PMID- 34695652
OWN - NLM
STAT- MEDLINE
DCOM- 20220301
LR  - 20240226
IS  - 1532-3072 (Electronic)
IS  - 0040-8166 (Linking)
VI  - 73
DP  - 2021 Dec
TI  - Cardiomyocyte-like cell differentiation by FGF-2 transfection and induction of 
      rat bone marrow mesenchymal stem cells.
PG  - 101665
LID - S0040-8166(21)00181-6 [pii]
LID - 10.1016/j.tice.2021.101665 [doi]
AB  - OBJECTIVE(S): To investigate and test the hypotheses that FGF-2 enhanced 
      myocardial differentiation with rat bone marrow mesenchymal stem cells (BMSCs). 
      MATERIALS AND METHODS: Lentiviral vectors carrying the FGF-2 gene were 
      transfected into rat BMSCs firstly. According to the different inducing agents, 
      they were divided into the following four groups: group A (BMSCs blank control 
      group), group B (FGF-2 induction group), group C (Lenti-FGF-2-GFP lentivirus 
      transfection group), and the group D (Lenti-control-GFP lentiviral transfer). 
      Then several kinds of experimental methods such as real-time PCR, 
      immunocytochemical staining, immunofluorescence staining, Western blot, and 
      transmission electron microscopy were used to elucidate the effects by which 
      FGF-2 adjusts myocardial differentiation in rat BMSCs. RESULTS: The results of 
      real-time PCR showed that GATA-4 and Nkx2.5 were expressed in all groups of 
      cells. Compared with the experimental control group, the expression of GATA-4 and 
      Nkx2.5 genes was the strongest after induction of 2 weeks in each induction 
      group, and gradually decreased after induction of 4 weeks. Among them, the 
      relative expression levels of GATA-4 and Nkx2.5 genes in Lenti-FGF-2-GFP were 
      highest at all time points. The expressions of cTnI, cTnT, Cx43, and Desmin were 
      detected by immunocytochemical staining and immunofluorescence staining. After 4 
      weeks of induction, cTnI, cTnT, Cx43, and Desmin were positively expressed in the 
      cytoplasm of cells. Statistical analysis showed that the integrated optical 
      density (IOD) values of the markers in the Lenti-FGF-2-GFP were the strongest. 
      Cx43 and cTnI were weakly positive or negative in the experimental control group. 
      There was a significant difference in the positive expression of each marker in 
      each induction group and the experimental control group. Western blot analysis 
      showed that Tromyosin (Tm) and Desmin were expressed in the blank group, FGF-2 
      drug-induced group, Lenti-FGF-2-GFP, and empty virus control transfection group 
      after 4 weeks of induction, among which FGF-2 lentivirus transfected. The 
      expression levels of Tm and Desmin were the highest in the staining induction 
      group. Statistical analysis showed that the positive expressions of Tm and Desmin 
      in each experimental group were statistically significant. Transmission electron 
      microscopy showed that the nucleus of the cells transfected and induced by FGF-2 
      was located at the center of the cells. Myofilaments, rough endoplasmic 
      reticulum, and mitochondria, and ribosomes were seen in the cytoplasm. 
      CONCLUSION: These results indicate that FGF-2 can transfect and induce 
      differentiation of BMSCs into cardiomyocyte-like cells. Lentivirus-mediated FGF-2 
      transfection induces the differentiation of bone marrow mesenchymal stem cells 
      into cardiomyocyte-like cells better than FGF-2 direct induction.
CI  - Copyright (c) 2021. Published by Elsevier Ltd.
FAU - Li, Jiao
AU  - Li J
AD  - Department of Histology and Embryology, Hebei North University, Zhangjiakou city, 
      China.
FAU - Lv, Yang
AU  - Lv Y
AD  - Department of Histology and Embryology, Hebei North University, Zhangjiakou city, 
      China.
FAU - Wang, Haoyu
AU  - Wang H
AD  - Department of Histology and Embryology, Hebei North University, Zhangjiakou city, 
      China.
FAU - Liu, Yang
AU  - Liu Y
AD  - Department of Histology and Embryology, Hebei North University, Zhangjiakou city, 
      China.
FAU - Ren, Junxu
AU  - Ren J
AD  - Department of Histology and Embryology, Hebei North University, Zhangjiakou city, 
      China.
FAU - Wang, Haiping
AU  - Wang H
AD  - Department of Histology and Embryology, Hebei North University, Zhangjiakou city, 
      China. Electronic address: hbbfxy_whp@163.com.
LA  - eng
PT  - Journal Article
DEP - 20211014
PL  - Scotland
TA  - Tissue Cell
JT  - Tissue & cell
JID - 0214745
RN  - 0 (Desmin)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Animals
MH  - *Cell Differentiation
MH  - Cell Shape
MH  - Desmin/metabolism
MH  - Fibroblast Growth Factor 2/*metabolism
MH  - Gene Expression Regulation
MH  - Mesenchymal Stem Cells/*metabolism/ultrastructure
MH  - Myocytes, Cardiac/*cytology/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats, Sprague-Dawley
MH  - Transcription Factors/genetics/metabolism
MH  - Transfection
MH  - Rats
OTO - NOTNLM
OT  - Bone marrow mesenchymal stem cell
OT  - Cardiomyocytes
OT  - Fibroblast growth factor 2
OT  - Lentivirus transfection
EDAT- 2021/10/26 06:00
MHDA- 2022/03/03 06:00
CRDT- 2021/10/25 20:17
PHST- 2021/04/04 00:00 [received]
PHST- 2021/10/04 00:00 [revised]
PHST- 2021/10/05 00:00 [accepted]
PHST- 2021/10/26 06:00 [pubmed]
PHST- 2022/03/03 06:00 [medline]
PHST- 2021/10/25 20:17 [entrez]
AID - S0040-8166(21)00181-6 [pii]
AID - 10.1016/j.tice.2021.101665 [doi]
PST - ppublish
SO  - Tissue Cell. 2021 Dec;73:101665. doi: 10.1016/j.tice.2021.101665. Epub 2021 Oct 
      14.