PMID- 34736163
OWN - NLM
STAT- MEDLINE
DCOM- 20220301
LR  - 20240226
IS  - 1532-3072 (Electronic)
IS  - 0040-8166 (Linking)
VI  - 73
DP  - 2021 Dec
TI  - MiR-552-3p facilitated cell proliferation, migration and invasion by sponging 
      Fibulin 5 in non-small cell lung cancer via activation of ERK/GSK3beta/beta-catenin 
      signaling pathway.
PG  - 101672
LID - S0040-8166(21)00188-9 [pii]
LID - 10.1016/j.tice.2021.101672 [doi]
AB  - Apart from the fact that miR-552-3p is known to promote cell progression among 
      various cancers, its function on non-small cell lung cancer (NSCLC) is unknown 
      which therefore emerges as the purpose of this research. TargetScan, Starbase, 
      miRWalk, miRDB and the Cancer Genome Atlas Lung Adenocarcinoma (TCGA-LUAD) were 
      utilized to analyze the target genes of miR-552-3p. NSCLC cells were transfected 
      with miR-552-3p mimic, miR-552-3p inhibitor, Fibulin 5 (FBLN5) overexpression 
      plasmid, and small interfering FBLN5 (siFBLN5) and treated with extracellular 
      regulated protein kinases (ERK) pathway inhibitor PD98059. MiR-552-3p, FBLN5, 
      p-ERK, ERK, p-glycogen synthase kinase 3beta (GSK3beta) and beta-catenin levels were 
      detected through quantitative reverse transcription-polymerase chain reaction and 
      western blot. The binding sites between miR-552-3p and FBLN5 were predicted by 
      TargetScan, which was tested through dual luciferase reporter analysis. Cell 
      viability, migration and invasion were determined by cell counting kit-8 (CCK-8) 
      assay, wound healing assay and transwell assay, respectively. MiR-552-3p 
      expression was upregulated in NSCLC and FBLN5 functioned as its target. 
      MiR-552-3p mimic promoted proliferation, migration, invasion, p-ERK, p-GSK3beta and 
      beta-catenin expressions in NSCLC cells while miR-552-3p inhibitor did the opposite. 
      Overexpressed FBLN5 suppressed proliferation, migration, invasion, p-ERK, p-GSK3beta 
      and beta-catenin expressions in NSCLC cells whereas siFBLN5 exerted the effects 
      opposite to overexpressed FBLN5. PD98059 enhanced the effect of overexpressed 
      FBLN5 on NSCLC cell migration and invasion while reversing the effect of siFBLN5. 
      MiR-552-3p facilitated cell proliferation, migration and invasion in NSCLC 
      through sponging FBLN5 via activation of ERK/GSK3beta/beta-catenin pathway.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Huang, Mingfang
AU  - Huang M
AD  - Thoracic Surgery Department, Hainan General Hospital, China.
FAU - Liao, Xuqiang
AU  - Liao X
AD  - Thoracic Surgery Department, Hainan General Hospital, China.
FAU - Li, Liang
AU  - Li L
AD  - Thoracic Surgery Department, Hainan General Hospital, China.
FAU - Li, Gao
AU  - Li G
AD  - Thoracic Surgery Department, Hainan General Hospital, China.
FAU - Chen, Minbiao
AU  - Chen M
AD  - Thoracic Surgery Department, Hainan General Hospital, China. Electronic address: 
      chenminbiao_cmb1@163.com.
LA  - eng
PT  - Journal Article
DEP - 20211029
PL  - Scotland
TA  - Tissue Cell
JT  - Tissue & cell
JID - 0214745
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (FBLN5 protein, human)
RN  - 0 (MIRN552 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (beta Catenin)
RN  - EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Carcinoma, Non-Small-Cell Lung/*genetics/pathology
MH  - Cell Line
MH  - Cell Movement/*genetics
MH  - Cell Proliferation/genetics
MH  - Extracellular Matrix Proteins/genetics/*metabolism
MH  - Extracellular Signal-Regulated MAP Kinases/*metabolism
MH  - Gene Expression Regulation, Neoplastic
MH  - Glycogen Synthase Kinase 3 beta/*metabolism
MH  - Humans
MH  - Lung Neoplasms/*genetics/pathology
MH  - Male
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - MicroRNAs/genetics/*metabolism
MH  - Neoplasm Invasiveness
MH  - Signal Transduction
MH  - Up-Regulation/genetics
MH  - beta Catenin/*metabolism
MH  - Mice
OTO - NOTNLM
OT  - Cell proliferation
OT  - ERK/GSK3beta/beta-catenin
OT  - Fibulin 5
OT  - MiR-552-3p
OT  - Migration and invasion
OT  - Non-small cell lung cancer
EDAT- 2021/11/05 06:00
MHDA- 2022/03/03 06:00
CRDT- 2021/11/04 20:20
PHST- 2021/04/12 00:00 [received]
PHST- 2021/10/09 00:00 [revised]
PHST- 2021/10/22 00:00 [accepted]
PHST- 2021/11/05 06:00 [pubmed]
PHST- 2022/03/03 06:00 [medline]
PHST- 2021/11/04 20:20 [entrez]
AID - S0040-8166(21)00188-9 [pii]
AID - 10.1016/j.tice.2021.101672 [doi]
PST - ppublish
SO  - Tissue Cell. 2021 Dec;73:101672. doi: 10.1016/j.tice.2021.101672. Epub 2021 Oct 
      29.