PMID- 34733331
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211105
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2021
DP  - 2021
TI  - Activated Human Umbilical Cord Blood Platelet-Rich Plasma Enhances the Beneficial 
      Effects of Human Umbilical Cord Mesenchymal Stem Cells in Chemotherapy-Induced 
      POF Rats.
PG  - 8293699
LID - 10.1155/2021/8293699 [doi]
LID - 8293699
AB  - Saving the ovarian function of premature ovarian failure (POF) patients 
      undergoing chemotherapy is an important problem in the field of reproductive 
      medicine. At present, human umbilical cord mesenchymal stem cells (HucMSCs) have 
      been used in the treatment of POF, but the effect is still not optimal. The 
      purpose of this study was to determine whether human umbilical cord blood 
      platelet-rich plasma (ucPRP) enhances the beneficial effects of HucMSCs in the 
      treatment of POF. First, we observed the effects of changes in the biological 
      activity of ucPRP on HucMSCs in vitro. Subsequently, we tracked the distribution 
      and function of the HucMSCs in POF rats, and the rats' estrus cycle and serum sex 
      hormones, follicular development, ovarian angiogenesis, ovarian granulosa cell 
      proliferation, and apoptosis were assessed. The results of the study showed that 
      the addition of ucPRP in vitro accelerates proliferation and reduces apoptosis of 
      the HucMSCs while upregulating the stemness gene of the HucMSCs. The combined 
      transplantation of HucMSCs and ucPRP resulted in more stem cells being retained 
      in the ovaries of POF rats, the estrus cycle of the POF rats being restored, the 
      levels of serum E2, AMH, and FSH improving, and damaged follicles beginning to 
      grow. Finally, we confirmed that the potential mechanism of the combination of 
      HucMSCs and ucPRP to rescue the ovarian function of POF rats is to promote 
      ovarian angiogenesis and to promote the proliferation and reduce the apoptosis of 
      ovarian granulosa cells. The upregulation of AMH and FHSR expression and the 
      downregulation of caspase-3 expression in granulosa cells are potential 
      mechanisms for the recovery of ovarian function. Our research results suggest 
      that the combined application of HucMSCs and ucPRP is a safe and efficient 
      transplantation program for the treatment of POF, thus providing a reliable 
      experimental basis for the clinical application of stem cell therapy in POF.
CI  - Copyright (c) 2021 Jiawei Wang et al.
FAU - Wang, Jiawei
AU  - Wang J
AUID- ORCID: 0000-0002-2692-299X
AD  - Center of Reproductive Medicine, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning 530000, China.
FAU - Zhao, Yunxiao
AU  - Zhao Y
AD  - Center of Reproductive Medicine, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning 530000, China.
FAU - Zheng, Fengque
AU  - Zheng F
AD  - Center of Reproductive Medicine, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning 530000, China.
FAU - Ma, Nana
AU  - Ma N
AD  - Center of Reproductive Medicine, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning 530000, China.
FAU - Qin, Rongyan
AU  - Qin R
AD  - Center of Reproductive Medicine, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning 530000, China.
FAU - Qin, Weili
AU  - Qin W
AD  - Center of Reproductive Medicine, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning 530000, China.
FAU - Liu, Bo
AU  - Liu B
AUID- ORCID: 0000-0002-0536-6197
AD  - Center of Reproductive Medicine, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning 530000, China.
FAU - Qin, Aiping
AU  - Qin A
AUID- ORCID: 0000-0002-0185-7204
AD  - Center of Reproductive Medicine, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning 530000, China.
LA  - eng
PT  - Journal Article
DEP - 20211025
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC8560297
COIS- The authors declare that they have no competing interests.
EDAT- 2021/11/05 06:00
MHDA- 2021/11/05 06:01
PMCR- 2021/10/25
CRDT- 2021/11/04 06:23
PHST- 2021/04/22 00:00 [received]
PHST- 2021/08/29 00:00 [revised]
PHST- 2021/09/20 00:00 [accepted]
PHST- 2021/11/04 06:23 [entrez]
PHST- 2021/11/05 06:00 [pubmed]
PHST- 2021/11/05 06:01 [medline]
PHST- 2021/10/25 00:00 [pmc-release]
AID - 10.1155/2021/8293699 [doi]
PST - epublish
SO  - Stem Cells Int. 2021 Oct 25;2021:8293699. doi: 10.1155/2021/8293699. eCollection 
      2021.