PMID- 34713515
OWN - NLM
STAT- MEDLINE
DCOM- 20220404
LR  - 20220531
IS  - 1365-2826 (Electronic)
IS  - 0953-8194 (Linking)
VI  - 33
IP  - 11
DP  - 2021 Nov
TI  - Transgenic approaches to opening up new fields of vasopressin and oxytocin 
      research.
PG  - e13055
LID - 10.1111/jne.13055 [doi]
AB  - Transgenic approaches have been applied to generate transgenic rats that express 
      exogenous genes in arginine vasopressin (AVP)- and oxytocin (OXT)-producing 
      magnocellular neurosecretory cells (MNCs) of the hypothalamic-neurohypophyseal 
      system (HNS). First, the fusion gene that expresses AVP-enhanced green 
      fluorescent protein (eGFP) and OXT-monomeric red fluorescent protein 1 (mRFP1) 
      was used to visualize AVP- and OXT-producing MNCs and their axon terminals in the 
      HNS under fluorescence microscopy. Second, the fusion gene that expresses 
      c-fos-eGFP and c-fos-mRFP1 was used to identify activated neurons physiologically 
      in the central nervous system, including MNCs, circumventricular organs and 
      spinal cord. In addition, AVP-eGFP x c-fos-mRFP1 and OXT-mRFP1 x c-fos-eGFP 
      double transgenic rats were generated to identify activated AVP- and 
      OXT-producing MNCs using appropriate physiological stimuli. Third, the fusion 
      gene that expresses AVP-chanelrhodopsin 2 (ChR2)-eGFP and AVP-hM3Dq-mCherry was 
      used to activate AVP- and OXT-producing MNCs by optogenetic and chemogenetic 
      approaches. In each step, these transgenic approaches in rats have provided new 
      insights on the physiological roles of AVP and OXT not only in the HNS, but also 
      in the whole body. In this review, we summarize the transgenic rats that we 
      generated, as well as related physiological findings.
CI  - (c) 2021 British Society for Neuroendocrinology.
FAU - Ueta, Yoichi
AU  - Ueta Y
AUID- ORCID: 0000-0003-0702-9538
AD  - Department of Physiology, School of Medicine, University of Occupational and 
      Environmental Health, Kitakyushu, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20211028
PL  - United States
TA  - J Neuroendocrinol
JT  - Journal of neuroendocrinology
JID - 8913461
RN  - 113-79-1 (Arginine Vasopressin)
RN  - 50-56-6 (Oxytocin)
SB  - IM
MH  - Animals
MH  - *Arginine Vasopressin/genetics/metabolism
MH  - Hypothalamus/metabolism
MH  - Neurons/metabolism
MH  - *Oxytocin/metabolism
MH  - Rats
MH  - Rats, Transgenic
OTO - NOTNLM
OT  - c-fos
OT  - chemogenetics
OT  - fluorescent protein
OT  - optogenetics
OT  - oxytocin
OT  - rat
OT  - vasopressin
EDAT- 2021/10/30 06:00
MHDA- 2022/04/05 06:00
CRDT- 2021/10/29 06:46
PHST- 2021/10/07 00:00 [revised]
PHST- 2021/05/01 00:00 [received]
PHST- 2021/10/07 00:00 [accepted]
PHST- 2021/10/30 06:00 [pubmed]
PHST- 2022/04/05 06:00 [medline]
PHST- 2021/10/29 06:46 [entrez]
AID - 10.1111/jne.13055 [doi]
PST - ppublish
SO  - J Neuroendocrinol. 2021 Nov;33(11):e13055. doi: 10.1111/jne.13055. Epub 2021 Oct 
      28.