PMID- 34721588
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211102
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2021
DP  - 2021
TI  - Human Umbilical Mesenchymal Stromal Cells Mixed with Hyaluronan Transplantation 
      Decreased Cartilage Destruction in a Rabbit Osteoarthritis Model.
PG  - 2989054
LID - 10.1155/2021/2989054 [doi]
LID - 2989054
AB  - Osteoarthritis (OA), the most common type of arthritis, causes pain in joints and 
      disability. Due to the absence of ideal effective medication, stem cell 
      transplantation emerges as a new hope for OA therapy. This study is aimed at 
      evaluating the capability of human umbilical cord mesenchymal stromal cells 
      (HUCMSCs) mixed with hyaluronan (HA) to treat osteoarthritis in a rabbit model. 
      Differentiation capability of HUCMSCs, magnetic resonance image examination, and 
      immunohistochemistry of the cartilage after transplantation of HUCMSCs mixed with 
      HA in a rabbit OA model were explored. HUCMSCs exhibited typical mesenchymal 
      stromal cell (MSC) characteristics, including spindle-shaped morphology, surface 
      marker expressions (positive for human leukocyte antigen- (HLA-) ABC, CD44, CD73, 
      CD90, and CD105; negative for HLA-DR, CD34, and CD45), and trilineage 
      differentiation (chondrogenesis, adipogenesis, and osteogenesis). The gene 
      expression of SOX9, type II collagen, and aggrecan in the HUCMSC-derived 
      chondrocytes mixed with HA was increased after in vitro chondrogenesis compared 
      with HUCMSCs. A gross and histological significant improvement in hyaline 
      cartilage destruction after HUCMSCs mixed with HA was noted in the animal model 
      compared to the OA knees. The International Cartilage Repair Society histological 
      score and Safranin O staining were significantly higher for the treated knees 
      than the control knees (p < 0.05). Moreover, the expression of MMP13 was 
      significantly decreased in the treated knees than in the OA knees. In conclusion, 
      HUCMSCs mixed with HA in vitro and in vivo might attenuate the cartilage 
      destruction in osteoarthritis. Our study provided evidence for future clinical 
      trials.
CI  - Copyright (c) 2021 Yu-Hsun Chang et al.
FAU - Chang, Yu-Hsun
AU  - Chang YH
AUID- ORCID: 0000-0001-7762-4469
AD  - Department of Pediatrics, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical 
      Foundation and Tzu Chi University, Hualien, Taiwan.
FAU - Ding, Dah-Ching
AU  - Ding DC
AUID- ORCID: 0000-0001-5105-068X
AD  - Department of Obstetrics and Gynecology, Hualien Tzu Chi Hospital, Buddhist Tzu 
      Chi Medical Foundation and Tzu Chi University, Hualien, Taiwan.
AD  - Department of Obstetrics and Gynecology, College of Medicine, Tzu Chi University, 
      Hualien, Taiwan.
FAU - Wu, Kun-Chi
AU  - Wu KC
AUID- ORCID: 0000-0001-6252-7957
AD  - Department of Orthopedics, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical 
      Foundation and Tzu Chi University, Hualien, Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20211021
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC8553511
COIS- The authors declare no conflict of interest.
EDAT- 2021/11/02 06:00
MHDA- 2021/11/02 06:01
PMCR- 2021/10/21
CRDT- 2021/11/01 09:13
PHST- 2021/07/09 00:00 [received]
PHST- 2021/09/14 00:00 [revised]
PHST- 2021/10/05 00:00 [accepted]
PHST- 2021/11/01 09:13 [entrez]
PHST- 2021/11/02 06:00 [pubmed]
PHST- 2021/11/02 06:01 [medline]
PHST- 2021/10/21 00:00 [pmc-release]
AID - 10.1155/2021/2989054 [doi]
PST - epublish
SO  - Stem Cells Int. 2021 Oct 21;2021:2989054. doi: 10.1155/2021/2989054. eCollection 
      2021.