PMID- 34736539
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211108
IS  - 1226-4601 (Print)
IS  - 2055-7124 (Electronic)
IS  - 1226-4601 (Linking)
VI  - 25
IP  - 1
DP  - 2021 Nov 4
TI  - Hepatic and renal cellular cytotoxic effects of heparin-coated superparamagnetic 
      Iron oxide nanoparticles.
PG  - 36
LID - 10.1186/s40824-021-00241-7 [doi]
LID - 36
AB  - BACKGROUND: Superparamagnetic iron oxide (SPIO) nanoparticles have been widely 
      used in several biomedical engineering in vivo. Although various surface 
      modifications have been made to these non-biodegradable nanoparticles to make 
      them more biocompatible, their toxic potential still remains a major concern. 
      METHOD: In this study, we newly developed unfractionated heparin (UFH)-coated and 
      low molecular weight heparin (LMWH)-coated SPIO nanoparticles through surface 
      modification engineering, which was compared with commercially available 
      dextran-coated SPIO nanoparticles. Their toxicity such as cytotoxicity, single 
      cell gel electrophoresis (SCGE) comet assay, intracellular reactive oxygen 
      species (ROS) content and cellular apoptosis was evaluated to hepatic HepG2 and 
      renal HK-2 cells. RESULTS: When UFH-, LMWH- or dextran-coated SPIO nanoparticles 
      were applied, they did not affect the viability of HepG2 cell. However, HK-2 
      cells were more sensitive to dextran-coated SPIO nanoparticles than others. In 
      genotoxicity assay using SCGE comet, DNA tail moment values in the groups treated 
      with dextran- and LMWH-coated SPIO nanoparticles significantly increased. 
      However, UFH-coated SPIO nanoparticles was only significantly lowing DNA tail 
      moment value. In addition, UFH-coated SPIO nanoparticles had lower cytotoxicity 
      in HepG2 and HK-2 cells compared to dextran-coated SPIO nanoparticles, especially 
      in terms of apoptosis and intracellular ROS production. CONCLUSIONS: 
      Collectively, it is possible that UFH- coated SPIO nanoparticles can be used as 
      alternative negative contrast agents.
CI  - (c) 2021. The Author(s).
FAU - Hwang, Yong Hwa
AU  - Hwang YH
AD  - Department of Bioengineering, College of Engineering, and BK21 PLUS Future 
      Biopharmaceutical Human Resources Training and Research Team, Institute of Nano 
      Science & Technology (INST), Hanyang University, Seoul, 04763, Republic of Korea.
FAU - Kim, Youn-Jung
AU  - Kim YJ
AD  - Department of Marine Science, College of Natural Sciences, Incheon National 
      University, Incheon, 22012, Republic of Korea.
AD  - Research Institute of Basic Sciences, Incheon National University, Incheon, 
      22012, Republic of Korea.
FAU - Lee, Dong Yun
AU  - Lee DY
AUID- ORCID: 0000-0001-7691-0447
AD  - Department of Bioengineering, College of Engineering, and BK21 PLUS Future 
      Biopharmaceutical Human Resources Training and Research Team, Institute of Nano 
      Science & Technology (INST), Hanyang University, Seoul, 04763, Republic of Korea. 
      dongyunlee@hanyang.ac.kr.
AD  - Elixir Pharmatech Inc., Seoul, 04763, Republic of Korea. 
      dongyunlee@hanyang.ac.kr.
LA  - eng
GR  - NRF-2020R1A2C3005834/National Research Foundation of Korea/
GR  - NRF-2017R1A6A1A06015181/National Research Foundation of Korea/
PT  - Journal Article
DEP - 20211104
PL  - United States
TA  - Biomater Res
JT  - Biomaterials research
JID - 101650636
PMC - PMC8567628
OTO - NOTNLM
OT  - Cytotoxicity
OT  - Dextran
OT  - Heparin
OT  - Negative contrast agents
OT  - Superparamagnetic iron oxide (SPIO)
COIS- The authors declare that they have no competing interest.
EDAT- 2021/11/06 06:00
MHDA- 2021/11/06 06:01
PMCR- 2021/11/04
CRDT- 2021/11/05 05:36
PHST- 2021/08/25 00:00 [received]
PHST- 2021/10/25 00:00 [accepted]
PHST- 2021/11/05 05:36 [entrez]
PHST- 2021/11/06 06:00 [pubmed]
PHST- 2021/11/06 06:01 [medline]
PHST- 2021/11/04 00:00 [pmc-release]
AID - 10.1186/s40824-021-00241-7 [pii]
AID - 241 [pii]
AID - 10.1186/s40824-021-00241-7 [doi]
PST - epublish
SO  - Biomater Res. 2021 Nov 4;25(1):36. doi: 10.1186/s40824-021-00241-7.