PMID- 34694985
OWN - NLM
STAT- MEDLINE
DCOM- 20211027
LR  - 20220531
IS  - 1466-5034 (Electronic)
IS  - 1466-5026 (Linking)
VI  - 71
IP  - 10
DP  - 2021 Oct
TI  - Trichococcus shcherbakoviae subsp. psychrophilus subsp. nov., a psychrotolerant 
      facultative anaerobe isolated from a cold spring.
LID - 10.1099/ijsem.0.005068 [doi]
AB  - A psychrotolerant facultative anaerobe, strain SKBG(T), was isolated from the 
      bottom sediments of the cold mineral spring Buxichen (Buryatia, Russia). 
      Gram-positive non-motile cocci with a diameter of 1.75-2.5 microm were observed 
      singly or in long chains. Cells grew in the temperature range from ̶ 5-35  degrees C. 
      Growth was observed within the pH range of 7.0-9.5, with the optimum growth at pH 
      7.6 and at a NaCl concentration from 0-1.0 % (optimum 0.1 % (w/v)). Strain 
      SKBG(T) was a chemoorganoheterotroph that used sugars and some organic acids as 
      substrates. The predominant fatty acids in cell walls were capital ES, Cyrillic(16:1)omega9, capital ES, Cyrillic(18:1)omega9, 
      and capital ES, Cyrillic(16 : 0). The 16S rRNA gene sequence of strain SKBG(T) shared high 
      similarity (>99 %) with those of the type strains of the genus Trichococcus. 
      Digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values 
      between strain SKBG(T) and Trichococcus shcherbakoviae Art(T) (=DSM 107162(T)=VKM 
      B-3260(T)) were 70.1 and 95.4 %, respectively. The genomic DNA G+C content of 
      strain SKBG(T) was 47.1 mol%. Compared with the type strain of T. shcherbakoviae, 
      the new strain was characterized by a temperature optimum for growth (10  degrees C) 
      significantly lower than that of T. shcherbakoviae DSM 107162(T) (20-30  degrees C). 
      Based on phenotypic and genomic characteristics, the isolate SKBG(T) was 
      classified as T. shcherbakoviae subsp. psychrophilus subsp. nov. The type strain 
      is SKBG(T) (=VKM B-3241(capital TE, Cyrillic)=JCM 33326(T)).
FAU - Zakharyuk, Anastasiya
AU  - Zakharyuk A
AD  - Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal 
      Research Center "Pushchino Scientific Center for Biological Research of the 
      Russian Academy of Sciences", Prospect Nauki 5, 142290, Pushchino, Moscow Region, 
      Russia.
FAU - Valyshev, Alexander
AU  - Valyshev A
AD  - Institute of Cellular and Intracellular Symbiosis of the Ural Branch of the 
      Russian Academy of Sciences, Pionerskaya street, 11, 460000, Orenburg, Russia.
FAU - Plotnikov, Andrey
AU  - Plotnikov A
AD  - Institute of Cellular and Intracellular Symbiosis of the Ural Branch of the 
      Russian Academy of Sciences, Pionerskaya street, 11, 460000, Orenburg, Russia.
FAU - Kopitsyn, Dmitry
AU  - Kopitsyn D
AD  - Gubkin University, Leninsky Prospect 65-1, 119991, Moscow, Russia.
FAU - Suzina, Nataliya
AU  - Suzina N
AD  - Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal 
      Research Center "Pushchino Scientific Center for Biological Research of the 
      Russian Academy of Sciences", Prospect Nauki 5, 142290, Pushchino, Moscow Region, 
      Russia.
FAU - Shcherbakova, Viktoria
AU  - Shcherbakova V
AD  - Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal 
      Research Center "Pushchino Scientific Center for Biological Research of the 
      Russian Academy of Sciences", Prospect Nauki 5, 142290, Pushchino, Moscow Region, 
      Russia.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Syst Evol Microbiol
JT  - International journal of systematic and evolutionary microbiology
JID - 100899600
RN  - 0 (DNA, Bacterial)
RN  - 0 (Fatty Acids)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Bacterial Typing Techniques
MH  - Base Composition
MH  - Carnobacteriaceae/*classification/isolation & purification
MH  - DNA, Bacterial/genetics
MH  - Fatty Acids/chemistry
MH  - Natural Springs/*microbiology
MH  - Nucleic Acid Hybridization
MH  - *Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Russia
MH  - Sequence Analysis, DNA
OTO - NOTNLM
OT  - Trichococcus sp
OT  - cold spring
OT  - genomic analysis
OT  - psychrotolerant
OT  - taxonomy
EDAT- 2021/10/26 06:00
MHDA- 2021/10/28 06:00
CRDT- 2021/10/25 17:17
PHST- 2021/10/25 17:17 [entrez]
PHST- 2021/10/26 06:00 [pubmed]
PHST- 2021/10/28 06:00 [medline]
AID - 10.1099/ijsem.0.005068 [doi]
PST - ppublish
SO  - Int J Syst Evol Microbiol. 2021 Oct;71(10). doi: 10.1099/ijsem.0.005068.