PMID- 34709596
OWN - NLM
STAT- MEDLINE
DCOM- 20220301
LR  - 20221029
IS  - 1678-4405 (Electronic)
IS  - 1517-8382 (Print)
IS  - 1517-8382 (Linking)
VI  - 53
IP  - 1
DP  - 2022 Mar
TI  - Development of multiplex TaqMan qPCR for simultaneous detection and 
      differentiation of eight common swine viral and bacterial pathogens.
PG  - 359-368
LID - 10.1007/s42770-021-00633-w [doi]
AB  - It is laborious to diagnose the infections of classical swine fever virus (CSFV), 
      porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus 
      type 2 (PCV2), and Suid herpesvirus 1 (SuHV-1) because of the similar clinical 
      symptoms in piglets. Staphylococcus aureus (S. aureus), Streptococcus suis (S. 
      suis), Salmonella choleraesuis (S. choleraesuis, serotype: 6,7:c:1,5), and 
      Escherichia coli (E. coli) are common secondary bacterial pathogens in viral 
      infections. Furthermore, the mixed infection of these viral and bacterial 
      pathogens is more and more common in practical swine breeding. Therefore, a 
      TaqMan multiplex qPCR method for simultaneous detection and differentiation of 
      their pathogen was established in this study by designing specific primers and 
      probes for the E2 gene of CSFV, the ORF7 gene of PRRSV, the ORF1 gene of PCV2 and 
      the gE gene of SuHV-1, the nuc gene of S. aureus, the ef-tu gene of S. suis, the 
      ivnA gene of S. choleraesuis, and the 23S rRNA gene of E. coli, and its 
      specificity, sensitivity, and reproducibility were subsequently tested. The 
      results showed that TaqMan multiplex qPCR method showed a high specificity with 
      no cross reaction between different viruses, and a good repeatability with its 
      coefficient of variation lower than 5%. Besides, the sensitivity of this method 
      was also at least 10 times higher compared with conventional PCR. Overall, this 
      study provided a reliable multiplex TaqMan qPCR method for the diagnosis and 
      differentiation of the mentioned pathogens in pigs, laying a certain technical 
      basis for disease prevention and control.
CI  - (c) 2021. Sociedade Brasileira de Microbiologia.
FAU - Zhang, Qi
AU  - Zhang Q
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, 
      Shaanxi, China.
FAU - Yang, Feng
AU  - Yang F
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, 
      Shaanxi, China.
FAU - Gao, Jie
AU  - Gao J
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, 
      Shaanxi, China.
FAU - Zhang, Weimin
AU  - Zhang W
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, 
      Shaanxi, China. 313310625@qq.com.
FAU - Xu, Xingang
AU  - Xu X
AUID- ORCID: 0000-0003-0318-9104
AD  - College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, 
      Shaanxi, China. tiger2003@nwsuaf.edu.cn.
LA  - eng
GR  - 2019NY-081/the innovation project for agro-technology of Shaanxi Province/
PT  - Journal Article
DEP - 20211028
PL  - Brazil
TA  - Braz J Microbiol
JT  - Brazilian journal of microbiology : [publication of the Brazilian Society for 
      Microbiology]
JID - 101095924
SB  - IM
MH  - Animals
MH  - *Circovirus/genetics
MH  - Escherichia coli/genetics
MH  - Multiplex Polymerase Chain Reaction/methods
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Staphylococcus aureus/genetics
MH  - Swine
MH  - *Swine Diseases/diagnosis
PMC - PMC8882746
OTO - NOTNLM
OT  - Bacterium
OT  - Multiplex TaqMan qPCR
OT  - Virus
COIS- The authors declare no competing interests.
EDAT- 2021/10/29 06:00
MHDA- 2022/03/03 06:00
PMCR- 2022/10/28
CRDT- 2021/10/28 12:36
PHST- 2021/01/14 00:00 [received]
PHST- 2021/10/17 00:00 [accepted]
PHST- 2021/10/29 06:00 [pubmed]
PHST- 2022/03/03 06:00 [medline]
PHST- 2021/10/28 12:36 [entrez]
PHST- 2022/10/28 00:00 [pmc-release]
AID - 10.1007/s42770-021-00633-w [pii]
AID - 633 [pii]
AID - 10.1007/s42770-021-00633-w [doi]
PST - ppublish
SO  - Braz J Microbiol. 2022 Mar;53(1):359-368. doi: 10.1007/s42770-021-00633-w. Epub 
      2021 Oct 28.