PMID- 34685547
OWN - NLM
STAT- MEDLINE
DCOM- 20220105
LR  - 20220105
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 10
IP  - 10
DP  - 2021 Sep 28
TI  - Zonula occludens 2 and Cell-Cell Contacts Are Required for Normal Nuclear Shape 
      in Epithelia.
LID - 10.3390/cells10102568 [doi]
LID - 2568
AB  - MAGUK protein ZO-2 is present at tight junctions (TJs) and nuclei. In MDCK ZO-2 
      knockdown (KD) cells, nuclei exhibit an irregular shape with lobules and 
      indentations. This condition correlates with an increase in DNA double strand 
      breaks, however cells are not senescent and instead become resistant to 
      UV-induced senescence. The irregular nuclear shape is also observed in isolated 
      cells and in those without TJs, due to the lack of extracellular calcium. The 
      aberrant nuclear shape of ZO-2 KD cells is not accompanied by a reduced 
      expression of lamins A/C and B and lamin B receptors. Instead, it involves a 
      decrease in constitutive and facultative heterochromatin, and microtubule 
      instability that is restored with docetaxel. ZO-2 KD cells over-express SUN-1 
      that crosses the inner nuclear membrane and connects the nucleoskeleton of lamin 
      A to nesprins, which traverse the outer nuclear membrane. Nesprins-3 and -4 that 
      indirectly bind on their cytoplasmic face to vimentin and microtubules, 
      respectively, are also over-expressed in ZO-2 KD cells, whereas vimentin is 
      depleted. SUN-1 and lamin B1 co-immunoprecipitate with ZO-2, and SUN-1 associates 
      to ZO-2 in a pull-down assay. Our results suggest that ZO-2 forms a complex with 
      SUN-1 and lamin B1 at the inner nuclear membrane, and that ZO-2 and cell-cell 
      contacts are required for a normal nuclear shape.
FAU - Hernandez-Guzman, Christian
AU  - Hernandez-Guzman C
AUID- ORCID: 0000-0003-4372-2073
AD  - Center for Research and Advanced Studies (Cinvestav), Department of Physiology, 
      Biophysics and Neuroscience, Ave IPN 2508, Mexico City 07360, Mexico.
FAU - Gallego-Gutierrez, Helios
AU  - Gallego-Gutierrez H
AUID- ORCID: 0000-0003-0369-9083
AD  - Center for Research and Advanced Studies (Cinvestav), Department of Physiology, 
      Biophysics and Neuroscience, Ave IPN 2508, Mexico City 07360, Mexico.
FAU - Chavez-Munguia, Bibiana
AU  - Chavez-Munguia B
AD  - Center for Research and Advanced Studies (Cinvestav), Department of Infectomics 
      and Molecular Pathogenesis, Ave IPN 2508, Mexico City 07360, Mexico.
FAU - Martin-Tapia, Dolores
AU  - Martin-Tapia D
AD  - Center for Research and Advanced Studies (Cinvestav), Department of Physiology, 
      Biophysics and Neuroscience, Ave IPN 2508, Mexico City 07360, Mexico.
FAU - Gonzalez-Mariscal, Lorenza
AU  - Gonzalez-Mariscal L
AD  - Center for Research and Advanced Studies (Cinvestav), Department of Physiology, 
      Biophysics and Neuroscience, Ave IPN 2508, Mexico City 07360, Mexico.
LA  - eng
GR  - FIDSC2018/33/SEP-Cinvestav/
GR  - FORDECYT-PRONACES-140644/Consejo Nacional de Ciencia y Tecnologia/
GR  - 407499/Consejo Nacional de Ciencia y Tecnologia/
GR  - 282075/Consejo Nacional de Ciencia y Tecnologia/
GR  - 2018AD0009-11/Consejo Mexiquense de Ciencia y Tecnologia/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210928
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - 0 (Zonula Occludens-2 Protein)
SB  - IM
MH  - Cell Communication/*immunology
MH  - Epithelium/*metabolism
MH  - Humans
MH  - Transfection
MH  - Zonula Occludens-2 Protein/*metabolism
PMC - PMC8534263
OTO - NOTNLM
OT  - LINC
OT  - SUN-1
OT  - ZO-2
OT  - nuclear shape
OT  - senescence
OT  - vimentin
COIS- The authors declare no conflict of interest.
EDAT- 2021/10/24 06:00
MHDA- 2022/01/06 06:00
PMCR- 2021/09/28
CRDT- 2021/10/23 01:22
PHST- 2021/08/20 00:00 [received]
PHST- 2021/09/04 00:00 [revised]
PHST- 2021/09/06 00:00 [accepted]
PHST- 2021/10/23 01:22 [entrez]
PHST- 2021/10/24 06:00 [pubmed]
PHST- 2022/01/06 06:00 [medline]
PHST- 2021/09/28 00:00 [pmc-release]
AID - cells10102568 [pii]
AID - cells-10-02568 [pii]
AID - 10.3390/cells10102568 [doi]
PST - epublish
SO  - Cells. 2021 Sep 28;10(10):2568. doi: 10.3390/cells10102568.