PMID- 34716580
OWN - NLM
STAT- MEDLINE
DCOM- 20220121
LR  - 20220121
IS  - 1095-8649 (Electronic)
IS  - 0022-1112 (Linking)
VI  - 100
IP  - 1
DP  - 2022 Jan
TI  - Insights into the functional role of grass carp IL-8 in head kidney leukocytes: 
      pro-inflammatory effects and signalling mechanisms.
PG  - 192-202
LID - 10.1111/jfb.14934 [doi]
AB  - Interleukin-8 (IL-8) is a critical chemokine regulating immune cells' chemotaxis 
      as well as their physiological or pathological activations. In fish cells, 
      recombinant IL-8 proteins induced transcriptions of pro-inflammatory cytokines. 
      Nonetheless, the exact mechanisms underlying the function of fish IL-8 as a 
      pro-inflammatory cytokine are still unclear. In this paper, the authors first 
      prepared recombinant grass carp IL-8 (rgcIL-8) using an Escherichia coli 
      expression system, and later confirmed rgcIL-8 increased gene expression of il8, 
      il1beta and tumour necrosis factor alpha (tnfalpha) in grass carp head kidney leukocytes 
      (HKLs). Using signalling pathway inhibitors, the authors showed that rgcIL-8 
      regulated transcriptions of pro-inflammatory cytokines via MAPK and/or NF-kappaB 
      signalling pathways. They cloned gcIL-8-specific receptor CXCR1 and subsequently 
      discovered that gcIL-8 could increase the activity of NF-kappaB and the transcription 
      of IL-1beta via CXCR1. Simultaneously, antibody neutralization assay showed that 
      endogenous IL-8 is partially relevant to the self-regulation of IL-1beta. Moreover, 
      rgcIL-8 led to the expression of inducible nitric oxide synthase gene, causing an 
      accumulation of nitric oxide in the culture medium of HKLs, suggesting the 
      potential of gcIL-8 to mediate inflammatory response. This study not only 
      enriched the function of IL-8 in teleost but also revealed it as a potential 
      target for the inflammatory control in grass carp.
CI  - (c) 2021 Fisheries Society of the British Isles.
FAU - Zhao, Minghui
AU  - Zhao M
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, China.
FAU - Liu, Yazhen
AU  - Liu Y
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, China.
FAU - Gao, Yajun
AU  - Gao Y
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, China.
FAU - Wang, Xinyan
AU  - Wang X
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, China.
FAU - Zhou, Hong
AU  - Zhou H
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, China.
FAU - Zhang, Anying
AU  - Zhang A
AUID- ORCID: 0000-0001-7318-5606
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, China.
LA  - eng
GR  - 2021YJ0277/applied basic research foundation of sichuan province/
PT  - Journal Article
DEP - 20211116
PL  - England
TA  - J Fish Biol
JT  - Journal of fish biology
JID - 0214055
RN  - 0 (Fish Proteins)
RN  - 0 (Interleukin-8)
SB  - IM
MH  - Animals
MH  - *Carps/genetics
MH  - *Fish Diseases
MH  - Fish Proteins
MH  - Head Kidney
MH  - Interleukin-8/genetics
MH  - Leukocytes
MH  - Signal Transduction
OTO - NOTNLM
OT  - CXCR1
OT  - MAPK
OT  - NF-kappaB
OT  - interleukin-8
OT  - nitric oxide product
EDAT- 2021/10/31 06:00
MHDA- 2022/01/22 06:00
CRDT- 2021/10/30 06:08
PHST- 2021/10/23 00:00 [revised]
PHST- 2021/07/28 00:00 [received]
PHST- 2021/10/26 00:00 [accepted]
PHST- 2021/10/31 06:00 [pubmed]
PHST- 2022/01/22 06:00 [medline]
PHST- 2021/10/30 06:08 [entrez]
AID - 10.1111/jfb.14934 [doi]
PST - ppublish
SO  - J Fish Biol. 2022 Jan;100(1):192-202. doi: 10.1111/jfb.14934. Epub 2021 Nov 16.