PMID- 34719315
OWN - NLM
STAT- MEDLINE
DCOM- 20220222
LR  - 20240220
IS  - 2165-5987 (Electronic)
IS  - 2165-5979 (Print)
IS  - 2165-5979 (Linking)
VI  - 13
IP  - 1
DP  - 2022 Jan
TI  - Dehydroevodiamine suppresses inflammatory responses in adjuvant-induced arthritis 
      rats and human fibroblast-like synoviocytes.
PG  - 268-279
LID - 10.1080/21655979.2021.1999554 [doi]
AB  - Dehydroevodiamine (DHE) is an effective natural active substance extracted from 
      Euodiae Fructus, which is a widely used herbal drug in traditional Chinese 
      medicine. The focus of this study was to test the possibility of using DHE in the 
      treatment of rheumatoid arthritis (RA) diseases. A rat model of adjuvant-induced 
      arthritis (AIA) was generated using Complete Freund's Adjuvant (CFA). Body weight 
      changes, arthritis scores, ankle pathology, tumor necrosis factor-alpha (TNF-alpha), 
      interleukin-1beta(IL-1beta), interleukin-6 (IL-6), and interleukin-17 (IL-17) 
      secretion, as well as matrix metalloproteinase (MMP) expression in joint tissue, 
      were measured as indicators of viability of DHE medicated AIA rats. Human 
      fibroblast-like synoviocytes (MH7A cells) were connected to check these impacts. 
      The results confirmed that DHE administration had an excellent therapeutic impact 
      on the AIA rat model, substantially relieving joint swelling, inhibiting synovial 
      pannus hyperplasia, and decreasing joint scores. In addition, the serum 
      enzyme-linked immunosorbent assay (ELISA) showed that DHE treatment reduced the 
      expression of pro-inflammatory factors in AIA rats. The immunohistochemical 
      results showed that DHE treatment could reduce the synthesis of MMPs such as 
      matrix metalloproteinase-1(MMP-1) and matrix metalloproteinase-3 (MMP-3) in the 
      ankle tissue of AIA rats. In vitro, DHE inhibited cell proliferation, mRNA 
      transcription, protein synthesis of proinflammatory factors such as IL-1betaand 
      IL-6, and matrix metalloproteinases such as MMP-1 and MMP-3. Furthermore, DHE 
      inhibited the phosphorylation levels of p38, JNK, and ERK proteins in 
      TNF-alpha-treated MH7A cells.This work assessed the effect of DHE in AIA rats and 
      revealed its mechanism in vitro.
FAU - Dai, Yufang
AU  - Dai Y
AD  - Department of Rheumatology and Immunology, Minda Hospital of Hubei Minzu 
      University, Hubei Provincial Key Laboratory of Occurrence and Intervention of 
      Rheumatic Disease, Enshi, China.
FAU - Sheng, Jiaoe
AU  - Sheng J
AD  - Department of Rheumatology and Immunology, Minda Hospital of Hubei Minzu 
      University, Hubei Provincial Key Laboratory of Occurrence and Intervention of 
      Rheumatic Disease, Enshi, China.
FAU - He, Sanshan
AU  - He S
AD  - Department of Rheumatology and Immunology, Minda Hospital of Hubei Minzu 
      University, Hubei Provincial Key Laboratory of Occurrence and Intervention of 
      Rheumatic Disease, Enshi, China.
FAU - Wu, Qingchao
AU  - Wu Q
AD  - Department of Rheumatology and Immunology, Minda Hospital of Hubei Minzu 
      University, Hubei Provincial Key Laboratory of Occurrence and Intervention of 
      Rheumatic Disease, Enshi, China.
FAU - Wang, Yunlong
AU  - Wang Y
AD  - Department of Rheumatology and Immunology, Minda Hospital of Hubei Minzu 
      University, Hubei Provincial Key Laboratory of Occurrence and Intervention of 
      Rheumatic Disease, Enshi, China.
FAU - Su, Linchong
AU  - Su L
AD  - Department of Rheumatology and Immunology, Minda Hospital of Hubei Minzu 
      University, Hubei Provincial Key Laboratory of Occurrence and Intervention of 
      Rheumatic Disease, Enshi, China.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
PL  - United States
TA  - Bioengineered
JT  - Bioengineered
JID - 101581063
RN  - 0 (Alkaloids)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 67909-49-3 (dehydroevodiamine)
RN  - 9007-81-2 (Freund's Adjuvant)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
RIN - Bioengineered. 2024 Dec;15(1):2299578. PMID: 38376393
MH  - Alkaloids/*administration & dosage/pharmacology
MH  - Animals
MH  - Anti-Inflammatory Agents/*administration & dosage/pharmacology
MH  - Arthritis, Experimental/chemically induced/*drug therapy/genetics/immunology
MH  - Body Weight/drug effects
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Cytokines/genetics/metabolism
MH  - Disease Models, Animal
MH  - Female
MH  - Freund's Adjuvant/*adverse effects
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Matrix Metalloproteinases/metabolism
MH  - Rats
MH  - Synoviocytes/*cytology/drug effects/immunology
PMC - PMC8805850
OTO - NOTNLM
OT  - Dehydroevodiamine
OT  - aia
OT  - mapk
OT  - mh7a
OT  - rheumatoid arthritis
COIS- The authors declare that there are no competing interests.
EDAT- 2021/11/02 06:00
MHDA- 2022/02/23 06:00
PMCR- 2021/12/27
CRDT- 2021/11/01 05:22
PHST- 2021/11/02 06:00 [pubmed]
PHST- 2022/02/23 06:00 [medline]
PHST- 2021/11/01 05:22 [entrez]
PHST- 2021/12/27 00:00 [pmc-release]
AID - 1999554 [pii]
AID - 10.1080/21655979.2021.1999554 [doi]
PST - ppublish
SO  - Bioengineered. 2022 Jan;13(1):268-279. doi: 10.1080/21655979.2021.1999554.