PMID- 34698625
OWN - NLM
STAT- MEDLINE
DCOM- 20211028
LR  - 20220531
IS  - 1466-5034 (Electronic)
IS  - 1466-5026 (Linking)
VI  - 71
IP  - 10
DP  - 2021 Oct
TI  - Staphylococcus singaporensis sp. nov., a new member of the Staphylococcus aureus 
      complex, isolated from human clinical specimens.
LID - 10.1099/ijsem.0.005067 [doi]
AB  - Staphylococcus argenteus and Staphylococcus schweitzeri are the newest members of 
      the Staphylococcus aureus complex. The number of clinical reports attributed to 
      these new S. aureus complex members is limited. In a retrospective clinical 
      laboratory study conducted over a 4-month period investigating the prevalence of 
      S. argenteus and S. schweitzeri, a total of 43 isolates were selected. Phylogeny 
      based on core-gene multilocus sequence typing (MLST) analysis confirmed that 37 
      were S. argenteus but a genetically distinct clade of six isolates was 
      identified. Digital DNA-DNA hybridization (dDDH) and average nucleotide identity 
      (ANI) analyses further supported the classification of these six isolates as a 
      separate species. When compared to S. aureus complex reference genomes, the ANI 
      values were </=94 % and the dDDH values were <53 %. Based on the seven-gene S. 
      aureus MLST scheme, the six isolates belong to five novel allelic profiles 
      (ST6105, ST6106, ST6107, ST6108 and ST109). Their clinical infection features 
      were similar to S. aureus. Skin and soft tissue infections presented in four out 
      of the six cases. Routine clinical diagnostic identification using 
      matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 
      biochemical profiling does not differentiate these new members from the rest of 
      the complex. Genotypic analysis suggests that the six isolates belong to a novel 
      species, Staphylococcus singaporensis sp. nov. with isolate SS21(T) (=DSM 
      111408(T)=NCTC14419(T)) designated as the type strain.
FAU - Chew, Ka Lip
AU  - Chew KL
AD  - Department of Laboratory Medicine, National University Hospital, Singapore, 
      Singapore.
FAU - Octavia, Sophie
AU  - Octavia S
AD  - National Public Health Laboratory, National Centre for Infectious Diseases, 
      Singapore, Singapore.
AD  - Present address: Environmental Health Institute, National Environment Agency, 
      Singapore, Singapore.
FAU - Lai, Deborah
AU  - Lai D
AD  - Department of Pathology, Singapore General Hospital, Singapore, Singapore.
FAU - Lin, Raymond T P
AU  - Lin RTP
AD  - Department of Laboratory Medicine, National University Hospital, Singapore, 
      Singapore.
AD  - National Public Health Laboratory, National Centre for Infectious Diseases, 
      Singapore, Singapore.
FAU - Teo, Jeanette W P
AU  - Teo JWP
AD  - Department of Laboratory Medicine, National University Hospital, Singapore, 
      Singapore.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Syst Evol Microbiol
JT  - International journal of systematic and evolutionary microbiology
JID - 100899600
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - Staphylococcus argenteus
RN  - Staphylococcus schweitzeri
SB  - IM
MH  - Bacterial Typing Techniques
MH  - Base Composition
MH  - DNA, Bacterial/genetics
MH  - Humans
MH  - Multilocus Sequence Typing
MH  - Nucleic Acid Hybridization
MH  - *Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Retrospective Studies
MH  - Sequence Analysis, DNA
MH  - Staphylococcus/*classification/isolation & purification
MH  - Staphylococcus aureus/genetics
OTO - NOTNLM
OT  - Staphylococcus argenteus
OT  - Staphylococcus aureus complex
OT  - clinical features
OT  - phenotypic typing
OT  - whole-genome-sequencing
EDAT- 2021/10/27 06:00
MHDA- 2021/10/29 06:00
CRDT- 2021/10/26 12:20
PHST- 2021/10/26 12:20 [entrez]
PHST- 2021/10/27 06:00 [pubmed]
PHST- 2021/10/29 06:00 [medline]
AID - 10.1099/ijsem.0.005067 [doi]
PST - ppublish
SO  - Int J Syst Evol Microbiol. 2021 Oct;71(10). doi: 10.1099/ijsem.0.005067.