PMID- 34717055
OWN - NLM
STAT- MEDLINE
DCOM- 20220404
LR  - 20220531
IS  - 1619-3997 (Electronic)
IS  - 0300-5577 (Linking)
VI  - 50
IP  - 2
DP  - 2022 Feb 23
TI  - Maternal oxytocin administration modulates gene expression in the brains of 
      perinatal mice.
PG  - 207-218
LID - 10.1515/jpm-2020-0525 [doi]
AB  - OBJECTIVES: Oxytocin (OXT) is widely used to facilitate labor. However, little is 
      known about the effects of perinatal OXT exposure on the developing brain. We 
      investigated the effects of maternal OXT administration on gene expression in 
      perinatal mouse brains. METHODS: Pregnant C57BL/6 mice were treated with saline 
      or OXT at term (n=6-7/group). Dams and pups were euthanized on gestational day 
      (GD) 18.5 after delivery by C-section. Another set of dams was treated with 
      saline or OXT (n=6-7/group) and allowed to deliver naturally; pups were 
      euthanized on postnatal day 9 (PND9). Perinatal/neonatal brain gene expression 
      was determined using Illumina BeadChip Arrays and real time quantitative PCR. 
      Differential gene expression analyses were performed. In addition, the effect of 
      OXT on neurite outgrowth was assessed using PC12 cells. RESULTS: Distinct and 
      sex-specific gene expression patterns were identified in offspring brains 
      following maternal OXT administration at term. The microarray data showed that 
      female GD18.5 brains exhibited more differential changes in gene expression 
      compared to male GD18.5 brains. Specifically, Cnot4 and Frmd4a were significantly 
      reduced by OXT exposure in male and female GD18.5 brains, whereas Mtap1b, Srsf11, 
      and Syn2 were significantly reduced only in female GD18.5 brains. No significant 
      microarray differences were observed in PND9 brains. By quantitative PCR, OXT 
      exposure reduced Oxtr expression in female and male brains on GD18.5 and PND9, 
      respectively. PC12 cell differentiation assays revealed that OXT induced neurite 
      outgrowth. CONCLUSIONS: Prenatal OXT exposure induces sex-specific differential 
      regulation of several nervous system-related genes and pathways with important 
      neural functions in perinatal brains.
CI  - (c) 2021 Walter de Gruyter GmbH, Berlin/Boston.
FAU - Hsieh, Frances F
AU  - Hsieh FF
AD  - Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, 
      Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, 
      USA.
AD  - Division of Maternal-Fetal Medicine, Department of Obstetrics, Gynecology 
      Stamford Hospital, Stamford, CT, USA.
FAU - Korsunsky, Ilya
AU  - Korsunsky I
AUID- ORCID: 0000-0003-4848-3948
AD  - Institute of Molecular Medicine, Feinstein Institutes for Medical Research at 
      Northwell Health, Manhasset, NY, USA.
AD  - Division of Genetics, Department of Medicine at Brigham & Women's Hospital, 
      Harvard Medical School, Boston, MA, USA.
FAU - Shih, Andrew J
AU  - Shih AJ
AD  - Institute of Molecular Medicine, Feinstein Institutes for Medical Research at 
      Northwell Health, Manhasset, NY, USA.
FAU - Moss, Matthew A
AU  - Moss MA
AD  - Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, 
      USA.
FAU - Chatterjee, Prodyot K
AU  - Chatterjee PK
AD  - Institute of Molecular Medicine, Feinstein Institutes for Medical Research at 
      Northwell Health, Manhasset, NY, USA.
FAU - Deshpande, Jaai
AU  - Deshpande J
AD  - Institute of Molecular Medicine, Feinstein Institutes for Medical Research at 
      Northwell Health, Manhasset, NY, USA.
AD  - Providence Community Health Center, Providence, RI, USA.
FAU - Xue, Xiangying
AU  - Xue X
AD  - Institute of Molecular Medicine, Feinstein Institutes for Medical Research at 
      Northwell Health, Manhasset, NY, USA.
FAU - Madankumar, Swati
AU  - Madankumar S
AD  - Institute of Molecular Medicine, Feinstein Institutes for Medical Research at 
      Northwell Health, Manhasset, NY, USA.
FAU - Kumar, Gopal
AU  - Kumar G
AD  - Elmezzi Graduate School of Molecular Medicine at Northwell Health, Manhasset, NY, 
      USA.
FAU - Rochelson, Burton
AU  - Rochelson B
AD  - Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, 
      Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, 
      USA.
FAU - Metz, Christine N
AU  - Metz CN
AUID- ORCID: 0000-0002-1013-1691
AD  - Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, 
      Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, 
      USA.
AD  - Institute of Molecular Medicine, Feinstein Institutes for Medical Research at 
      Northwell Health, Manhasset, NY, USA.
AD  - Elmezzi Graduate School of Molecular Medicine at Northwell Health, Manhasset, NY, 
      USA.
LA  - eng
PT  - Journal Article
DEP - 20211101
PL  - Germany
TA  - J Perinat Med
JT  - Journal of perinatal medicine
JID - 0361031
RN  - 50-56-6 (Oxytocin)
SB  - IM
MH  - Animals
MH  - Brain/drug effects/metabolism
MH  - Female
MH  - Gene Expression/drug effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *Oxytocin/pharmacology
MH  - Pregnancy
OTO - NOTNLM
OT  - fetal brain
OT  - in utero exposure
OT  - neuritogenesis
OT  - oxytocin
OT  - parturition
EDAT- 2021/10/31 06:00
MHDA- 2022/04/05 06:00
CRDT- 2021/10/30 17:07
PHST- 2020/11/06 00:00 [received]
PHST- 2021/10/01 00:00 [accepted]
PHST- 2021/10/31 06:00 [pubmed]
PHST- 2022/04/05 06:00 [medline]
PHST- 2021/10/30 17:07 [entrez]
AID - jpm-2020-0525 [pii]
AID - 10.1515/jpm-2020-0525 [doi]
PST - epublish
SO  - J Perinat Med. 2021 Nov 1;50(2):207-218. doi: 10.1515/jpm-2020-0525. Print 2022 
      Feb 23.