PMID- 34690929
OWN - NLM
STAT- MEDLINE
DCOM- 20220214
LR  - 20220214
IS  - 1664-2392 (Print)
IS  - 1664-2392 (Electronic)
IS  - 1664-2392 (Linking)
VI  - 12
DP  - 2021
TI  - Type 2 Diabetes Modifies Skeletal Muscle Gene Expression Response to Gastric 
      Bypass Surgery.
PG  - 728593
LID - 10.3389/fendo.2021.728593 [doi]
LID - 728593
AB  - INTRODUCTION: Roux-en-Y gastric bypass (RYGB) is an effective treatment for type 
      2 diabetes mellitus (T2DM) that can result in remission of clinical symptoms, yet 
      mechanisms for improved skeletal muscle health are poorly understood. We sought 
      to define the impact of existing T2DM on RYGB-induced muscle transcriptome 
      changes. METHODS: Vastus lateralis biopsy transcriptomes were generated pre- and 
      1-year post-RYGB in black adult females with (T2D; n = 5, age = 51 +/- 6 years, BMI 
      = 53.0 +/- 5.8 kg/m(2)) and without (CON; n = 7, 43 +/- 6 years, 51.0 +/- 9.2 kg/m(2)) 
      T2DM. Insulin, glucose, and HOMA-IR were measured in blood at the same time 
      points. ANCOVA detected differentially expressed genes (p < 0.01, fold change < 
      |1.2|), which were used to identify enriched biological pathways. RESULTS: 
      Pre-RYGB, 95 probes were downregulated with T2D including subunits of 
      mitochondrial complex I. Post-RYGB, the T2D group had normalized gene expression 
      when compared to their non-diabetic counterparts with only three probes remaining 
      significantly different. In the T2D, we identified 52 probes upregulated from 
      pre- to post-RYGB, including NDFUB7 and NDFUA1. CONCLUSION: Black females with 
      T2DM show extensive downregulation of genes across aerobic metabolism pathways 
      prior to RYGB, which resolves 1 year post-RYGB and is related to improvements in 
      clinical markers. These data support efficacy of RYGB for improving skeletal 
      muscle health, especially in patients with T2DM.
CI  - Copyright (c) 2021 Barberio, Dohm, Pories, Gadaleta, Houmard, Nadler and Hubal.
FAU - Barberio, Matthew D
AU  - Barberio MD
AD  - Center for Genetic Medicine Research, Children's National Research Institute, 
      Washington, DC, United States.
AD  - Department of Exercise and Nutrition Sciences, Milken Institute School of Public 
      Health, George Washington University, Washington, DC, United States.
FAU - Dohm, G Lynis
AU  - Dohm GL
AD  - Department of Physiology, Brody School of Medicine, East Carolina University, 
      Greenville, NC, United States.
FAU - Pories, Walter J
AU  - Pories WJ
AD  - Department of Surgery, Brody School of Medicine, East Carolina University, 
      Greenville, NC, United States.
FAU - Gadaleta, Natalie A
AU  - Gadaleta NA
AD  - Department of Exercise and Nutrition Sciences, Milken Institute School of Public 
      Health, George Washington University, Washington, DC, United States.
FAU - Houmard, Joseph A
AU  - Houmard JA
AD  - Human Performance Laboratory, Department of Kinesiology, College of Health and 
      Human Performance, East Carolina University, Greenville, NC, United States.
FAU - Nadler, Evan P
AU  - Nadler EP
AD  - Division of Pediatric Surgery, Children's National Hospital, Washington, DC, 
      United States.
FAU - Hubal, Monica J
AU  - Hubal MJ
AD  - Center for Genetic Medicine Research, Children's National Research Institute, 
      Washington, DC, United States.
AD  - Department of Kinesiology, Indiana University Purdue University Indianapolis, 
      Indianapolis, IN, United States.
LA  - eng
GR  - UL1 TR000075/TR/NCATS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20211006
PL  - Switzerland
TA  - Front Endocrinol (Lausanne)
JT  - Frontiers in endocrinology
JID - 101555782
SB  - IM
MH  - Adult
MH  - Biopsy
MH  - Case-Control Studies
MH  - Diabetes Mellitus, Type 2/complications/genetics/pathology/*surgery
MH  - Female
MH  - *Gastric Bypass
MH  - Gene Expression
MH  - Gene Expression Profiling
MH  - Humans
MH  - Middle Aged
MH  - Muscle, Skeletal/*metabolism/pathology
MH  - Obesity/complications/genetics/pathology/surgery
MH  - *Transcriptome
MH  - Treatment Outcome
MH  - United States
PMC - PMC8526857
OTO - NOTNLM
OT  - bariatric (weight-loss) surgery
OT  - gene expression
OT  - metabolism
OT  - skeletal muscle
OT  - type 2 diabetes (T2D)
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/10/26 06:00
MHDA- 2022/02/15 06:00
PMCR- 2021/01/01
CRDT- 2021/10/25 06:24
PHST- 2021/06/21 00:00 [received]
PHST- 2021/09/13 00:00 [accepted]
PHST- 2021/10/25 06:24 [entrez]
PHST- 2021/10/26 06:00 [pubmed]
PHST- 2022/02/15 06:00 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fendo.2021.728593 [doi]
PST - epublish
SO  - Front Endocrinol (Lausanne). 2021 Oct 6;12:728593. doi: 
      10.3389/fendo.2021.728593. eCollection 2021.