PMID- 34721522
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240403
IS  - 1664-8021 (Print)
IS  - 1664-8021 (Electronic)
IS  - 1664-8021 (Linking)
VI  - 12
DP  - 2021
TI  - Molecular Cytogenetics in the Era of Chromosomics and Cytogenomic Approaches.
PG  - 720507
LID - 10.3389/fgene.2021.720507 [doi]
LID - 720507
AB  - Here the role of molecular cytogenetics in the context of yet available all other 
      cytogenomic approaches is discussed. A short introduction how cytogenetics and 
      molecular cytogenetics were established is followed by technical aspects of 
      fluorescence in situ hybridization (FISH). The latter contains the methodology 
      itself, the types of probe- and target-DNA, as well as probe sets. The main part 
      deals with examples of modern FISH-applications, highlighting unique 
      possibilities of the approach, like the possibility to study individual cells and 
      even individual chromosomes. Different variants of FISH can be used to retrieve 
      information on genomes from (almost) base pair to whole genomic level, as besides 
      only second and third generation sequencing approaches can do. Here especially 
      highlighted variations of FISH are molecular combing, chromosome orientation-FISH 
      (CO-FISH), telomere-FISH, parental origin determination FISH (POD-FISH), FISH to 
      resolve the nuclear architecture, multicolor-FISH (mFISH) approaches, among other 
      applied in chromoanagenesis studies, Comet-FISH, and CRISPR-mediated 
      FISH-applications. Overall, molecular cytogenetics is far from being outdated and 
      actively involved in up-to-date diagnostics and research.
CI  - Copyright (c) 2021 Liehr.
FAU - Liehr, Thomas
AU  - Liehr T
AD  - Jena University Hospital, Institute of Human Genetics, Friedrich Schiller 
      University, Jena, Germany.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20211013
PL  - Switzerland
TA  - Front Genet
JT  - Frontiers in genetics
JID - 101560621
PMC - PMC8548727
OTO - NOTNLM
OT  - chromosomal heteromorphisms (CHMs)
OT  - chromosomics
OT  - chromothripsis
OT  - copy number variations (CNVs)
OT  - cytogenomics
OT  - glass-needle based chromosome microdissection (midi)
OT  - small supernumerary marker chromosomes (sSMCs)
OT  - topologically associating domains (TADs)
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/11/02 06:00
MHDA- 2021/11/02 06:01
PMCR- 2021/10/13
CRDT- 2021/11/01 09:12
PHST- 2021/06/04 00:00 [received]
PHST- 2021/09/22 00:00 [accepted]
PHST- 2021/11/01 09:12 [entrez]
PHST- 2021/11/02 06:00 [pubmed]
PHST- 2021/11/02 06:01 [medline]
PHST- 2021/10/13 00:00 [pmc-release]
AID - 720507 [pii]
AID - 10.3389/fgene.2021.720507 [doi]
PST - epublish
SO  - Front Genet. 2021 Oct 13;12:720507. doi: 10.3389/fgene.2021.720507. eCollection 
      2021.