PMID- 34689292
OWN - NLM
STAT- MEDLINE
DCOM- 20220204
LR  - 20220204
IS  - 2523-899X (Electronic)
IS  - 2523-899X (Linking)
VI  - 41
IP  - 5
DP  - 2021 Oct
TI  - Protective Effect of Human Umbilical Cord Mesenchymal Stem Cells in 
      Glucocorticoid-induced Osteonecrosis of Femoral Head.
PG  - 909-915
LID - 10.1007/s11596-021-2439-6 [doi]
AB  - OBJECTIVE: To evaluate the effect of human umbilical cord mesenchymal stem cells 
      (hUC-MSCs) on preventing rats from glucocorticoid-induced osteonecrosis of 
      femoral head (GCONFH) in the early stage in vivo and to investigate the possible 
      mechanism of hUC-MSCs in regulating the balance of osteogenesis and adipogenesis. 
      METHODS: All rats were randomly divided into 3 groups: control group (C group), 
      model group (M group), and intervention group (I group). The model of GC-ONFH was 
      developed by a sequential administration of lipopolysaccharide and 
      methylprednisolone. The rats in the I group were treated with caudal vein 
      injection of hUC-MSCs. Six weeks later, the blood samples were obtained to 
      measure the activity of alkaline phosphatase (ALP) and the content of 
      triglyceride (TG) in serum, and the femoral heads were harvested and observed by 
      hematoxylin-eosin staining, Micro-CT, Western blot and real-time quantitative 
      polymerase chain reaction. RESULTS: After intervention of hUC-MSCs, the necrosis 
      rate of femoral head decreased from 83% (10/12) to 33% (4/12), the rate of empty 
      bone lacuna was significantly decreased, the activity of ALP increased 
      significantly, the content of TG decreased significantly, the bone density 
      increased obviously, the expression of RUNX2 and Col I increased significantly 
      and the expression of PPARgamma decreased significantly. CONCLUSION: These results 
      revealed that caudal vein injection of hUC-MSCs can effectively reduce the 
      incidence of GC-ONFH in rats by increasing ALP activity and reducing TG content 
      in serum, increasing bone mineral density, promoting the expression of RUNX2 and 
      Col I, and inhibiting the expression of PPARgamma.
CI  - (c) 2021. Huazhong University of Science and Technology.
FAU - Qiu, Chen
AU  - Qiu C
AD  - Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, 430060, 
      China.
AD  - Department of Orthopedics, Xiangyang No. 1 People's Hospital, Hubei University of 
      Medicine, Xiangyang, 443000, China.
FAU - Zhou, Jian-Lin
AU  - Zhou JL
AD  - Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, 430060, 
      China.
FAU - Deng, Shuang
AU  - Deng S
AD  - Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, 430060, 
      China.
FAU - Long, Lin-Sheng
AU  - Long LS
AD  - Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, 430060, 
      China.
FAU - Peng, Hao
AU  - Peng H
AD  - Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, 430060, 
      China. penghao8599@163.com.
LA  - eng
PT  - Journal Article
DEP - 20211023
PL  - China
TA  - Curr Med Sci
JT  - Current medical science
JID - 101729993
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (Glucocorticoids)
RN  - 0 (PPAR gamma)
RN  - 0 (Runx2 protein, rat)
RN  - 0 (Triglycerides)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Core Binding Factor Alpha 1 Subunit/genetics/metabolism
MH  - Femur Head Necrosis/chemically induced/metabolism/pathology/*therapy
MH  - Glucocorticoids/*adverse effects
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/cytology
MH  - PPAR gamma/genetics/metabolism
MH  - Random Allocation
MH  - Rats
MH  - Treatment Outcome
MH  - Triglycerides/blood
MH  - Umbilical Cord/*cytology
MH  - Up-Regulation
OTO - NOTNLM
OT  - Col I
OT  - PPARgamma
OT  - RUNX2
OT  - glucocorticoid-induced osteonecrosis of femoral head
OT  - human umbilical cord mesenchymal stem cell
EDAT- 2021/10/25 06:00
MHDA- 2022/02/05 06:00
CRDT- 2021/10/24 20:56
PHST- 2020/12/20 00:00 [received]
PHST- 2021/04/15 00:00 [accepted]
PHST- 2021/10/25 06:00 [pubmed]
PHST- 2022/02/05 06:00 [medline]
PHST- 2021/10/24 20:56 [entrez]
AID - 10.1007/s11596-021-2439-6 [pii]
AID - 10.1007/s11596-021-2439-6 [doi]
PST - ppublish
SO  - Curr Med Sci. 2021 Oct;41(5):909-915. doi: 10.1007/s11596-021-2439-6. Epub 2021 
      Oct 23.