PMID- 34727112
OWN - NLM
STAT- MEDLINE
DCOM- 20211229
LR  - 20211229
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 16
IP  - 11
DP  - 2021
TI  - Mass recovery following caloric restriction reverses lipolysis and proteolysis, 
      but not gluconeogenesis, in insulin resistant OLETF rats.
PG  - e0252360
LID - 10.1371/journal.pone.0252360 [doi]
LID - e0252360
AB  - Caloric restriction (CR) is one of the most important behavioral interventions to 
      reduce excessive abdominal adiposity, which is a risk factor for the development 
      of insulin resistance. Previous metabolomics studies have characterized substrate 
      metabolism during healthy conditions; however, the effects of CR and subsequent 
      mass recovery on shifts in substrate metabolism during insulin resistance (IR) 
      have not been widely investigated. To assess the effects of acute CR and the 
      subsequent mass recovery on shifts in substrate metabolism, a cohort of 15-week 
      old Long Evans Tokushima Otsuka (LETO) and Otsuka Long Evans Tokushima Fatty 
      (OLETF) rats were calorie restricted (CR: 50% x 10 days) with or without partial 
      body mass recovery (PR; 73% x 7 days), along with their respective ad libitum 
      controls. End-of-study plasma samples were analyzed for primary carbon 
      metabolites by gas chromatography (GC) time-of-flight (TOF) mass spectrometry 
      (MS) data acquisition. Data analysis included PCA, Pearson correlation vs 
      previously reported variables (adipose and body masses, and insulin resistance 
      index, IRI), and metabolomics maps (MetaMapp) generated for the most significant 
      group comparisons. All treatments elicited a significant group differentiation in 
      at least one principal component. CR improved TCA cycle in OLETF, and increased 
      lipolysis and proteolysis. These changes were reversed after PR except for 
      gluconeogenesis. Plasma lipid concentrations were inversely correlated to IRI in 
      LETO, but not OLETF. These shifts in substrate metabolism suggest that the 
      CR-induced decreases in adipose may not be sufficient to more permanently alter 
      substrate metabolism to improve IR status during metabolic syndrome.
FAU - Cornejo, Manuel A
AU  - Cornejo MA
AUID- ORCID: 0000-0002-2446-8674
AD  - School of Natural Sciences, University of California, Merced, CA, United States 
      of America.
FAU - Dhillon, Jaapna
AU  - Dhillon J
AUID- ORCID: 0000-0003-4798-9111
AD  - Department of Nutrition and Exercise Physiology, University of Missouri, 
      Columbia, MO, United States of America.
FAU - Nishiyama, Akira
AU  - Nishiyama A
AD  - Department of Pharmacology, Faculty of Medicine, Kagawa University, Kagawa, 
      Japan.
FAU - Nakano, Daisuke
AU  - Nakano D
AD  - Department of Pharmacology, Faculty of Medicine, Kagawa University, Kagawa, 
      Japan.
FAU - Ortiz, Rudy M
AU  - Ortiz RM
AD  - School of Natural Sciences, University of California, Merced, CA, United States 
      of America.
LA  - eng
SI  - figshare/10.6084/m9.figshare.12831005
GR  - T37 MD001480/MD/NIMHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20211102
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Adipose Tissue/metabolism
MH  - Animals
MH  - *Caloric Restriction
MH  - Gluconeogenesis/*physiology
MH  - Glucose Tolerance Test
MH  - Insulin Resistance/*physiology
MH  - Lipolysis/*physiology
MH  - Liver/*metabolism
MH  - Proteolysis
MH  - Rats
MH  - Rats, Inbred OLETF
PMC - PMC8562784
COIS- The authors have declared that no competing interests exist.
EDAT- 2021/11/03 06:00
MHDA- 2021/12/30 06:00
PMCR- 2021/11/02
CRDT- 2021/11/02 17:19
PHST- 2021/05/19 00:00 [received]
PHST- 2021/10/18 00:00 [accepted]
PHST- 2021/11/02 17:19 [entrez]
PHST- 2021/11/03 06:00 [pubmed]
PHST- 2021/12/30 06:00 [medline]
PHST- 2021/11/02 00:00 [pmc-release]
AID - PONE-D-21-11012 [pii]
AID - 10.1371/journal.pone.0252360 [doi]
PST - epublish
SO  - PLoS One. 2021 Nov 2;16(11):e0252360. doi: 10.1371/journal.pone.0252360. 
      eCollection 2021.