PMID- 34718768
OWN - NLM
STAT- MEDLINE
DCOM- 20211227
LR  - 20211227
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Print)
IS  - 0305-1048 (Linking)
VI  - 49
IP  - 20
DP  - 2021 Nov 18
TI  - A post-transcriptional regulon controlled by TtpA, the single tristetraprolin 
      family member expressed in Dictyostelium discoideum.
PG  - 11920-11937
LID - 10.1093/nar/gkab983 [doi]
AB  - Post-transcriptional processes mediated by mRNA binding proteins represent 
      important control points in gene expression. In eukaryotes, mRNAs containing 
      specific AU-rich motifs are regulated by binding of tristetraprolin (TTP) family 
      tandem zinc finger proteins, which promote mRNA deadenylation and decay, partly 
      through interaction of a conserved C-terminal CNOT1 binding (CNB) domain with 
      CCR4-NOT protein complexes. The social ameba Dictyostelium discoideum shared a 
      common ancestor with humans more than a billion years ago, and expresses only one 
      TTP family protein, TtpA, in contrast to three members expressed in humans. 
      Evaluation of ttpA null-mutants identified six transcripts that were consistently 
      upregulated compared to WT during growth and early development. The 
      3'-untranslated regions (3'-UTRs) of all six 'TtpA-target' mRNAs contained 
      multiple TTP binding motifs (UUAUUUAUU), and one 3'-UTR conferred TtpA 
      post-transcriptional stability regulation to a heterologous mRNA that was 
      abrogated by mutations in the core TTP-binding motifs. All six target transcripts 
      were upregulated to similar extents in a C-terminal truncation mutant, in 
      contrast to less severe effects of analogous mutants in mice. All six target 
      transcripts encoded probable membrane proteins. In Dictyostelium, TtpA may 
      control an 'RNA regulon', where a single RNA binding protein, TtpA, 
      post-transcriptionally co-regulates expression of several functionally related 
      proteins.
CI  - Published by Oxford University Press on behalf of Nucleic Acids Research 2021.
FAU - Bai, Wenli
AU  - Bai W
AD  - Laboratory of Cellular and Developmental Biology, National Institute of Diabetes 
      and Digestive and Kidney Diseases, Bethesda, MD 20892, USA.
FAU - Wells, Melissa L
AU  - Wells ML
AD  - The Signal Transduction Laboratory, National Institute of Environmental Health 
      Sciences, Research Triangle Park, NC 27709, USA.
FAU - Lai, Wi S
AU  - Lai WS
AD  - The Signal Transduction Laboratory, National Institute of Environmental Health 
      Sciences, Research Triangle Park, NC 27709, USA.
FAU - Hicks, Stephanie N
AU  - Hicks SN
AD  - The Signal Transduction Laboratory, National Institute of Environmental Health 
      Sciences, Research Triangle Park, NC 27709, USA.
FAU - Burkholder, Adam B
AU  - Burkholder AB
AD  - Integrative Bioinformatics Support Group, National Institute of Environmental 
      Health Sciences, Research Triangle Park, NC 27709, USA.
FAU - Perera, Lalith
AU  - Perera L
AD  - Genome Integrity and Structural Biology Laboratory, National Institute of 
      Environmental Health Sciences, Research Triangle Park, NC 27709, USA.
FAU - Kimmel, Alan R
AU  - Kimmel AR
AD  - Laboratory of Cellular and Developmental Biology, National Institute of Diabetes 
      and Digestive and Kidney Diseases, Bethesda, MD 20892, USA.
FAU - Blackshear, Perry J
AU  - Blackshear PJ
AUID- ORCID: 0000-0002-9561-8529
AD  - The Signal Transduction Laboratory, National Institute of Environmental Health 
      Sciences, Research Triangle Park, NC 27709, USA.
AD  - The Departments of Medicine and Biochemistry, Duke University Medical Center, 
      Durham, NC 27710, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Protozoan Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tristetraprolin)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Dictyostelium/*genetics/metabolism
MH  - Mutation
MH  - Protozoan Proteins/genetics/*metabolism
MH  - RNA Stability
MH  - RNA, Messenger/chemistry/genetics/metabolism
MH  - *Regulon
MH  - Tristetraprolin/genetics/*metabolism
PMC - PMC8599788
EDAT- 2021/11/01 06:00
MHDA- 2021/12/28 06:00
PMCR- 2021/10/30
CRDT- 2021/10/31 21:15
PHST- 2021/10/12 00:00 [accepted]
PHST- 2021/10/05 00:00 [revised]
PHST- 2021/05/06 00:00 [received]
PHST- 2021/11/01 06:00 [pubmed]
PHST- 2021/12/28 06:00 [medline]
PHST- 2021/10/31 21:15 [entrez]
PHST- 2021/10/30 00:00 [pmc-release]
AID - 6414599 [pii]
AID - gkab983 [pii]
AID - 10.1093/nar/gkab983 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 2021 Nov 18;49(20):11920-11937. doi: 10.1093/nar/gkab983.