PMID- 34723970
OWN - NLM
STAT- MEDLINE
DCOM- 20211203
LR  - 20211214
IS  - 1553-7404 (Electronic)
IS  - 1553-7390 (Print)
IS  - 1553-7390 (Linking)
VI  - 17
IP  - 11
DP  - 2021 Nov
TI  - Histone deacetylase 1 controls cardiomyocyte proliferation during embryonic heart 
      development and cardiac regeneration in zebrafish.
PG  - e1009890
LID - 10.1371/journal.pgen.1009890 [doi]
LID - e1009890
AB  - In contrast to mammals, the zebrafish maintains its cardiomyocyte proliferation 
      capacity throughout adulthood. However, neither the molecular mechanisms that 
      orchestrate the proliferation of cardiomyocytes during developmental heart growth 
      nor in the context of regeneration in the adult are sufficiently defined yet. We 
      identified in a forward genetic N-ethyl-N-nitrosourea (ENU) mutagenesis screen 
      the recessive, embryonic-lethal zebrafish mutant baldrian (bal), which shows 
      severely impaired developmental heart growth due to diminished cardiomyocyte 
      proliferation. By positional cloning, we identified a missense mutation in the 
      zebrafish histone deacetylase 1 (hdac1) gene leading to severe protein 
      instability and the loss of Hdac1 function in vivo. Hdac1 inhibition 
      significantly reduces cardiomyocyte proliferation, indicating a role of Hdac1 
      during developmental heart growth in zebrafish. To evaluate whether developmental 
      and regenerative Hdac1-associated mechanisms of cardiomyocyte proliferation are 
      conserved, we analyzed regenerative cardiomyocyte proliferation after Hdac1 
      inhibition at the wound border zone in cryoinjured adult zebrafish hearts and we 
      found that Hdac1 is also essential to orchestrate regenerative cardiomyocyte 
      proliferation in the adult vertebrate heart. In summary, our findings suggest an 
      important and conserved role of Histone deacetylase 1 (Hdac1) in developmental 
      and adult regenerative cardiomyocyte proliferation in the vertebrate heart.
FAU - Buhler, Anja
AU  - Buhler A
AUID- ORCID: 0000-0003-2927-2379
AD  - Molecular Cardiology, Department of Internal Medicine II, University of Ulm, Ulm, 
      Germany.
FAU - Gahr, Bernd M
AU  - Gahr BM
AUID- ORCID: 0000-0002-5755-6603
AD  - Molecular Cardiology, Department of Internal Medicine II, University of Ulm, Ulm, 
      Germany.
FAU - Park, Deung-Dae
AU  - Park DD
AUID- ORCID: 0000-0001-6265-4964
AD  - Molecular Cardiology, Department of Internal Medicine II, University of Ulm, Ulm, 
      Germany.
FAU - Bertozzi, Alberto
AU  - Bertozzi A
AUID- ORCID: 0000-0003-3091-1481
AD  - Institute of Biochemistry and Molecular Biology, University of Ulm, Ulm, Germany.
FAU - Boos, Alena
AU  - Boos A
AD  - Molecular Cardiology, Department of Internal Medicine II, University of Ulm, Ulm, 
      Germany.
FAU - Dalvoy, Mohankrishna
AU  - Dalvoy M
AUID- ORCID: 0000-0003-4430-4893
AD  - Institute of Biochemistry and Molecular Biology, University of Ulm, Ulm, Germany.
FAU - Pott, Alexander
AU  - Pott A
AD  - Molecular Cardiology, Department of Internal Medicine II, University of Ulm, Ulm, 
      Germany.
AD  - Department of Internal Medicine II, University of Ulm, Ulm, Germany.
FAU - Oswald, Franz
AU  - Oswald F
AD  - Department of Internal Medicine I, University of Ulm, Ulm, Germany.
FAU - Kovall, Rhett A
AU  - Kovall RA
AUID- ORCID: 0000-0003-0520-1613
AD  - Department of Molecular Genetics, Biochemistry and Microbiology, University of 
      Cincinnati College of Medicine, Cincinnati, Ohio, United States of America.
FAU - Kuhn, Bernhard
AU  - Kuhn B
AUID- ORCID: 0000-0002-1855-7072
AD  - Department of Pediatrics, University of Pittsburgh, and Richard King Mellon 
      Institute for Pediatric Research and Division of Pediatric Cardiology, Children's 
      Hospital of Pittsburgh of UPMC, Pittsburgh, Pennsylvania, United States of 
      America.
FAU - Weidinger, Gilbert
AU  - Weidinger G
AUID- ORCID: 0000-0003-3599-6760
AD  - Institute of Biochemistry and Molecular Biology, University of Ulm, Ulm, Germany.
FAU - Rottbauer, Wolfgang
AU  - Rottbauer W
AUID- ORCID: 0000-0003-4725-6217
AD  - Department of Internal Medicine II, University of Ulm, Ulm, Germany.
FAU - Just, Steffen
AU  - Just S
AUID- ORCID: 0000-0001-7396-1281
AD  - Molecular Cardiology, Department of Internal Medicine II, University of Ulm, Ulm, 
      Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211101
PL  - United States
TA  - PLoS Genet
JT  - PLoS genetics
JID - 101239074
RN  - 0 (Zebrafish Proteins)
RN  - EC 3.5.1.98 (HDAC1 protein, zebrafish)
RN  - EC 3.5.1.98 (Histone Deacetylase 1)
SB  - IM
MH  - Animals
MH  - Cell Proliferation
MH  - Heart/*physiology
MH  - Histone Deacetylase 1/*metabolism
MH  - Myocytes, Cardiac/*cytology
MH  - Regeneration/*physiology
MH  - Zebrafish/*embryology
MH  - Zebrafish Proteins/*metabolism
PMC - PMC8584950
COIS- The authors have declared that no competing interests exist.
EDAT- 2021/11/02 06:00
MHDA- 2021/12/15 06:00
PMCR- 2021/11/01
CRDT- 2021/11/01 16:45
PHST- 2020/11/11 00:00 [received]
PHST- 2021/10/18 00:00 [accepted]
PHST- 2021/11/11 00:00 [revised]
PHST- 2021/11/02 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/11/01 16:45 [entrez]
PHST- 2021/11/01 00:00 [pmc-release]
AID - PGENETICS-D-20-01716 [pii]
AID - 10.1371/journal.pgen.1009890 [doi]
PST - epublish
SO  - PLoS Genet. 2021 Nov 1;17(11):e1009890. doi: 10.1371/journal.pgen.1009890. 
      eCollection 2021 Nov.