PMID- 34710793
OWN - NLM
STAT- MEDLINE
DCOM- 20211214
LR  - 20211214
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 279
DP  - 2021 Dec
TI  - Human bone marrow-derived mesenchymal stem cells play a role as a vascular 
      pericyte in the reconstruction of human BBB on the angiogenesis microfluidic 
      chip.
PG  - 121210
LID - S0142-9612(21)00567-6 [pii]
LID - 10.1016/j.biomaterials.2021.121210 [doi]
AB  - A blood-brain barrier (BBB) on a chip similar to the in vivo BBB is important for 
      evaluating the efficacy of reparative cell therapeutics for ischemic stroke in 
      vitro. In this study, we established human BBB-like microvasculature on an 
      angiogenesis microfluidic chip and analyzed the role of human pericytes (hPCs) 
      and human astrocytes (hACs) on the architecture of human brain microvascular 
      endothelial cells (hBMEC)-derived microvasculature on a chip. We found that human 
      bone marrow mesenchymal stem cells (hBM-MSCs) play a role as perivascular 
      pericytes in tight BBB reformation with a better vessel-constrictive capacity 
      than that of hPCs, providing evidence of reparative stem cells on BBB repair 
      rather than a paracrine effect. We also demonstrated that pericytes play an 
      important role in vessel constriction, and astrocytes may induce the maturation 
      of a capillary network. Higher expression of VEGF, SDF-1alpha, PDGFRbeta, N-cadherin, 
      and alpha-SMA in hBM-MSCs than in hPCs and their subsequent downregulation with hBMEC 
      co-culture suggest that hBM-MSCs may be better recruited and engaged in the 
      BBB-microvasculature than hPCs. Collectively, the human BBB on a chip may be 
      adopted as an alternative to evaluate in vitro cellular behavior and the 
      engagement of cell therapeutics in BBB regeneration and may also be used for 
      studying stroke.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Kim, Sumin
AU  - Kim S
AD  - Department of Genetic Biotechnology, College of Life Science and Graduate School 
      of Biotechnology, Kyung Hee University, Yong in, 17104, South Korea.
FAU - Lee, Somin
AU  - Lee S
AD  - Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, 
      08826, South Korea.
FAU - Lim, Jungeun
AU  - Lim J
AD  - Department of Mechanical Engineering, Seoul National University, Seoul, 08826, 
      South Korea.
FAU - Choi, Hyeri
AU  - Choi H
AD  - Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, 
      08826, South Korea.
FAU - Kang, Habin
AU  - Kang H
AD  - Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, 
      08826, South Korea.
FAU - Jeon, Noo Li
AU  - Jeon NL
AD  - Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, 
      08826, South Korea; Department of Mechanical Engineering, Seoul National 
      University, Seoul, 08826, South Korea.
FAU - Son, Youngsook
AU  - Son Y
AD  - Department of Genetic Biotechnology, College of Life Science and Graduate School 
      of Biotechnology, Kyung Hee University, Yong in, 17104, South Korea; Kyung Hee 
      Institute of Regenerative Medicine (KIRM), Medical Science Research Institute, 
      Kyung Hee University Hospital, Seoul, 02447, South Korea. Electronic address: 
      ysson@khu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211021
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
SB  - IM
MH  - *Blood-Brain Barrier
MH  - Bone Marrow
MH  - Endothelial Cells
MH  - Humans
MH  - *Mesenchymal Stem Cells
MH  - Microfluidics
MH  - Pericytes
OTO - NOTNLM
OT  - BBB-On-a-chip
OT  - BM-MSC
OT  - Blood-brain barrier
OT  - Microfluidic chip
OT  - Pericyte
EDAT- 2021/10/29 06:00
MHDA- 2021/12/15 06:00
CRDT- 2021/10/28 20:27
PHST- 2021/01/06 00:00 [received]
PHST- 2021/10/07 00:00 [revised]
PHST- 2021/10/20 00:00 [accepted]
PHST- 2021/10/29 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/10/28 20:27 [entrez]
AID - S0142-9612(21)00567-6 [pii]
AID - 10.1016/j.biomaterials.2021.121210 [doi]
PST - ppublish
SO  - Biomaterials. 2021 Dec;279:121210. doi: 10.1016/j.biomaterials.2021.121210. Epub 
      2021 Oct 21.