PMID- 34713984
OWN - NLM
STAT- MEDLINE
DCOM- 20231114
LR  - 20231129
IS  - 1932-8494 (Electronic)
IS  - 1932-8486 (Linking)
VI  - 306
IP  - 12
DP  - 2023 Dec
TI  - Electroacupuncture restores intestinal mucosal barrier through TLR4/NF-kappaB p65 
      pathway in functional dyspepsia-like rats.
PG  - 2927-2938
LID - 10.1002/ar.24800 [doi]
AB  - Functional dyspepsia (FD) is a common functional gastrointestinal disorder with 
      high morbidity. Electroacupuncture (EA) has been applied to treat FD for a long 
      time. The aim of this study was to investigate the effects of EA and its 
      mechanism about intestinal mucosal barrier in rodent model of FD. Male 
      Sprague-Dawley rats were randomly divided into the control group and the model 
      group. Then, the rats in model group were established to the FD model by 
      multifactor interventions. In Experiment 1, qualified FD-like rats were randomly 
      divided into three groups: FD, EA, and acupuncture (AP) groups. The interventions 
      of EA and AP lasted 14 days, food intake, and body weight were recorded every 
      5 days. In Experiment 2, qualified FD-like rats were randomly divided into five 
      groups: FD, EA, AP, EA + TAK242, and TAK242 groups. The interventions of EA and 
      AP lasted 14 days, while TAK242 injection continued for 6 days. The rats were 
      sacrificed for the measurement of serum Interleukin- 6 (IL-6) and Tumor necrosis 
      factor-alpha (TNF-alpha) assayed by ELISA. Western blotting was used to assess the 
      expression of TLR4, Myd88, NF-kappaB p65, p-NF-kappaB p65, TRAF6, ZO-1, and occludin in 
      the duodenum. The transmission electron microscope was used to observe the 
      ultrastructure of intestinal epithelial cells. Compared with the rats in the 
      group FD, the rats in EA group had significantly increase of body weight, food 
      intake, and protein expressions of ZO-1 and occludin, while expressions of TLR4, 
      Myd88, NF-kappaB p65, p-NF-kappaB p65, TRAF6 in the duodenum and IL-6, and TNF-alpha in serum 
      were decreased. The EA + TAK242 treatment had similar effects to the EA treatment 
      but with increased potency; compared with EA, AP showed similar but reduced 
      effects. Our data demonstrated that EA is more effective than AP in improving 
      intestine mucosal barrier. The possible mechanisms of EA may involve the 
      TLR4/NF-kappaB p65 pathway.
CI  - (c) 2021 American Association for Anatomy.
FAU - Wang, Dan
AU  - Wang D
AUID- ORCID: 0000-0003-1100-2928
AD  - Chongqing Medical University, College of Traditional Chinese Medicine, Chongqing, 
      China.
AD  - Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical 
      Sciences, Beijing, China.
AD  - Hubei University of Chinese Medicine, Wuhan, China.
FAU - Zhang, Jinling
AU  - Zhang J
AD  - Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical 
      Sciences, Beijing, China.
FAU - Yang, Daye
AU  - Yang D
AD  - Hubei University of Chinese Medicine, Wuhan, China.
FAU - Wang, Junying
AU  - Wang J
AD  - Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical 
      Sciences, Beijing, China.
FAU - Li, Jia
AU  - Li J
AD  - Hubei University of Chinese Medicine, Wuhan, China.
FAU - Han, Yongli
AU  - Han Y
AD  - Hubei University of Chinese Medicine, Wuhan, China.
FAU - Kang, Zhaoxia
AU  - Kang Z
AD  - Guizhou University of Traditional Chinese Medicine, Guiyang, China.
FAU - Zhang, Hongxing
AU  - Zhang H
AD  - Hubei University of Chinese Medicine, Wuhan, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211029
PL  - United States
TA  - Anat Rec (Hoboken)
JT  - Anatomical record (Hoboken, N.J. : 2007)
JID - 101292775
RN  - 0 (NF-kappa B)
RN  - 0 (ethyl 6-(N-(2-chloro-4-fluorophenyl)sulfamoyl)cyclohex-1-ene-1-carboxylate)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Interleukin-6)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Occludin)
RN  - 0 (TNF Receptor-Associated Factor 6)
SB  - IM
MH  - Rats
MH  - Male
MH  - Animals
MH  - NF-kappa B/metabolism
MH  - Rats, Sprague-Dawley
MH  - Toll-Like Receptor 4/metabolism
MH  - *Dyspepsia/therapy
MH  - *Electroacupuncture
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Interleukin-6/metabolism
MH  - Myeloid Differentiation Factor 88/metabolism
MH  - Occludin/metabolism
MH  - TNF Receptor-Associated Factor 6/metabolism
MH  - Body Weight
OTO - NOTNLM
OT  - TLR4 pathway
OT  - TLR4通路
OT  - electroacupuncture
OT  - functional dyspepsia
OT  - intestinal mucosal barrier
OT  - low-grade inflammation
OT  - 低度炎症
OT  - 功能性消化不良
OT  - 电针
OT  - 肠黏膜屏障
EDAT- 2021/10/30 06:00
MHDA- 2023/11/14 06:43
CRDT- 2021/10/29 09:04
PHST- 2021/09/06 00:00 [revised]
PHST- 2021/03/25 00:00 [received]
PHST- 2021/09/15 00:00 [accepted]
PHST- 2023/11/14 06:43 [medline]
PHST- 2021/10/30 06:00 [pubmed]
PHST- 2021/10/29 09:04 [entrez]
AID - 10.1002/ar.24800 [doi]
PST - ppublish
SO  - Anat Rec (Hoboken). 2023 Dec;306(12):2927-2938. doi: 10.1002/ar.24800. Epub 2021 
      Oct 29.