PMID- 34739656
OWN - NLM
STAT- MEDLINE
DCOM- 20220520
LR  - 20220531
IS  - 1567-2387 (Electronic)
IS  - 1567-2379 (Linking)
VI  - 53
IP  - 2
DP  - 2022 Apr
TI  - CircDLGAP4 overexpression relieves oxygen-glucose deprivation-induced neuronal 
      injury by elevating NEGR1 through sponging miR-503-3p.
PG  - 321-332
LID - 10.1007/s10735-021-10036-8 [doi]
AB  - Circular RNAs (circRNAs) have been reported to play vital regulatory roles in 
      human diseases. However, the functions of circRNAs in ischemic stroke (IS) are 
      limited. In this study, we aimed to explore the functions and mechanisms of 
      circRNA DLG associated protein 4 (circDLGAP4) in IS development. Oxygen-glucose 
      deprivation (OGD)-treated HCN-2 cells were used to mimic IS environment in vitro. 
      Quantitative real-time polymerase chain reaction (qRT-PCR) assay was used to 
      detect the levels of circDLGAP4, microRNA-503-3p (miR-503-3p) and neuronal growth 
      regulator 1 (NEGR1) mRNA. RNase R assay was conducted to analyze the stability of 
      circDLGAP4. Cell Counting Kit-8 (CCK-8) assay and flow cytometry analysis were 
      adopted for cell viability and death, respectively. Western blot assay was 
      performed for protein levels. Enzyme-linked immunosorbent assay (ELISA) kits were 
      used to examine the concentrations of inflammatory cytokines. Dual-luciferase 
      reporter assay, RNA immunoprecipitation (RIP) assay and RNA pull-down assay were 
      employed to analyze the relationships among circDLGAP4, miR-503-3p and NEGR1. 
      CircDLGAP4 level was declined in HCN-2 cells after OGD treatment. CircDLGAP4 
      overexpression promoted cell viability and suppressed cell death and inflammatory 
      cytokine concentrations in OGD-treated HCN-2 cells. CircDLGAP4 acted as the 
      sponge for miR-503-3p and the impacts of circDLGAP4 overexpression on cell 
      viability, death and inflammation in OGD-treated HCN-2 cells were reversed by 
      miR-503-3p elevation. Furthermore, NEGR1 was the target gene of miR-503-3p. 
      MiR-503-3p inhibition ameliorated OGD-induced HCN-2 cell impairments, but NEGR1 
      knockdown abolished the effects. CircDLGAP4 alleviated OGD-induced HCN-2 cell 
      damage by regulating miR-503-3p/NEGR1 axis.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer Nature B.V.
FAU - Qiu, Lingling
AU  - Qiu L
AD  - Department of Neurology, Taizhou Municipal Hospital, No.381 Zhongshan East Road, 
      Jiaojiang District, Taizhou, 318000, Zhejiang Province, China.
FAU - He, Jinfeng
AU  - He J
AD  - Department of Neurology, Taizhou Municipal Hospital, No.381 Zhongshan East Road, 
      Jiaojiang District, Taizhou, 318000, Zhejiang Province, China.
FAU - Chen, Hui
AU  - Chen H
AD  - Department of Neurology, Taizhou Municipal Hospital, No.381 Zhongshan East Road, 
      Jiaojiang District, Taizhou, 318000, Zhejiang Province, China.
FAU - Xu, Xiaohui
AU  - Xu X
AD  - Department of Neurology, Taizhou Municipal Hospital, No.381 Zhongshan East Road, 
      Jiaojiang District, Taizhou, 318000, Zhejiang Province, China.
FAU - Tao, Yongjun
AU  - Tao Y
AD  - Department of Neurology, Taizhou Municipal Hospital, No.381 Zhongshan East Road, 
      Jiaojiang District, Taizhou, 318000, Zhejiang Province, China. 
      taoyongjun19910121@163.com.
LA  - eng
GR  - No.1701KY39/Taizhou Bureau of Science and Technology/
GR  - No. 2019KY783/Health Science and Technology Project of Zhejiang Province/
PT  - Journal Article
DEP - 20211105
PL  - Netherlands
TA  - J Mol Histol
JT  - Journal of molecular histology
JID - 101193653
RN  - 0 (Cell Adhesion Molecules, Neuronal)
RN  - 0 (DLGAP4 protein, human)
RN  - 0 (GPI-Linked Proteins)
RN  - 0 (MIRN503 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (NEGR1 protein, human)
RN  - 0 (RNA, Circular)
RN  - 0 (SAP90-PSD95 Associated Proteins)
RN  - IY9XDZ35W2 (Glucose)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - *Cell Adhesion Molecules, Neuronal/metabolism
MH  - *GPI-Linked Proteins/metabolism
MH  - Glucose/metabolism
MH  - Humans
MH  - *MicroRNAs/genetics/metabolism
MH  - Neurons/metabolism
MH  - Oxygen/metabolism
MH  - *RNA, Circular/genetics
MH  - SAP90-PSD95 Associated Proteins
OTO - NOTNLM
OT  - IS
OT  - NEGR1
OT  - OGD
OT  - circDLGAP4
OT  - miR-503-3p
EDAT- 2021/11/06 06:00
MHDA- 2022/05/21 06:00
CRDT- 2021/11/05 17:26
PHST- 2021/06/02 00:00 [received]
PHST- 2021/10/28 00:00 [accepted]
PHST- 2021/11/06 06:00 [pubmed]
PHST- 2022/05/21 06:00 [medline]
PHST- 2021/11/05 17:26 [entrez]
AID - 10.1007/s10735-021-10036-8 [pii]
AID - 10.1007/s10735-021-10036-8 [doi]
PST - ppublish
SO  - J Mol Histol. 2022 Apr;53(2):321-332. doi: 10.1007/s10735-021-10036-8. Epub 2021 
      Nov 5.