PMID- 34737359
OWN - NLM
STAT- MEDLINE
DCOM- 20220125
LR  - 20240404
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Nov 4
TI  - Selenium-enriched Bacillus subtilis yb-114246 improved growth and immunity of 
      broiler chickens through modified ileal bacterial composition.
PG  - 21690
LID - 10.1038/s41598-021-00699-4 [doi]
LID - 21690
AB  - Here, a Selenium-enriched Bacillus subtilis (SEBS) strain was generated and 
      supplemented to broiler chickens' diet, and the impact in ileum bacterial 
      microbiome, immunity and body weight were assessed. In a nutshell, five hundred 
      1-old old chicken were randomly divided into five groups: control, inorganic Se, 
      Bacillus subtilis (B. subtilis), SEBS, and antibiotic, and colonization with B. 
      subtilis and SEBS in the gastrointestinal tract (GIT) were measured by 
      fluorescence in situ hybridization (FISH) assay and quantitative real-time 
      polymerase chain reaction (qPCR). In summary, Chicks fed SEBS or B. subtilis had 
      higher body weight than the control chicks or those given inorganic Se. SEBS 
      colonized in distal segments of the ileum improved bacterial diversity, reduced 
      the endogenous pathogen burden and increased the number of Lactobacillus sp. in 
      the ileal mucous membrane. Species of unclassified Lachnospiraceae, uncultured 
      Anaerosporobacter, Peptococcus, Lactobacillus salivarius, and 
      Ruminococcaceae_UCG-014, and unclassified Butyricicoccus in the ileal mucous 
      membrane played a key role in promoting immunity. Inorganic Se supplementation 
      also improved bacterial composition of ileal mucous membranes, but to a less 
      extent. In conclusion, SEBS improved performance and immunity of broiler chickens 
      through colonization and modulation of the ileal mucous membrane microbiome.
CI  - (c) 2021. The Author(s).
FAU - Yang, Jiajun
AU  - Yang J
AD  - School of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of 
      Agriculture and Forestry, Jurong, 212400, Jiangsu, China.
AD  - College of Animal Science and Technology, Chinese Agricultural University, 
      Beijing, 100093, China.
FAU - Wang, Jing
AU  - Wang J
AD  - School of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of 
      Agriculture and Forestry, Jurong, 212400, Jiangsu, China.
AD  - College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, 
      China.
FAU - Huang, Kehe
AU  - Huang K
AD  - College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, 
      China.
FAU - Liu, Qingxin
AU  - Liu Q
AD  - School of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of 
      Agriculture and Forestry, Jurong, 212400, Jiangsu, China.
FAU - GuofangLiu
AU  - GuofangLiu
AD  - School of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of 
      Agriculture and Forestry, Jurong, 212400, Jiangsu, China.
FAU - Xu, Xiaozhou
AU  - Xu X
AD  - School of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of 
      Agriculture and Forestry, Jurong, 212400, Jiangsu, China.
FAU - Zhang, Hao
AU  - Zhang H
AD  - College of Animal Science and Technology, Chinese Agricultural University, 
      Beijing, 100093, China. zhanghao827@163.com.
FAU - Zhu, Mengling
AU  - Zhu M
AD  - School of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of 
      Agriculture and Forestry, Jurong, 212400, Jiangsu, China. 
      zhumengling@jsafc.edu.cn.
LA  - eng
GR  - (2021kj19)/Youth support project of Jiangsu Vocational College of Agriculture and 
      Forestry/
GR  - (202113103058Y)/Innovation training program for College Students/
GR  - (CARS-40)/China Agriculture Research System of MOF and MARA/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211104
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - H6241UJ22B (Selenium)
SB  - IM
MH  - Animal Feed/analysis
MH  - Animal Husbandry/methods
MH  - Animals
MH  - Bacillus subtilis/metabolism/physiology
MH  - Chickens/*growth & development/immunology/*microbiology
MH  - Diet/veterinary
MH  - Dietary Supplements/analysis
MH  - Gastrointestinal Microbiome/drug effects
MH  - Ileum/microbiology
MH  - Probiotics/analysis
MH  - Selenium/metabolism/*pharmacology
PMC - PMC8568892
COIS- The authors declare no competing interests.
EDAT- 2021/11/06 06:00
MHDA- 2022/01/27 06:00
PMCR- 2021/11/04
CRDT- 2021/11/05 06:27
PHST- 2021/04/21 00:00 [received]
PHST- 2021/10/14 00:00 [accepted]
PHST- 2021/11/05 06:27 [entrez]
PHST- 2021/11/06 06:00 [pubmed]
PHST- 2022/01/27 06:00 [medline]
PHST- 2021/11/04 00:00 [pmc-release]
AID - 10.1038/s41598-021-00699-4 [pii]
AID - 699 [pii]
AID - 10.1038/s41598-021-00699-4 [doi]
PST - epublish
SO  - Sci Rep. 2021 Nov 4;11(1):21690. doi: 10.1038/s41598-021-00699-4.