PMID- 34711462
OWN - NLM
STAT- MEDLINE
DCOM- 20220414
LR  - 20220603
IS  - 1878-4380 (Electronic)
IS  - 0966-842X (Linking)
VI  - 30
IP  - 5
DP  - 2022 May
TI  - Adeno-associated virus (AAV) cell entry: structural insights.
PG  - 432-451
LID - S0966-842X(21)00216-X [pii]
LID - 10.1016/j.tim.2021.09.005 [doi]
AB  - Adeno-associated virus (AAV) is the leading vector in emerging treatments of 
      inherited diseases. Higher transduction efficiencies and cellular specificity are 
      required for broader clinical application, motivating investigations of 
      virus-host molecular interactions during cell entry. High-throughput methods are 
      identifying host proteins more comprehensively, with subsequent molecular studies 
      revealing unanticipated complexity and serotype specificity. Cryogenic electron 
      microscopy (cryo-EM) provides a path towards structural details of these 
      sometimes heterogeneous virus-host complexes, and is poised to illuminate more 
      fully the steps in entry. Here presented, is progress in understanding the 
      distinct steps of glycan attachment, and receptor-mediated entry/trafficking. 
      Comparison with structures of antibody complexes provides new insights on immune 
      neutralization with implications for the design of improved gene therapy vectors.
CI  - Copyright (c) 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Meyer, Nancy L
AU  - Meyer NL
AD  - Pacific Northwest Cryo-EM Center, Oregon Health and Science University (OHSU) 
      Center for Spatial Systems Biomedicine, Portland, OR, USA.
FAU - Chapman, Michael S
AU  - Chapman MS
AD  - Department of Biochemistry, University of Missouri, Columbia, MO 65211, USA. 
      Electronic address: chapmanms@missouri.edu.
LA  - eng
GR  - R35 GM122564/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Review
DEP - 20211025
PL  - England
TA  - Trends Microbiol
JT  - Trends in microbiology
JID - 9310916
RN  - 0 (Polysaccharides)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - *Dependovirus/genetics/metabolism
MH  - Genetic Vectors
MH  - Polysaccharides/metabolism
MH  - Receptors, Cell Surface/genetics
MH  - Serogroup
MH  - *Virus Internalization
OTO - NOTNLM
OT  - AAVR
OT  - antibody
OT  - attachment
OT  - gene therapy
OT  - glycan
OT  - receptor
COIS- Declaration of interests There are no interests to declare.
EDAT- 2021/10/30 06:00
MHDA- 2022/04/15 06:00
CRDT- 2021/10/29 05:44
PHST- 2021/06/16 00:00 [received]
PHST- 2021/09/02 00:00 [revised]
PHST- 2021/09/14 00:00 [accepted]
PHST- 2021/10/30 06:00 [pubmed]
PHST- 2022/04/15 06:00 [medline]
PHST- 2021/10/29 05:44 [entrez]
AID - S0966-842X(21)00216-X [pii]
AID - 10.1016/j.tim.2021.09.005 [doi]
PST - ppublish
SO  - Trends Microbiol. 2022 May;30(5):432-451. doi: 10.1016/j.tim.2021.09.005. Epub 
      2021 Oct 25.