmed]
PHST- 2021/11/30 06:00 [medline]
PHST- 2021/09/28 00:00 [pmc-release]
AID - nu13103413 [pii]
AID - nutrients-13-03413 [pii]
AID - 10.3390/nu13103413 [doi]
PST - epublish
SO  - Nutrients. 2021 Sep 28;13(10):3413. doi: 10.3390/nu13103413.