PMID- 34690454
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221003
IS  - 0971-4502 (Print)
IS  - 0974-0449 (Electronic)
IS  - 0971-4502 (Linking)
VI  - 37
IP  - 4
DP  - 2021 Oct
TI  - A Systematic Cytogenetic Strategy to Identify Masked Hypodiploidy in Precursor B 
      Acute Lymphoblastic Leukemia in Low Resource Settings.
PG  - 576-585
LID - 10.1007/s12288-021-01409-w [doi]
AB  - Hypodiploidy with < 40 chromosomes is associated with poor prognosis in B cell 
      precursor acute lymphoblastic leukemia. In some patients, the hypodiploid clone 
      undergoes endoreduplication, resulting in doubling of the number of chromosomes 
      and masquerades as a high hyperdiploid BCP-ALL. Karyotyping reveals metaphases 
      with 50-79 chromosomes masking the hypodiploid clone. Identifying hypodiploidy in 
      such cases requires awareness of non random alterations of chromosomal copy 
      numbers found in hypodiploid BCP-ALL. We used a systematic strategy to identify 
      masked hypodiploidy integrating targeted fluorescence in situ hybridization 
      (FISH) analysis directed towards identifying monosomies of chromosomes 7, 15 and 
      17 and flow cytometry-based ploidy analysis (FCPA). Of 445 patients diagnosed as 
      BCP ALL, 2.9% (13/445) were classified as hypodiploid including patients with 
      masked hypodiploidy. Karyotype analysis showed hypodiploidy in 3 patients, near 
      triploidy in 4 patients and normal karyotype in 6 patients. Four patients with 
      near triploid clone on karyotype showed either bimodal peak (2 patients) or 
      single low hypodiploid peak (1 patient) or only near triploid peak (1 patient) on 
      FCPA. All 6 patients with normal karyotype revealed either bimodal peak (4 
      patients) or hypodiploid peak (2 patients) on FCPA. Targeted FISH analysis 
      unmasked hypodiploid clone showing monosomies of chromosomes 7, 15 and 17 in all 
      ten patients. Our algorithm successfully identified masked hypodiploidy in 
      patients, including those with endoreduplication (4 patients) and normal 
      karyotype (6 patients). Integrating FCPA with targeted FISH analysis provides a 
      practical, sensitive and specific approach to identify masked hypodiploidy in low 
      resource settings.
CI  - (c) Indian Society of Hematology and Blood Transfusion 2021.
FAU - Gupta, Tanvi
AU  - Gupta T
AD  - Department of Cytogenetics and Lab Hematology, Tata Medical Center, Kolkata, 
      India. GRID: grid.430884.3. ISNI: 0000 0004 1770 8996
FAU - Arun, S R
AU  - Arun SR
AD  - Department of Cytogenetics and Lab Hematology, Tata Medical Center, Kolkata, 
      India. GRID: grid.430884.3. ISNI: 0000 0004 1770 8996
FAU - Babu, G Ashish
AU  - Babu GA
AD  - Department of Cytogenetics and Lab Hematology, Tata Medical Center, Kolkata, 
      India. GRID: grid.430884.3. ISNI: 0000 0004 1770 8996
FAU - Chakrabarty, Barun Kumar
AU  - Chakrabarty BK
AD  - Department of Cytogenetics and Lab Hematology, Tata Medical Center, Kolkata, 
      India. GRID: grid.430884.3. ISNI: 0000 0004 1770 8996
FAU - Bhave, Saurabh Jayant
AU  - Bhave SJ
AD  - Department of Clinical Hematology, Tata Medical Center, Kolkata, India. GRID: 
      grid.430884.3. ISNI: 0000 0004 1770 8996
FAU - Kumar, Jeevan
AU  - Kumar J
AD  - Department of Clinical Hematology, Tata Medical Center, Kolkata, India. GRID: 
      grid.430884.3. ISNI: 0000 0004 1770 8996
FAU - Radhakrishnan, Vivek
AU  - Radhakrishnan V
AD  - Department of Clinical Hematology, Tata Medical Center, Kolkata, India. GRID: 
      grid.430884.3. ISNI: 0000 0004 1770 8996
FAU - Krishnan, Shekhar
AU  - Krishnan S
AD  - Department of Pediatric Oncology, Tata Medical Center, Kolkata, India. GRID: 
      grid.430884.3. ISNI: 0000 0004 1770 8996
FAU - Ghara, Niharendu
AU  - Ghara N
AD  - Department of Pediatric Oncology, Tata Medical Center, Kolkata, India. GRID: 
      grid.430884.3. ISNI: 0000 0004 1770 8996
FAU - Arora, Neeraj
AU  - Arora N
AD  - Department of Lab Hematology and Molecular Pathology, Tata Medical Center, 
      Kolkata, India. GRID: grid.430884.3. ISNI: 0000 0004 1770 8996
FAU - Mishra, Deepak Kumar
AU  - Mishra DK
AD  - Department of Lab Hematology and Molecular Pathology, Tata Medical Center, 
      Kolkata, India. GRID: grid.430884.3. ISNI: 0000 0004 1770 8996
FAU - Parihar, Mayur
AU  - Parihar M
AUID- ORCID: 0000-0002-8059-0914
AD  - Department of Cytogenetics and Lab Hematology, Tata Medical Center, Kolkata, 
      India. GRID: grid.430884.3. ISNI: 0000 0004 1770 8996
AD  - Department of Lab Hematology and Molecular Pathology, Tata Medical Center, 
      Kolkata, India. GRID: grid.430884.3. ISNI: 0000 0004 1770 8996
LA  - eng
PT  - Journal Article
DEP - 20210226
PL  - India
TA  - Indian J Hematol Blood Transfus
JT  - Indian journal of hematology & blood transfusion : an official journal of Indian 
      Society of Hematology and Blood Transfusion
JID - 9425818
PMC - PMC8523604
OTO - NOTNLM
OT  - BCP-ALL
OT  - Endoreduplication
OT  - FISH
OT  - Flow cytometry based ploidy analysis
OT  - Hypodiploidy
OT  - Near haploidy
EDAT- 2021/10/26 06:00
MHDA- 2021/10/26 06:01
PMCR- 2022/10/01
CRDT- 2021/10/25 06:19
PHST- 2020/11/27 00:00 [received]
PHST- 2021/02/09 00:00 [accepted]
PHST- 2021/10/25 06:19 [entrez]
PHST- 2021/10/26 06:00 [pubmed]
PHST- 2021/10/26 06:01 [medline]
PHST- 2022/10/01 00:00 [pmc-release]
AID - 1409 [pii]
AID - 10.1007/s12288-021-01409-w [doi]
PST - ppublish
SO  - Indian J Hematol Blood Transfus. 2021 Oct;37(4):576-585. doi: 
      10.1007/s12288-021-01409-w. Epub 2021 Feb 26.