PMID- 34686744
OWN - NLM
STAT- MEDLINE
DCOM- 20220121
LR  - 20220121
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Oct 22
TI  - Assessment on the oil accumulation by knockdown of triacylglycerol lipase in the 
      oleaginous diatom Fistulifera solaris.
PG  - 20905
LID - 10.1038/s41598-021-00453-w [doi]
LID - 20905
AB  - Microalgae are promising producers of biofuel due to higher accumulation of 
      triacylglycerol (TAG). However, further improvement of the lipid metabolism is 
      critical for feasible application of microalgae in industrial production of 
      biofuel. Suppression of lipid degradation pathways is a promising way to 
      remarkably increase the lipid production in model diatoms. In this study, we 
      established an antisense-based knockdown (KD) technique in the marine oleaginous 
      diatom, Fistulifera solaris. This species has a capability to accumulate high 
      content of lipids. Tgl1 KD showed positive impact on cell growth and lipid 
      accumulation in conventional culture in f/2 medium, resulting in higher oil 
      contents compared to wild type strain. However, these impacts of Tgl1 KD were 
      slight when the cells were subjected to the two-stage growth system. The Tgl1 KD 
      resulted in slight change of fatty acid composition; increasing in C14:0, C16:0 
      and C16:1, and decreasing in C20:5. This study indicates that, although Tgl1 
      played a certain role in lipid degradation in F. solaris, suppression of only a 
      single type of TAG lipase was not significantly effective to improve the lipid 
      production. Comprehensive understanding of the lipid catabolism in this microalga 
      is essential to further improve the lipid production.
CI  - (c) 2021. The Author(s).
FAU - Maeda, Yoshiaki
AU  - Maeda Y
AD  - Division of Biotechnology and Life Science, Institute of Engineering, Tokyo 
      University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 
      184-8588, Japan.
FAU - Watanabe, Kahori
AU  - Watanabe K
AD  - Division of Biotechnology and Life Science, Institute of Engineering, Tokyo 
      University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 
      184-8588, Japan.
FAU - Kaha, Marshila
AU  - Kaha M
AD  - Division of Biotechnology and Life Science, Institute of Engineering, Tokyo 
      University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 
      184-8588, Japan.
FAU - Yabu, Yusuke
AU  - Yabu Y
AD  - Division of Biotechnology and Life Science, Institute of Engineering, Tokyo 
      University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 
      184-8588, Japan.
FAU - Yoshino, Tomoko
AU  - Yoshino T
AD  - Division of Biotechnology and Life Science, Institute of Engineering, Tokyo 
      University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 
      184-8588, Japan.
FAU - Matsumoto, Mitsufumi
AU  - Matsumoto M
AD  - Biotechnology Laboratory, Electric Power Development CO., Ltd., 1, 
      Yanagisaki-machi, Wakamatsu-ku, Kitakyushu, Fukuoka, 808-0111, Japan.
FAU - Tanaka, Tsuyoshi
AU  - Tanaka T
AD  - Division of Biotechnology and Life Science, Institute of Engineering, Tokyo 
      University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 
      184-8588, Japan. tsuyo@cc.tuat.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211022
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Biofuels)
RN  - 0 (Fatty Acids)
RN  - 0 (Oils)
RN  - 0 (Triglycerides)
RN  - EC 3.1.1.3 (Lipase)
SB  - IM
MH  - Biofuels
MH  - Diatoms/*metabolism
MH  - Fatty Acids/metabolism
MH  - Lipase/*metabolism
MH  - Lipid Metabolism/physiology
MH  - Microalgae/metabolism
MH  - Oils/*metabolism
MH  - Triglycerides/*metabolism
PMC - PMC8536745
COIS- The authors declare no competing interests.
EDAT- 2021/10/24 06:00
MHDA- 2022/01/22 06:00
PMCR- 2021/10/22
CRDT- 2021/10/23 06:06
PHST- 2021/07/30 00:00 [received]
PHST- 2021/10/06 00:00 [accepted]
PHST- 2021/10/23 06:06 [entrez]
PHST- 2021/10/24 06:00 [pubmed]
PHST- 2022/01/22 06:00 [medline]
PHST- 2021/10/22 00:00 [pmc-release]
AID - 10.1038/s41598-021-00453-w [pii]
AID - 453 [pii]
AID - 10.1038/s41598-021-00453-w [doi]
PST - epublish
SO  - Sci Rep. 2021 Oct 22;11(1):20905. doi: 10.1038/s41598-021-00453-w.