PMID- 34698457
OWN - NLM
STAT- MEDLINE
DCOM- 20211027
LR  - 20211027
IS  - 1000-0607 (Print)
IS  - 1000-0607 (Linking)
VI  - 46
IP  - 10
DP  - 2021 Oct 25
TI  - [Effect of electroacupuncture combined with Zhuang-medicine-thread moxibustion on 
      silent information regulator-1/nuclear factor kappaB signaling pathway in gastric 
      antrum of diabetic gastroparesis rats].
PG  - 837-44
LID - 10.13702/j.1000-0607.200942 [doi]
AB  - OBJECTIVE: To observe the effect of electroacupuncture (EA) combined with 
      Zhuang-medicine-thread moxibustion on silent information regulator-1 
      (SIRT1)/nuclear factor kappa B (NF-kappaB) signal pathway and inflammatory factor 
      expression in gastric antrum tissue of diabetic gastroparesis (DGP) rats, so as 
      to explore its mechanism underlying improvement of DGP. METHODS: Male SD rats 
      were randomly divided into normal, model, medication, EA, Zhuang-medicine-thread 
      moxibustion (moxibustion) and EA+moxibustion groups (n=12 per group). The DGP 
      model was established by intraperitoneal injection of streptozotocin (STZ). Rats 
      of the medication group were treated by gavage of 0.15 mg/mL mosapride citrate 
      suspension. EA (10 Hz /50 Hz, 2 mA) or moxibustion (3 cones) or EA+moxibustion 
      was applied to "Zhongwan"(CV12), bilateral "Neiguan"(PC6) and bilateral 
      "Sanyinjiao"(SP6) of the related group for 20 min, once a day for 3 weeks. Blood 
      glucose, gastric emptying rate and intestinal propulsion rate were measured. The 
      levels of serum interleukin (IL)-6, IL-8, IL-10 and tumor necrosis 
      factor-alpha(TNF-alpha) were detected by ELISA; the phosphorylation level of the 
      phosphorylated inhibitor of nuclear factor kappaBalpha inhibitor (pIkappa-Balpha), the protein 
      and mRNA expression of NF-kappaB p65 and SIRT1 in the gastric antrum tissue were 
      detected by Western blot and real-time quantifitative PCR, respectively. RESULTS: 
      (1) Compared with the normal group, the levels of blood glucose, serum IL-6, 
      IL-8, TNF-alpha, and gastric pIkappa-Balpha and NF-kappaB p65 protein and mRNA expressions were 
      significantly increased (P<0.01), and the gastric emptying rate, intestinal 
      propulsion rate, serum IL-10 level, and SIRT1 protein and mRNA expressions were 
      considerably decreased in the model group (P<0.01). (2) In contrast to the model 
      group, the blood glucose in the EA, moxibustion and EA+moxibustion groups, serum 
      IL-6, IL-8 and TNF-alpha levels in the 4 treatment groups, as well as NF-kappaB p65 
      protein expression in the medication and EA+moxibustion groups, and NF-kappaB p65 
      mRNA expression and pIkappa-Balpha protein and mRNA expression in the 4 treatment groups 
      were significantly decreased (P<0.01, P<0.05); while the gastric emptying rate 
      and intestinal propulsive rate and IL-10 content in the 4 treatment groups, and 
      SIRT1 protein and mRNA expression in the medication and EA+moxibustion groups 
      were obviously increased (P<0.05, P<0.01). (3) The effects of EA+moxibustion were 
      significantly superior to those of simple EA and moxibustion in increasing 
      gastric emptying rate, IL-10, SIRT1 protein expression (P<0.05, P<0.01), and in 
      lowering IL-8 and TNF-alpha contents, pIkappa-Balpha protein and mRNA expression and NF-kappaB 
      p65 mRNA expression (P<0.05, P<0.01). No significant differences were found among 
      the 4 intervention groups in promoting the intestinal propulsive rate and among 
      the EA, moxibustion and EA+moxibustion groups in lowering blood glucose (P>0.05). 
      CONCLUSION: EA combined with Zhuang-medicine-thread moxibustion can effectively 
      reduce the level of serum inflammatory factors and regulate SIRT1/NF-kappaB signal 
      pathway in DGP rats, which may contribute to its function in improving 
      gastrointestinal movement.
FAU - Mai, Wei
AU  - Mai W
AD  - Graduate School, Guangxi University of Chinese Medicine, Nanning 530001, China.
FAU - Fan, Yu-Shan
AU  - Fan YS
AD  - College of Acupuncture-moxibustion and Tuina, Guangxi University of Chinese 
      Medicine, Nanning 530001, China.
FAU - Miao, Fu-Rui
AU  - Miao FR
AD  - Graduate School, Guangxi University of Chinese Medicine, Nanning 530001, China.
FAU - He, Cai
AU  - He C
AD  - Graduate School, Guangxi University of Chinese Medicine, Nanning 530001, China.
FAU - Huang, Li-Lin
AU  - Huang LL
AD  - Graduate School, Guangxi University of Chinese Medicine, Nanning 530001, China.
FAU - Zhao, Xiao-Jun
AU  - Zhao XJ
AD  - Graduate School, Guangxi University of Chinese Medicine, Nanning 530001, China.
FAU - Nie, Ying-Jie
AU  - Nie YJ
AD  - Graduate School, Guangxi University of Chinese Medicine, Nanning 530001, China.
FAU - Pang, Rui-Kang
AU  - Pang RK
AD  - Graduate School, Guangxi University of Chinese Medicine, Nanning 530001, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhen Ci Yan Jiu
JT  - Zhen ci yan jiu = Acupuncture research
JID - 8507710
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Acupuncture Points
MH  - Animals
MH  - *Diabetes Mellitus
MH  - *Electroacupuncture
MH  - *Gastroparesis/genetics/therapy
MH  - Male
MH  - *Moxibustion
MH  - NF-kappa B/genetics/metabolism
MH  - Pyloric Antrum/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
OTO - NOTNLM
OT  - Diabetic gastroparesis
OT  - Electroacupuncture
OT  - Gastric SIRT1/NF-kappaB signaling
OT  - Gastrointestinal movement
OT  - Inflammatory factors
OT  - Zhuang-medicine-thread moxibustion
EDAT- 2021/10/27 06:00
MHDA- 2021/10/28 06:00
CRDT- 2021/10/26 09:43
PHST- 2021/10/26 09:43 [entrez]
PHST- 2021/10/27 06:00 [pubmed]
PHST- 2021/10/28 06:00 [medline]
AID - 10.13702/j.1000-0607.200942 [doi]
PST - ppublish
SO  - Zhen Ci Yan Jiu. 2021 Oct 25;46(10):837-44. doi: 10.13702/j.1000-0607.200942.