PMID- 34738030
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211106
IS  - 2405-6383 (Electronic)
IS  - 2405-6545 (Print)
IS  - 2405-6383 (Linking)
VI  - 7
IP  - 4
DP  - 2021 Dec
TI  - Calcium overload and reactive oxygen species accumulation induced by selenium 
      deficiency promote autophagy in swine small intestine.
PG  - 997-1008
LID - 10.1016/j.aninu.2021.05.005 [doi]
AB  - Selenium (Se) deficiency can seriously affect the small intestine of swine, and 
      cause diarrhea in swine. However, the specific mechanism of Se deficiency-induced 
      swine diarrhea has rarely been reported. Here, to explore the damage of Se 
      deficiency on the calcium homeostasis and autophagy mechanism of swine, in vivo 
      and in vitro models of swine intestinal Se deficiency were established. 
      Twenty-four pure line castrated male Yorkshire pigs (45 d old, 12.50 +/- 1.32 kg, 
      12 full-sibling pairs) were divided into 2 equal groups and fed Se-deficient diet 
      (0.007 mg Se/kg) as the Se-deficiency group, or fed Se-adequate diet (0.3 mg 
      Se/kg) as the control group for 16 weeks. The intestinal porcine enterocyte cell 
      line (IPEC-J2) was divided into 2 groups, and cultured by Se-deficient medium as 
      the Se-deficient group, or cultured by normal medium as the control group. 
      Morphological observations showed that compared with the control group, 
      intestinal cells in the Se-deficiency group were significantly damaged, and 
      autophagosomes increased. Autophagy staining and cytoplasmic calcium staining 
      results showed that in the Se-deficiency group, autophagy increased and calcium 
      homeostasis was destroyed. According to the reactive oxygen species (ROS) 
      staining results, the percentage of ROS in the Se-deficiency group was higher 
      than that in the control group in the in vitro model. Compared with the control 
      group, the protein and mRNA expressions of autophagy-calcium-related genes 
      including Beclin 1, microtubule-associated proteins 1A (LC3-1), 
      microtubule-associated proteins 1B (LC3-2), autophagy-related protein 5 (ATG5), 
      autophagy-related protein 12 (ATG12), autophagy-related protein 16 (ATG16), 
      mammalian target of rapamycin (mTOR), calmodulin-dependent protein kinase kinase 
      beta (CAMKK-beta), adenosine 5'-monophosphate-activated protein kinase (AMPK), 
      sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA), and calpain in the 
      Se-deficiency group were significantly increased which was consistent in vivo and 
      in vitro (P < 0.05). Altogether, our results indicated that Se deficiency could 
      destroy the calcium homeostasis of the swine small intestine to trigger cell 
      autophagy and oxidative stress, which was helpful to explain the mechanism of Se 
      deficiency-induced diarrhea in swine.
CI  - (c) 2021 Chinese Association of Animal Science and Veterinary Medicine. Publishing 
      services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.
FAU - Zheng, Yingying
AU  - Zheng Y
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Guan, Haoyue
AU  - Guan H
AD  - College of Animal Science and Veterinary Medicine, Southwest Minzu University, 
      Chengdu, Sichuan, China.
FAU - Yang, Jie
AU  - Yang J
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Cai, Jingzeng
AU  - Cai J
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Liu, Qi
AU  - Liu Q
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Zhang, Ziwei
AU  - Zhang Z
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
AD  - Key Laboratory of the Provincial Education, Department of Heilongjiang for Common 
      Animal Disease Prevention and Treatment, College of Veterinary Medicine, 
      Northeast Agricultural University, Harbin, China.
LA  - eng
PT  - Journal Article
DEP - 20210922
PL  - China
TA  - Anim Nutr
JT  - Animal nutrition (Zhongguo xu mu shou yi xue hui)
JID - 101717655
PMC - PMC8536506
OTO - NOTNLM
OT  - Autophagy
OT  - Ca overload
OT  - Intestinal porcine enterocyte cell line
OT  - Se deficiency swine
OT  - Small intestine
EDAT- 2021/11/06 06:00
MHDA- 2021/11/06 06:01
PMCR- 2021/09/22
CRDT- 2021/11/05 06:52
PHST- 2020/12/03 00:00 [received]
PHST- 2021/03/22 00:00 [revised]
PHST- 2021/05/07 00:00 [accepted]
PHST- 2021/11/05 06:52 [entrez]
PHST- 2021/11/06 06:00 [pubmed]
PHST- 2021/11/06 06:01 [medline]
PHST- 2021/09/22 00:00 [pmc-release]
AID - S2405-6545(21)00139-6 [pii]
AID - 10.1016/j.aninu.2021.05.005 [doi]
PST - ppublish
SO  - Anim Nutr. 2021 Dec;7(4):997-1008. doi: 10.1016/j.aninu.2021.05.005. Epub 2021 
      Sep 22.