PMID- 34723519
OWN - NLM
STAT- MEDLINE
DCOM- 20211111
LR  - 20211111
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 69
IP  - 44
DP  - 2021 Nov 10
TI  - Point Mutations in FgSdhC(2) or in the 5' Untranslated Region of FgSdhC(1) Confer 
      Resistance to a Novel Succinate Dehydrogenase Inhibitor Flubeneteram in Fusarium 
      graminearum.
PG  - 13006-13019
LID - 10.1021/acs.jafc.1c04363 [doi]
AB  - Fusarium graminearum is one of the phytopathogenic fungi causing cereal fusarium 
      head blight worldwide. Flubeneteram (Flu) is a novel succinate dehydrogenase 
      inhibitor (SDHI) which exhibits strong fungicidal activity against F. 
      graminearum. In this study, four Flu-resistant (Flu(R)) mutants were generated by 
      fungicide domestication from the wildtype strain PH-1. Sequencing alignment 
      results of FgSdh from PH-1 and Flu(R) mutants showed that all the mutations could 
      be categorized into three resistant genotypes. Genotype I had an A-to-T mutation 
      at the -57 bp of the 5' untranslated region (5'UTR) of FgSdhC(1), while genotypes 
      II and III carried nonsynonymous mutations conferring T77I or R86C in FgSdhC(2), 
      respectively. All the mutations conferring the Flu resistance and causing fitness 
      penalty were validated. The genotype I mutant showed high Flu-resistance, while 
      genotype II and III mutants exhibited low Flu resistance. Additionally, all the 
      Flu(R) genotypes showed distinct cross-resistance patterns among the five SDHIs.
FAU - Chen, Wenchan
AU  - Chen W
AUID- ORCID: 0000-0001-7882-1085
AD  - College of Plant Protection, Nanjing Agricultural University, Nanjing 210095 
      Jiangsu, China.
AD  - Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, 
      210095 Jiangsu, China.
FAU - Wei, Lingling
AU  - Wei L
AD  - College of Plant Protection, Nanjing Agricultural University, Nanjing 210095 
      Jiangsu, China.
FAU - Li, Xiujuan
AU  - Li X
AD  - College of Plant Protection, Nanjing Agricultural University, Nanjing 210095 
      Jiangsu, China.
FAU - Ma, Hongyu
AU  - Ma H
AD  - College of Plant Protection, Nanjing Agricultural University, Nanjing 210095 
      Jiangsu, China.
FAU - Lou, Tiancheng
AU  - Lou T
AD  - College of Plant Protection, Nanjing Agricultural University, Nanjing 210095 
      Jiangsu, China.
FAU - Zhang, Pengcheng
AU  - Zhang P
AD  - College of Plant Protection, Nanjing Agricultural University, Nanjing 210095 
      Jiangsu, China.
FAU - Zheng, Huanhuan
AU  - Zheng H
AD  - College of Plant Protection, Nanjing Agricultural University, Nanjing 210095 
      Jiangsu, China.
FAU - Zhu, Xiaolei
AU  - Zhu X
AUID- ORCID: 0000-0002-5672-5209
AD  - Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of 
      Chemistry, Central China Normal University, Wuhan 430079 Hubei, China.
FAU - Zhang, Yu
AU  - Zhang Y
AD  - College of Advanced Agricultural Sciences, Zhejiang A & F University, Hangzhou 
      311300, China.
FAU - Liu, Fengquan
AU  - Liu F
AUID- ORCID: 0000-0002-2029-0012
AD  - Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, 
      210095 Jiangsu, China.
FAU - Chen, Changjun
AU  - Chen C
AD  - College of Plant Protection, Nanjing Agricultural University, Nanjing 210095 
      Jiangsu, China.
FAU - Yang, Guangfu
AU  - Yang G
AUID- ORCID: 0000-0003-4384-2593
AD  - Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of 
      Chemistry, Central China Normal University, Wuhan 430079 Hubei, China.
LA  - eng
PT  - Journal Article
DEP - 20211101
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Fungicides, Industrial)
RN  - AB6MNQ6J6L (Succinic Acid)
RN  - EC 1.3.99.1 (Succinate Dehydrogenase)
RN  - Fusarium graminearum
SB  - IM
MH  - 5' Untranslated Regions
MH  - Drug Resistance, Fungal/genetics
MH  - *Fungicides, Industrial/pharmacology
MH  - *Fusarium/genetics
MH  - Plant Diseases
MH  - Point Mutation
MH  - Succinate Dehydrogenase/genetics
MH  - Succinic Acid
OTO - NOTNLM
OT  - 5'UTR
OT  - Fusarium graminearum
OT  - flubeneteram
OT  - mutations
OT  - resistance mechanism
EDAT- 2021/11/02 06:00
MHDA- 2021/11/12 06:00
CRDT- 2021/11/01 16:30
PHST- 2021/11/02 06:00 [pubmed]
PHST- 2021/11/12 06:00 [medline]
PHST- 2021/11/01 16:30 [entrez]
AID - 10.1021/acs.jafc.1c04363 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2021 Nov 10;69(44):13006-13019. doi: 10.1021/acs.jafc.1c04363. 
      Epub 2021 Nov 1.