PMID- 34689550
OWN - NLM
STAT- MEDLINE
DCOM- 20211104
LR  - 20211104
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 69
IP  - 43
DP  - 2021 Nov 3
TI  - Linear Epitopes Play an Important Role in the Immunoglobulin G 
      (IgG)/Immunoglobulin E (IgE)-Binding Capacity of Scy p 4.
PG  - 12870-12879
LID - 10.1021/acs.jafc.1c05464 [doi]
AB  - Sarcoplasmic calcium-binding protein is a stable allergen in Scylla paramamosain 
      and named Scy p 4. To explore the importance of linear epitopes in the 
      immunoglobulin G (IgG)/immunoglobulin E (IgE)-binding capacity of Scy p 4, 
      chemical denaturants were used to destroy the structure. Scy p 4 was reduced with 
      dithiothreitol and subsequently alkylated with iodoacetamide (IAA). Furthermore, 
      the structural analysis indicated that IAA-Scy p 4 was an unstructured protein. 
      The inhibition enzyme-linked immunosorbent assay showed that IAA-Scy p 4 could 
      inhibit the binding of Scy p 4 to sensitize serum, with inhibition rates reached 
      55%. Moreover, the linear mimotopes of Scy p 4 were predicted in silico. Three 
      linear epitopes were verified by serological tests and named L-Scy p 4-1 
      (AA(76-91)), L-Scy p 4-2 (AA(111-125)), and L-Scy p 4-3 (AA(137-146)). Overall, 
      these data provide an understanding of the relationship between the structure and 
      allergenicity about Scy p 4, and the identified linear epitopes can be used for 
      diagnosis and food processing of shellfish allergy.
FAU - Chen, Yi-Yu
AU  - Chen YY
AD  - College of Food and Biological Engineering, Xiamen Key Laboratory of Marine 
      Functional Food, Fujian Provincial Engineering Technology Research Center of 
      Marine Functional Food, Fujian Collaborative Innovation Center for Exploitation 
      and Utilization of Marine Biological Resources, Jimei University, Xiamen, Fujian 
      361021, People's Republic of China.
FAU - Li, Meng-Si
AU  - Li MS
AD  - College of Food and Biological Engineering, Xiamen Key Laboratory of Marine 
      Functional Food, Fujian Provincial Engineering Technology Research Center of 
      Marine Functional Food, Fujian Collaborative Innovation Center for Exploitation 
      and Utilization of Marine Biological Resources, Jimei University, Xiamen, Fujian 
      361021, People's Republic of China.
FAU - Liu, Meng
AU  - Liu M
AD  - College of Marine Biology, Xiamen Ocean Vocational College, Xiamen, Fujian 
      361102, People's Republic of China.
FAU - Yun, Xiao
AU  - Yun X
AD  - College of Food and Biological Engineering, Xiamen Key Laboratory of Marine 
      Functional Food, Fujian Provincial Engineering Technology Research Center of 
      Marine Functional Food, Fujian Collaborative Innovation Center for Exploitation 
      and Utilization of Marine Biological Resources, Jimei University, Xiamen, Fujian 
      361021, People's Republic of China.
FAU - Huan, Fei
AU  - Huan F
AD  - College of Food and Biological Engineering, Xiamen Key Laboratory of Marine 
      Functional Food, Fujian Provincial Engineering Technology Research Center of 
      Marine Functional Food, Fujian Collaborative Innovation Center for Exploitation 
      and Utilization of Marine Biological Resources, Jimei University, Xiamen, Fujian 
      361021, People's Republic of China.
FAU - Liu, Qing-Mei
AU  - Liu QM
AD  - College of Food and Biological Engineering, Xiamen Key Laboratory of Marine 
      Functional Food, Fujian Provincial Engineering Technology Research Center of 
      Marine Functional Food, Fujian Collaborative Innovation Center for Exploitation 
      and Utilization of Marine Biological Resources, Jimei University, Xiamen, Fujian 
      361021, People's Republic of China.
FAU - Cao, Min-Jie
AU  - Cao MJ
AD  - College of Food and Biological Engineering, Xiamen Key Laboratory of Marine 
      Functional Food, Fujian Provincial Engineering Technology Research Center of 
      Marine Functional Food, Fujian Collaborative Innovation Center for Exploitation 
      and Utilization of Marine Biological Resources, Jimei University, Xiamen, Fujian 
      361021, People's Republic of China.
FAU - Chen, Gui-Xia
AU  - Chen GX
AD  - Women and Children's Hospital Affiliated to Xiamen University, Xiamen, Fujian 
      361003, People's Republic of China.
FAU - Lai, Dong
AU  - Lai D
AD  - The Second Affiliated Hospital of Xiamen Medical College, Xiamen, Fujian 361021, 
      People's Republic of China.
FAU - Liu, Guang-Ming
AU  - Liu GM
AUID- ORCID: 0000-0002-8689-0504
AD  - College of Food and Biological Engineering, Xiamen Key Laboratory of Marine 
      Functional Food, Fujian Provincial Engineering Technology Research Center of 
      Marine Functional Food, Fujian Collaborative Innovation Center for Exploitation 
      and Utilization of Marine Biological Resources, Jimei University, Xiamen, Fujian 
      361021, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20211024
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Allergens)
RN  - 0 (Epitopes)
RN  - 0 (Immunoglobulin G)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens
MH  - Epitopes
MH  - Humans
MH  - Immunoglobulin E
MH  - *Immunoglobulin G
MH  - *Shellfish Hypersensitivity
OTO - NOTNLM
OT  - IgG/IgE-binding capacity
OT  - denaturation treatment
OT  - disulfide bonds
OT  - linear epitopes
OT  - sarcoplasmic calcium-binding protein
EDAT- 2021/10/26 06:00
MHDA- 2021/11/05 06:00
CRDT- 2021/10/25 05:29
PHST- 2021/10/26 06:00 [pubmed]
PHST- 2021/11/05 06:00 [medline]
PHST- 2021/10/25 05:29 [entrez]
AID - 10.1021/acs.jafc.1c05464 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2021 Nov 3;69(43):12870-12879. doi: 10.1021/acs.jafc.1c05464. 
      Epub 2021 Oct 24.