PMID- 34692369
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221102
IS  - 2190-572X (Print)
IS  - 2190-5738 (Electronic)
IS  - 2190-5738 (Linking)
VI  - 11
IP  - 11
DP  - 2021 Nov
TI  - Biosynthesis of polyunsaturated fatty acids by two newly cold-adapted Egyptian 
      marine yeast.
PG  - 461
LID - 10.1007/s13205-021-03010-4 [doi]
LID - 461
AB  - The widespread awareness of polyunsaturated fatty acids (PUFAs) benefits for 
      human health has increased the need for their commercial production. Two 
      oleaginous yeast were isolated from the Mediterranean Sea fish and Red Sea fish 
      Epinephelus aeneus and E. areolatus, respectively. These marine candidates were 
      identified by MALDI-TOF/MS biotyper((R)) as Lodderomyces elongisporus and 
      Rhodotorula mucilaginosa. The effect of incubation temperature (7, 15, and 26  degrees C) 
      and glucose concentration (3% and 8%) on their lipids content were investigated 
      using Sulfo-Phospho-Vanillin (SPV) assay. Their intercellular lipids were 
      visualized by fluorescence microscope using Nile-Red dye. L. elongisporus and R. 
      mucilaginosa produced 20.04% and 26.79% of Linoleic acid, respectively, on normal 
      Basal-Defatted Medium (BDM). Linoleic acid (21.4-22.7%) and alpha-Linolenic acid 
      (7.5-10.8%) were produced by R. mucilaginosa and L. elongisporus, on normal BDM 
      at 15  degrees C. High-Glucose BDM induced a positive effect on the total lipids 
      production that reached its maximum of 48% and 54% by R. mucilaginosa and L. 
      elongisporus, respectively, grown at 15  degrees C. Remarkably, 12.12% of long-chain 
      15-Docosenoic acid (C(22:1)) and 21.49% of Tricosanoic acid (C(23:0)) were 
      detected in the FAs profile of L. elongisporus, when grown on normal BDM at 
      26  degrees C. The present study is the first one reporting the FAs profile of the 
      Egyptian Marine L. elongisporus, and its capability to accumulate high amounts of 
      lipids under appropriate fermentation conditions; thus, it could be considered 
      for scaling up production. SUPPLEMENTARY INFORMATION: The online version contains 
      supplementary material available at 10.1007/s13205-021-03010-4.
CI  - (c) King Abdulaziz City for Science and Technology 2021.
FAU - Adel, Amera
AU  - Adel A
AD  - Food Safety Organization, Cairo, Egypt.
FAU - El-Baz, Ashraf
AU  - El-Baz A
AD  - Departement of Industrial Biotechnology, Genetic Engineering and Biotechnology 
      Research Institute, University of Sadat City, Sadat City, 22857/79 Egypt. GRID: 
      grid.449877.1. ISNI: 0000 0004 4652 351X
FAU - Shetaia, Yousseria
AU  - Shetaia Y
AD  - Department of Microbiology, Faculty of Science, Ain Shams University, Cairo, 
      Egypt. GRID: grid.7269.a. ISNI: 0000 0004 0621 1570
FAU - Sorour, Noha Mohamed
AU  - Sorour NM
AUID- ORCID: 0000-0002-6117-7980
AD  - Departement of Industrial Biotechnology, Genetic Engineering and Biotechnology 
      Research Institute, University of Sadat City, Sadat City, 22857/79 Egypt. GRID: 
      grid.449877.1. ISNI: 0000 0004 4652 351X
LA  - eng
PT  - Journal Article
DEP - 20211016
PL  - Germany
TA  - 3 Biotech
JT  - 3 Biotech
JID - 101565857
PMC - PMC8520562
OTO - NOTNLM
OT  - Lodderomyces elongisporus
OT  - Marine
OT  - Optimization
OT  - PUFAs
OT  - Rhodotorula mucilaginosa
OT  - Yeast
COIS- Conflict of interestAll the authors declare that there are no 
      financial/commercial conflicts of interest.
EDAT- 2021/10/26 06:00
MHDA- 2021/10/26 06:01
PMCR- 2022/11/01
CRDT- 2021/10/25 06:36
PHST- 2021/07/30 00:00 [received]
PHST- 2021/09/28 00:00 [accepted]
PHST- 2021/10/25 06:36 [entrez]
PHST- 2021/10/26 06:00 [pubmed]
PHST- 2021/10/26 06:01 [medline]
PHST- 2022/11/01 00:00 [pmc-release]
AID - 3010 [pii]
AID - 10.1007/s13205-021-03010-4 [doi]
PST - ppublish
SO  - 3 Biotech. 2021 Nov;11(11):461. doi: 10.1007/s13205-021-03010-4. Epub 2021 Oct 
      16.