PMID- 3474028
OWN - NLM
STAT- MEDLINE
DCOM- 19870727
LR  - 20190609
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 900
IP  - 1
DP  - 1987 Jun 12
TI  - Phospholipid asymmetry during erythropoiesis. A study on Friend erythroleukemic 
      cells and mouse reticulocytes.
PG  - 103-15
AB  - The distribution of phospholipids over the outer and inner layers of the plasma 
      membranes of differentiated Friend erythroleukemic cells (Friend cells) and mouse 
      reticulocytes has been determined. Phosphatidylcholine, phosphatidylethanolamine 
      and phosphatidylinositol were found to be distributed symmetrically over both 
      layers, sphingomyelin was found to be enriched in the outer layer (80-85%) and 
      phosphatidylserine appeared to be present mainly in the inner layer (80-90%) of 
      the plasma membranes of differentiated Friend cells. The outer layer of 
      reticulocyte membranes contains 50-60% of the phosphatidylcholine, 20% of the 
      phosphatidylethanolamine, 82-85% of the sphingomyelin and 40-42% of the 
      phosphatidylinositol. All of the phosphatidylserine is present in the inner 
      layer. The results show, that the asymmetric distribution of phospholipids, 
      typical for erythrocyte membranes, is partially apparent already at an early 
      stage of erythropoiesis, the proerythroblast, while the final organization of 
      phospholipid distribution takes place at some stage during enucleation of the 
      enormoblast and release of the reticulocyte into the blood stream.
FAU - Van der Schaft, P H
AU  - Van der Schaft PH
FAU - Roelofsen, B
AU  - Roelofsen B
FAU - Op den Kamp, J A
AU  - Op den Kamp JA
FAU - Van Deenen, L L
AU  - Van Deenen LL
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Lipid Bilayers)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Phospholipids)
RN  - 38183-12-9 (Fluorescamine)
RN  - EC 3.1.- (Phospholipases)
SB  - IM
MH  - Animals
MH  - Erythrocyte Membrane/*metabolism
MH  - *Erythropoiesis
MH  - Fluorescamine
MH  - Friend murine leukemia virus
MH  - Leukemia, Erythroblastic, Acute/*metabolism
MH  - Lipid Bilayers/*blood
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred DBA
MH  - Phosphatidylcholines/blood
MH  - Phospholipases/metabolism
MH  - Phospholipids/*blood
MH  - Reticulocytes/*metabolism
EDAT- 1987/06/12 00:00
MHDA- 1987/06/12 00:01
CRDT- 1987/06/12 00:00
PHST- 1987/06/12 00:00 [pubmed]
PHST- 1987/06/12 00:01 [medline]
PHST- 1987/06/12 00:00 [entrez]
AID - 0005-2736(87)90282-3 [pii]
AID - 10.1016/0005-2736(87)90282-3 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1987 Jun 12;900(1):103-15. doi: 
      10.1016/0005-2736(87)90282-3.