PMID- 34738720
OWN - NLM
STAT- MEDLINE
DCOM- 20220104
LR  - 20220104
IS  - 1522-7243 (Electronic)
IS  - 1522-7235 (Linking)
VI  - 37
IP  - 1
DP  - 2022 Jan
TI  - Sensitivity improvement for spectrofluorimetric determination of commonly used 
      antifungal drug 'nystatin': application for oral suspension.
PG  - 134-140
LID - 10.1002/bio.4154 [doi]
AB  - In this paper, two simple, rapid and highly sensitive spectrofluorimetric methods 
      were developed and validated for nystatin determination in its pure form and 
      pharmaceutical dosage form (oral suspension). The first method was based on 
      measuring the nystatin native fluorescence after dilution with isopropyl alcohol 
      at 407 nm (excitation 303 nm). The fluoresence intensity was linearly dependant 
      on the nystatin concentration within the specified range 50-500 ng ml(-1) . The 
      second was based on micellar enhancement of nystatin fluorescence using sodium 
      dodecyl sulphate (SDS). In the presence of 2% w/v SDS, an ~1.9-fold enhancement 
      could be achieved in the relative fluorescence intensity of nystatin. The linear 
      range for the second method was 20-100 ng ml(-1) . The limits of quantification 
      and detection were found to be 43.23 ng ml(-1) and 14.27 ng ml(-1) (Method I), 
      6.08 ng ml(-1) and 2.0 ng ml(-1) (Method II). According to percentage recoveries 
      and relative standard deviations (RSDs) obtained, the proposed methods were 
      precise (RSDs were less than 2%), reproducible, and accurate and could be 
      successfully applied for quantitative estimation of nystatin in its dosage form. 
      The statistical results of this method were compared with that of the reported 
      method and showed excellent agreement with respect to accuracy and precision.
CI  - (c) 2021 John Wiley & Sons Ltd.
FAU - Attia, Tamer Z
AU  - Attia TZ
AUID- ORCID: 0000-0002-7241-2130
AD  - Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, 
      Egypt.
FAU - Saied, Huda H
AU  - Saied HH
AD  - Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, 
      Egypt.
FAU - Nour El-Deen, Deena A M
AU  - Nour El-Deen DAM
AD  - Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, 
      Egypt.
FAU - Alahmadi, Yaser M
AU  - Alahmadi YM
AD  - Department of Clinical and Hospital Pharmacy, College of Pharmacy, Taibah 
      University, Medina, Kingdom of Saudi Arabia.
FAU - Omar, Mahmoud A
AU  - Omar MA
AUID- ORCID: 0000-0001-5245-8767
AD  - Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, 
      Egypt.
AD  - Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy, 
      Taibah University, Medinah, Saudi Arabia.
LA  - eng
PT  - Journal Article
DEP - 20211120
PL  - England
TA  - Luminescence
JT  - Luminescence : the journal of biological and chemical luminescence
JID - 100889025
RN  - 0 (Antifungal Agents)
RN  - 0 (Micelles)
RN  - 1400-61-9 (Nystatin)
RN  - 368GB5141J (Sodium Dodecyl Sulfate)
SB  - IM
MH  - *Antifungal Agents
MH  - Micelles
MH  - *Nystatin
MH  - Sodium Dodecyl Sulfate
MH  - Spectrometry, Fluorescence
OTO - NOTNLM
OT  - antifungal
OT  - micellar enhancement
OT  - native fluorescence
OT  - nystatin
EDAT- 2021/11/06 06:00
MHDA- 2022/01/05 06:00
CRDT- 2021/11/05 08:48
PHST- 2021/09/16 00:00 [revised]
PHST- 2021/07/24 00:00 [received]
PHST- 2021/10/27 00:00 [accepted]
PHST- 2021/11/06 06:00 [pubmed]
PHST- 2022/01/05 06:00 [medline]
PHST- 2021/11/05 08:48 [entrez]
AID - 10.1002/bio.4154 [doi]
PST - ppublish
SO  - Luminescence. 2022 Jan;37(1):134-140. doi: 10.1002/bio.4154. Epub 2021 Nov 20.