PMID- 34706435
OWN - NLM
STAT- MEDLINE
DCOM- 20211101
LR  - 20211101
IS  - 1573-2517 (Electronic)
IS  - 0165-0327 (Linking)
VI  - 295
DP  - 2021 Dec 1
TI  - Sex differences in the transcription of monoamine transporters in major 
      depression.
PG  - 1215-1219
LID - S0165-0327(21)00929-0 [pii]
LID - 10.1016/j.jad.2021.08.124 [doi]
AB  - BACKGROUND: Accumulating evidence indicates that reduced activity within the 
      monoamine systems contributes to the pathophysiology of major depressive disorder 
      (MDD) and suicide. In this study, we have tested the hypothesis that 
      monoaminergic gene transcription is abnormally regulated in MDD and suicide. 
      METHODS: The transcription of specific monoaminergic genes was quantified by qPCR 
      in the dorsolateral prefrontal cortex (DLPFC) of postmortem MDD subjects (n = 80) 
      and non-psychiatric controls (CTRL, n = 32). We measured transcripts encoding 
      monoaminergic transporters (the serotonin transporter (SERT), norepinephrine 
      transporter (NET), dopamine transporter (DAT), plasma monoamine transporter 
      (PMAT), vesicular monoamine transporter (VMAT)) in addition to the tryptophan 
      hydroxylase (TPH) enzymes, TPH1 and TPH2. We tested for transcriptional 
      differences between diagnostic groups and tested for differences in the depressed 
      suicides. RESULTS: Multivariate analysis of monoaminergic gene transcription 
      revealed a sex by diagnosis interaction (F(8,99) = 2.87, p = 0.007). We report 
      lower VMAT1 and PMAT expression in depressed males, and conversely higher VMAT2, 
      TPH2 and NET expression in depressed females, relative to controls of the same 
      sex (p < 0.05). We did not detect differences in monoamine gene transcription 
      between the depressed suicides and depressed non-suicides. LIMITATIONS: Gene 
      expression measures were not associated with the presence of antidepressant 
      medication. Nevertheless, to minimize the impact of medication status and other 
      potential confounding variables, these were included as covariates in our 
      analyses. CONCLUSIONS: We report sex differences in the transcription of 
      monoaminergic genes in the DLPFC in MDD. Therefore abnormalities of monoaminergic 
      gene expression may contribute to altered DLPFC activity exhibited in major 
      depression.
CI  - Copyright (c) 2021. Published by Elsevier B.V.
FAU - Bristow, Greg C
AU  - Bristow GC
AD  - School of Pharmacy and Medical Sciences, University of Bradford, Bradford BD7 
      1DP, UK.
FAU - Eisenlohr-Moul, Tory
AU  - Eisenlohr-Moul T
AD  - Dept. Psychiatry, University of Illinois at Chicago, Chicago, IL 60612, USA.
FAU - Lotesto, Krista
AU  - Lotesto K
AD  - Dept. Molecular Pharmacology and Neuroscience, Stritch School of Medicine, Loyola 
      University Chicago, 2160 S 1st Ave, Maywood, IL 60153, USA.
FAU - Sodhi, Monsheel S
AU  - Sodhi MS
AD  - Dept. Molecular Pharmacology and Neuroscience, Stritch School of Medicine, Loyola 
      University Chicago, 2160 S 1st Ave, Maywood, IL 60153, USA.. Electronic address: 
      msodhi@luc.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210903
PL  - Netherlands
TA  - J Affect Disord
JT  - Journal of affective disorders
JID - 7906073
RN  - EC 1.14.16.4 (TPH2 protein, human)
RN  - EC 1.14.16.4 (Tryptophan Hydroxylase)
SB  - IM
MH  - Depression
MH  - *Depressive Disorder, Major/genetics
MH  - Female
MH  - Humans
MH  - Male
MH  - Prefrontal Cortex
MH  - Sex Characteristics
MH  - *Suicide
MH  - Tryptophan Hydroxylase/genetics
OTO - NOTNLM
OT  - Dopamine
OT  - Dorsolateral prefrontal cortex
OT  - Norepinephrine
OT  - Postmortem
OT  - Serotonin
OT  - mRNA
EDAT- 2021/10/29 06:00
MHDA- 2021/11/03 06:00
CRDT- 2021/10/28 01:00
PHST- 2020/11/09 00:00 [received]
PHST- 2021/08/03 00:00 [revised]
PHST- 2021/08/29 00:00 [accepted]
PHST- 2021/10/28 01:00 [entrez]
PHST- 2021/10/29 06:00 [pubmed]
PHST- 2021/11/03 06:00 [medline]
AID - S0165-0327(21)00929-0 [pii]
AID - 10.1016/j.jad.2021.08.124 [doi]
PST - ppublish
SO  - J Affect Disord. 2021 Dec 1;295:1215-1219. doi: 10.1016/j.jad.2021.08.124. Epub 
      2021 Sep 3.