PMID- 34729107
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211105
IS  - 1837-9664 (Print)
IS  - 1837-9664 (Electronic)
IS  - 1837-9664 (Linking)
VI  - 12
IP  - 23
DP  - 2021
TI  - Anticancer Effects of Helminthostachys zeylanica Ethyl acetate Extracts on Human 
      Gastric Cancer Cells through Downregulation of the TNF-alpha-activated 
      COX-2-cPLA2-PGE(2) Pathway.
PG  - 7052-7068
LID - 10.7150/jca.64638 [doi]
AB  - Background: Gastric cancer (GC) is the second most prevalent cancer worldwide and 
      the eighth most common cause of tumor-related death in Taiwan. Helminthostachys 
      zeylanica, a flavonoid compound, has anti-inflammatory, immunomodulatory, and 
      anticancer effects. We examined whether an extract of H. zeylanica (E1 and E2) 
      has potential as a treatment for GC. Methods: We investigated the effects 
      (pro-apoptosis, pro-autophagy, and antiproliferation ability) of H. zeylanica-E2 
      on cell viability in healthy gastric epithelial (GES-1) and GC cells (AGS and 
      BGC823). H. zeylanica-E2 was toxic to GC cells but had little or no toxicity to 
      normal cells. Results: In this study, H. zeylanica-E2 induced apoptosis through 
      caspase 3/7, Bcl-2, Bax, cyclooxygenase-2 (COX-2), and cleaved poly (ADP-ribose) 
      polymerase pathways in GC cells. In addition, it increased autophagy by 
      stimulating autophagy-related protein (ATG)5, ATG7, LC3-I/LC3-II, and inhibiting 
      COX-2 activity in GC cells. We also found that H. zeylanica-E2 exhibited 
      antiproliferation ability through cell cycle arrest in G0/G1 and G2/M and 
      suppressed the migration of GC cells. The anticancer effects of H. zeylanica-E2 
      in GC cells might be mediated partly through inhibition of tumor necrosis 
      factor-alpha (TNF-alpha)-activated proinflammatory cytosolic phospholipase A2 
      (cPLA2)-COX-2-prostaglandin E(2) (PGE(2)) pathway. Conclusions: Our results 
      suggest that H. zeylanica-E2 has potential as a novel adjunctive agent for the 
      treatment of GC.
CI  - (c) The author(s).
FAU - Tsai, Ming-Ming
AU  - Tsai MM
AD  - Department of Nursing, Division of Basic Medical Sciences, Research Center for 
      Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of 
      Science and Technology, Taoyuan, Taiwan and Department of General Surgery, Chang 
      Gung Memorial Hospital at Chiayi, Chiayi, Taiwan.
FAU - Lin, Horng-Chyuan
AU  - Lin HC
AD  - Department of Thoracic Medicine, Chang Gung Memorial Hospital at Linkou and 
      College of Medicine, Chang Gung University, Taoyuan, Taiwan.
FAU - Yu, Ming-Chin
AU  - Yu MC
AD  - Department of Surgery, New Taipei Municipal TuCheng Hospital, Chang Gung Memorial 
      Hospital at Linkou, and College of Medicine, Chang Gung University, Taoyuan, 
      Taiwan.
FAU - Lin, Wan-Jung
AU  - Lin WJ
AD  - Graduate Institute of Health Industry Technology, Research Center for Chinese 
      Herbal Medicine and Research Center for Food and Cosmetic Safety, College of 
      Human Ecology, Chang Gung University of Science and Technology, Taoyuan, Taiwan.
FAU - Chu, Mei-Yi
AU  - Chu MY
AD  - Graduate Institute of Health Industry Technology, Research Center for Chinese 
      Herbal Medicine and Research Center for Food and Cosmetic Safety, College of 
      Human Ecology, Chang Gung University of Science and Technology, Taoyuan, Taiwan.
FAU - Tsai, Ching-Ching
AU  - Tsai CC
AD  - Department of Nursing, College of Nursing, Chang Gung University of Science and 
      Technology, and Department of Cardiology, Chang Gung Memorial Hospital at Linkou, 
      Taoyuan, Taiwan.
FAU - Cheng, Ching-Yi
AU  - Cheng CY
AD  - Graduate Institute of Health Industry Technology, Research Center for Chinese 
      Herbal Medicine and Research Center for Food and Cosmetic Safety, College of 
      Human Ecology, Chang Gung University of Science and Technology, Taoyuan, Taiwan.
AD  - Department of Pulmonary Infection and Immunology, Chang Gung Memorial Hospital at 
      Linkou, Taoyuan, Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20211017
PL  - Australia
TA  - J Cancer
JT  - Journal of Cancer
JID - 101535920
PMC - PMC8558661
OTO - NOTNLM
OT  - Helminthostachys zeylanica
OT  - anti-inflammation
OT  - anticancer
OT  - gastric cancer
COIS- Competing Interests: The authors have declared that no competing interest exists.
EDAT- 2021/11/04 06:00
MHDA- 2021/11/04 06:01
PMCR- 2021/01/01
CRDT- 2021/11/03 06:56
PHST- 2021/07/06 00:00 [received]
PHST- 2021/09/24 00:00 [accepted]
PHST- 2021/11/03 06:56 [entrez]
PHST- 2021/11/04 06:00 [pubmed]
PHST- 2021/11/04 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - jcav12p7052 [pii]
AID - 10.7150/jca.64638 [doi]
PST - epublish
SO  - J Cancer. 2021 Oct 17;12(23):7052-7068. doi: 10.7150/jca.64638. eCollection 2021.