PMID- 34729639
OWN - NLM
STAT- MEDLINE
DCOM- 20220218
LR  - 20220301
IS  - 1435-702X (Electronic)
IS  - 0721-832X (Print)
IS  - 0721-832X (Linking)
VI  - 260
IP  - 3
DP  - 2022 Mar
TI  - Cell composition at the vitreomacular interface in traumatic macular holes.
PG  - 873-884
LID - 10.1007/s00417-021-05470-z [doi]
AB  - PURPOSE: To describe characteristics of the vitreomacular interface (VMI) in 
      traumatic macular holes (TMH) compared to idiopathic macular holes (IMH) using 
      immunofluorescence and electron microscopy, and to correlate with clinical data. 
      METHODS: For immunocytochemical and ultrastructural analyses, premacular tissue 
      with internal limiting membrane (ILM) and epiretinal membrane (ERM) was harvested 
      during vitrectomy from 5 eyes with TMH and 5 eyes with IMH. All specimens were 
      processed as flat mounts for phase-contrast microscopy, interference and 
      fluorescence microscopy, and transmission electron microscopy (TEM). Primary 
      antibodies were used against microglial and macroglial cells. Clinical data was 
      retrospectively evaluated. RESULTS: Surgically excised premacular tissue of eyes 
      with TMH showed a less pronounced positive immunoreactivity for anti-glutamine 
      synthetase, anti-vimentin and anti-IBA1 compared to eyes with IMH. Cell nuclei 
      staining of the flat-mounted specimens as well as TEM presented a lower cell 
      count in eyes with TMH compared to IMH. All detected cells were found on the 
      vitreal side of the ILM. No collagen fibrils were seen in specimens of TMH. 
      According to patients' age, intraoperative data as well as spectral-domain 
      optical coherence tomography (SD-OCT) analysis revealed an attached posterior 
      vitreous in the majority of TMH cases (60%), whereas all eyes with IMH presented 
      posterior vitreous detachment. CONCLUSION: The vitreomacular interface in TMH and 
      IMH shows significant differences. In TMH, glial cells are a rare finding on the 
      vitreal side of the ILM.
CI  - (c) 2021. The Author(s).
FAU - Guenther, Stefanie R
AU  - Guenther SR
AUID- ORCID: 0000-0001-8855-9308
AD  - Department of Ophthalmology, Ludwig-Maximilians-University, Vitreoretinal 
      Pathology Unit, Mathildenstrasse 8, 80336, Munich, Germany. 
      stefanie.guenther@med.uni-muenchen.de.
FAU - Schumann, Ricarda G
AU  - Schumann RG
AD  - Munich Eye Center Brienner Hof, Munich, Germany.
FAU - Zaytseva, Yulia
AU  - Zaytseva Y
AD  - Department of Ophthalmology, Ludwig-Maximilians-University, Vitreoretinal 
      Pathology Unit, Mathildenstrasse 8, 80336, Munich, Germany.
FAU - Hagenau, Felix
AU  - Hagenau F
AD  - Department of Ophthalmology, Ludwig-Maximilians-University, Vitreoretinal 
      Pathology Unit, Mathildenstrasse 8, 80336, Munich, Germany.
FAU - Wolf, Armin
AU  - Wolf A
AD  - Department of Ophthalmology, University of Ulm, Ulm, Germany.
FAU - Priglinger, Siegfried G
AU  - Priglinger SG
AD  - Department of Ophthalmology, Ludwig-Maximilians-University, Vitreoretinal 
      Pathology Unit, Mathildenstrasse 8, 80336, Munich, Germany.
FAU - Vogt, Denise
AU  - Vogt D
AD  - Department of Ophthalmology, Ludwig-Maximilians-University, Vitreoretinal 
      Pathology Unit, Mathildenstrasse 8, 80336, Munich, Germany.
LA  - eng
PT  - Journal Article
DEP - 20211103
PL  - Germany
TA  - Graefes Arch Clin Exp Ophthalmol
JT  - Graefe's archive for clinical and experimental ophthalmology = Albrecht von 
      Graefes Archiv fur klinische und experimentelle Ophthalmologie
JID - 8205248
SB  - IM
MH  - Basement Membrane/metabolism
MH  - *Epiretinal Membrane/diagnosis/metabolism/surgery
MH  - Humans
MH  - *Retinal Perforations/diagnosis/metabolism/surgery
MH  - Retrospective Studies
MH  - Tomography, Optical Coherence/methods
MH  - Vitrectomy/methods
PMC - PMC8850235
OTO - NOTNLM
OT  - Immunocytochemistry
OT  - Internal limiting membrane
OT  - Membrane peeling
OT  - Transmission electron microscopy
OT  - Traumatic macular hole
OT  - Vitreomacular interface
COIS- The authors declare no competing interests.
EDAT- 2021/11/04 06:00
MHDA- 2022/02/19 06:00
PMCR- 2021/11/03
CRDT- 2021/11/03 07:05
PHST- 2021/03/11 00:00 [received]
PHST- 2021/10/16 00:00 [accepted]
PHST- 2021/09/27 00:00 [revised]
PHST- 2021/11/04 06:00 [pubmed]
PHST- 2022/02/19 06:00 [medline]
PHST- 2021/11/03 07:05 [entrez]
PHST- 2021/11/03 00:00 [pmc-release]
AID - 10.1007/s00417-021-05470-z [pii]
AID - 5470 [pii]
AID - 10.1007/s00417-021-05470-z [doi]
PST - ppublish
SO  - Graefes Arch Clin Exp Ophthalmol. 2022 Mar;260(3):873-884. doi: 
      10.1007/s00417-021-05470-z. Epub 2021 Nov 3.