PMID- 34704665
OWN - NLM
STAT- MEDLINE
DCOM- 20220505
LR  - 20220505
IS  - 1862-8354 (Electronic)
IS  - 1862-8346 (Linking)
VI  - 16
IP  - 2
DP  - 2022 Mar
TI  - Screening of differentially expressed proteins in placentas from patients with 
      late-onset preeclampsia.
PG  - e2100053
LID - 10.1002/prca.202100053 [doi]
AB  - PURPOSE: Preeclampsia (PE) is a severe disease that endangers the safety of 
      mothers and fetuses worldwide. In the absence of specific treatments, more 
      studies on novel predictive and diagnostic biomarkers for PE are required. 
      EXPERIMENTAL DESIGN: Data-independent acquisition proteomics, with five 
      biological replicates, was used to investigate the protein expression profiles of 
      placental tissues from patients with PE and normal pregnant women. RESULTS: In 
      total, 52 differentially expressed proteins (DEPs) were identified, 34 of them 
      were upregulated and 18 downregulated. Bioinformatics analyses revealed that PE 
      was associated with multiple GO terms and KEGG pathways. Arginase-1 (ARG1), 
      ferritin light chain (FTL), and RNA cytidine acetyltransferase (NAT10) were 
      identified as hub proteins, which were further validated in placental tissues and 
      maternal plasma by western blot and ELISA. CONCLUSIONS AND CLINICAL RELEVANCE: 
      FTL expression was significantly lower in the placental tissues and early and 
      late pregnancy plasma of patients with PE compared to that in normal pregnant 
      women. This study is the first to propose that FTL may be a potential predictive 
      and diagnostic biomarker for PE; it provides a proteomics insight for 
      understanding the pathological mechanism of this disease.
CI  - (c) 2021 Wiley-VCH GmbH.
FAU - He, Andong
AU  - He A
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan 
      University, Guangzhou, China.
AD  - International Joint Laboratory for Embryonic Development & Prenatal Medicine, 
      Division of Histology and Embryology, Medical College, Jinan University, 
      Guangzhou, China.
FAU - Wang, Jingyun
AU  - Wang J
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan 
      University, Guangzhou, China.
AD  - International Joint Laboratory for Embryonic Development & Prenatal Medicine, 
      Division of Histology and Embryology, Medical College, Jinan University, 
      Guangzhou, China.
FAU - Yang, Xiaofeng
AU  - Yang X
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan 
      University, Guangzhou, China.
AD  - International Joint Laboratory for Embryonic Development & Prenatal Medicine, 
      Division of Histology and Embryology, Medical College, Jinan University, 
      Guangzhou, China.
FAU - Liu, Jia
AU  - Liu J
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan 
      University, Guangzhou, China.
FAU - Yang, Xuesong
AU  - Yang X
AD  - International Joint Laboratory for Embryonic Development & Prenatal Medicine, 
      Division of Histology and Embryology, Medical College, Jinan University, 
      Guangzhou, China.
AD  - Key Laboratory for Regenerative Medicine of the Ministry of Education, Jinan 
      University, Guangzhou, China.
AD  - Guangdong-Hong Kong Metabolism & Reproduction Joint Laboratory, Medical College, 
      Jinan University, Guangzhou, China.
FAU - Wang, Guang
AU  - Wang G
AUID- ORCID: 0000-0001-7595-2880
AD  - International Joint Laboratory for Embryonic Development & Prenatal Medicine, 
      Division of Histology and Embryology, Medical College, Jinan University, 
      Guangzhou, China.
AD  - Key Laboratory for Regenerative Medicine of the Ministry of Education, Jinan 
      University, Guangzhou, China.
AD  - Guangdong-Hong Kong Metabolism & Reproduction Joint Laboratory, Medical College, 
      Jinan University, Guangzhou, China.
FAU - Li, Ruiman
AU  - Li R
AUID- ORCID: 0000-0003-1239-7968
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan 
      University, Guangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211110
PL  - Germany
TA  - Proteomics Clin Appl
JT  - Proteomics. Clinical applications
JID - 101298608
RN  - 0 (Proteins)
SB  - IM
MH  - Computational Biology
MH  - Female
MH  - Humans
MH  - Placenta/metabolism
MH  - *Pre-Eclampsia/diagnosis/genetics
MH  - Pregnancy
MH  - Proteins/metabolism
MH  - Proteomics
OTO - NOTNLM
OT  - bioinformatics
OT  - biomarker
OT  - ferritin light chain
OT  - preeclampsia
OT  - proteomics
EDAT- 2021/10/28 06:00
MHDA- 2022/05/06 06:00
CRDT- 2021/10/27 08:53
PHST- 2021/09/26 00:00 [revised]
PHST- 2021/07/18 00:00 [received]
PHST- 2021/10/22 00:00 [accepted]
PHST- 2021/10/28 06:00 [pubmed]
PHST- 2022/05/06 06:00 [medline]
PHST- 2021/10/27 08:53 [entrez]
AID - 10.1002/prca.202100053 [doi]
PST - ppublish
SO  - Proteomics Clin Appl. 2022 Mar;16(2):e2100053. doi: 10.1002/prca.202100053. Epub 
      2021 Nov 10.