PMID- 34722338
OWN - NLM
STAT- MEDLINE
DCOM- 20211124
LR  - 20211124
IS  - 2235-2988 (Electronic)
IS  - 2235-2988 (Linking)
VI  - 11
DP  - 2021
TI  - Leishmania Parasites Differently Regulate Antioxidant Genes in Macrophages 
      Derived From Resistant and Susceptible Mice.
PG  - 748738
LID - 10.3389/fcimb.2021.748738 [doi]
LID - 748738
AB  - Macrophage-Leishmania interactions are central to parasite growth and disease 
      outcome. Macrophages have developed various strategies to fight invaders, 
      including oxidative burst. While some microorganisms seem to survive and even 
      thrive in an oxidative environment, others are susceptible and get killed. To 
      counter oxidative stress, macrophages switch the expressions of cytoprotective 
      and detoxifying enzymes, which are downstream targets of the nuclear factor 
      erythroid 2-related factor 2 (Nrf2), to enhance cell survival. We have explored 
      the transcription of NRF2 and of its target genes and compared the effect of the 
      parasite on their transcription in bone marrow-derived macrophages (BMdMs) from 
      Leishmania-resistant and Leishmania-susceptible mice. While heme oxygenase 1 
      (HO-1) transcription is independent of the genetic background, the transcription 
      of glutathione reductase (Gsr) and of cysteine/glutamate exchange transporter 
      (Slc7a11), involved in glutathione accumulation, was differentially regulated in 
      BMdMs from both mouse strains. We also show that, except for HO-1, known to favor 
      the survival of the parasite, the transcription of the selected genes, including 
      Gsr, CD36, and catalase (CAT), was actively repressed, if not at all time points 
      at least at the later ones, by the parasite, especially in Balb/c BMdMs. 
      Consistent with these results, we found that the silencing of NRF2 in this study 
      increases the survival and multiplication of the parasite.
CI  - Copyright (c) 2021 Bichiou, Rabhi, Ben Hamda, Bouabid, Belghith, Piquemal, Trentin, 
      Rabhi and Guizani-Tabbane.
FAU - Bichiou, Haifa
AU  - Bichiou H
AD  - Laboratory of Medical Parasitology, Biotechnology and Biomolecules, Institut 
      Pasteur de Tunis, Tunis-Belvedere, Tunisia.
AD  - Faculty of Sciences of Tunis, Universite de Tunis El Manar, Tunis, Tunisia.
FAU - Rabhi, Sameh
AU  - Rabhi S
AD  - Laboratory of Medical Parasitology, Biotechnology and Biomolecules, Institut 
      Pasteur de Tunis, Tunis-Belvedere, Tunisia.
FAU - Ben Hamda, Cherif
AU  - Ben Hamda C
AD  - Laboratory of Medical Parasitology, Biotechnology and Biomolecules, Institut 
      Pasteur de Tunis, Tunis-Belvedere, Tunisia.
FAU - Bouabid, Cyrine
AU  - Bouabid C
AD  - Laboratory of Medical Parasitology, Biotechnology and Biomolecules, Institut 
      Pasteur de Tunis, Tunis-Belvedere, Tunisia.
AD  - Faculty of Sciences of Tunis, Universite de Tunis El Manar, Tunis, Tunisia.
FAU - Belghith, Meriam
AU  - Belghith M
AD  - Department of Immunology, Institut Pasteur de Tunis, University Tunis El-Manar, 
      Tunis, Tunisia.
FAU - Piquemal, David
AU  - Piquemal D
AD  - Acobiom, Grabels, France.
FAU - Trentin, Bernadette
AU  - Trentin B
AD  - Acobiom, Grabels, France.
FAU - Rabhi, Imen
AU  - Rabhi I
AD  - Laboratory of Medical Parasitology, Biotechnology and Biomolecules, Institut 
      Pasteur de Tunis, Tunis-Belvedere, Tunisia.
AD  - Higher Institute of Biotechnology at Sidi-Thabet, Biotechpole Sidi-Thabet, 
      University of Manouba, Sidi-Thabet, Tunisia.
FAU - Guizani-Tabbane, Lamia
AU  - Guizani-Tabbane L
AD  - Laboratory of Medical Parasitology, Biotechnology and Biomolecules, Institut 
      Pasteur de Tunis, Tunis-Belvedere, Tunisia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211015
PL  - Switzerland
TA  - Front Cell Infect Microbiol
JT  - Frontiers in cellular and infection microbiology
JID - 101585359
RN  - 0 (Antioxidants)
SB  - IM
MH  - Animals
MH  - Antioxidants
MH  - *Leishmania/genetics
MH  - Macrophages
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Oxidative Stress
MH  - *Parasites
PMC - PMC8554229
OTO - NOTNLM
OT  - Leishmania
OT  - NRF2
OT  - antioxidants
OT  - macrophage
OT  - resistance
OT  - susceptibility
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/11/02 06:00
MHDA- 2021/11/25 06:00
PMCR- 2021/01/01
CRDT- 2021/11/01 09:24
PHST- 2021/07/28 00:00 [received]
PHST- 2021/09/20 00:00 [accepted]
PHST- 2021/11/01 09:24 [entrez]
PHST- 2021/11/02 06:00 [pubmed]
PHST- 2021/11/25 06:00 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fcimb.2021.748738 [doi]
PST - epublish
SO  - Front Cell Infect Microbiol. 2021 Oct 15;11:748738. doi: 
      10.3389/fcimb.2021.748738. eCollection 2021.