PMID- 34740292
OWN - NLM
STAT- MEDLINE
DCOM- 20211109
LR  - 20211109
IS  - 1873-6424 (Electronic)
IS  - 0269-7491 (Linking)
VI  - 291
DP  - 2021 Dec 15
TI  - Inhibition of hydroxylated polychlorinated biphenyls (OH-PCBs) on 
      sulfotransferases (SULTs).
PG  - 118214
LID - S0269-7491(21)01796-6 [pii]
LID - 10.1016/j.envpol.2021.118214 [doi]
AB  - Polychlorinated biphenyls (PCBs) have been demonstrated as a kind of the 
      persistent organic pollutants (POPs) that could exert complicated influences 
      towards metabolism in human bodies. Since hydroxylated polychlorinated biphenyls 
      (OH-PCBs) are important metabolites of PCBs, our study focuses on investigating 
      the potential inhibitory capability of OH-PCBs on four human sulfotransferase 
      (SULT) isoforms. P-nitrophenol (PNP) was utilized as nonselective probe substrate 
      for this study, and recombinant SULT isoforms were utilized as the enzyme 
      resources. Ultra-performance liquid chromatography (UPLC)-UV detecting system was 
      used to analyze PNP and its metabolite PNP-sulfate. As result, 100 muM of most 
      tested OH-PCBs significantly inhibited the activity of four SULT isoforms. 
      Concentration-dependent inhibition of OH-PCBs towards SULTs was found, and half 
      inhibition concentration values (IC(50)) of some inhibition processes were 
      determined. Inhibition kinetics (inhibition kinetic type and parameters) were 
      determined using 4'-OH-PCB106 as the representative OH-PCB, SULT1B1 and SULT1E1 
      as representative SULT isoforms. The inhibition kinetic parameters (K(i)) were 
      1.73 muM and 1.81 muM for the inhibition of 4'-OH-PCB106 towards SULT1B1 and 
      SULT1E1, respectively. In silico docking simulation was utilized to analyze the 
      inhibition capability of 2'-OH-PCB5, 4'-OH-PCB9, 2'-OH-PCB12 towards SULT1A3.All 
      these results obtained in this study are helpful for further understanding the 
      toxicity of PCBs.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Jia, Ruo-Yong
AU  - Jia RY
AD  - Department of Toxicology and Sanitary Chemistry, School of Public Health, Tianjin 
      Medical University, Tianjin, 300070, China.
FAU - Zhang, Zhi-Peng
AU  - Zhang ZP
AD  - Department of Surgery, Peking University Third Hospital, Beijing, China.
FAU - Qin, Guo-Qiang
AU  - Qin GQ
AD  - Department of Toxicology and Sanitary Chemistry, School of Public Health, Tianjin 
      Medical University, Tianjin, 300070, China.
FAU - Zhang, Wei
AU  - Zhang W
AD  - Department of Toxicology and Sanitary Chemistry, School of Public Health, Tianjin 
      Medical University, Tianjin, 300070, China.
FAU - Yang, Kun
AU  - Yang K
AD  - Department of Toxicology and Sanitary Chemistry, School of Public Health, Tianjin 
      Medical University, Tianjin, 300070, China.
FAU - Liu, Yong-Zhe
AU  - Liu YZ
AD  - Department of Toxicology and Sanitary Chemistry, School of Public Health, Tianjin 
      Medical University, Tianjin, 300070, China.
FAU - Jiang, Changtao
AU  - Jiang C
AD  - Department of Physiology and Pathophysiology, School of Basic Medical Sciences, 
      Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Peking 
      University, Beijing, China.
FAU - Fang, Zhong-Ze
AU  - Fang ZZ
AD  - Department of Toxicology and Sanitary Chemistry, School of Public Health, Tianjin 
      Medical University, Tianjin, 300070, China; Tianjin Key Laboratory of 
      Environment, Nutrition and Public Health, Tianjin, China; National Demonstration 
      Center for Experimental Preventive Medicine Education, Tianjin Medical 
      University, Tianjin, 300070, China; Tianjin Center for International 
      Collaborative Research in Environment, Nutrition and Public Health, Tianjin, 
      China. Electronic address: fangzhongze@tmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20210923
PL  - England
TA  - Environ Pollut
JT  - Environmental pollution (Barking, Essex : 1987)
JID - 8804476
RN  - 0 (Sulfates)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
RN  - EC 2.8.2.- (Sulfotransferases)
SB  - IM
MH  - Chromatography, Liquid
MH  - Humans
MH  - Hydroxylation
MH  - *Polychlorinated Biphenyls/toxicity
MH  - Sulfates
MH  - Sulfotransferases/metabolism
OTO - NOTNLM
OT  - Human sulfotransferases (SULTs)
OT  - Hydroxylated polychlorinated biphenyls (OH-PCBs)
OT  - In vitro-in vivo extrapolation (IVIVE)
OT  - Inhibition
EDAT- 2021/11/07 06:00
MHDA- 2021/11/10 06:00
CRDT- 2021/11/06 01:01
PHST- 2021/04/01 00:00 [received]
PHST- 2021/09/17 00:00 [revised]
PHST- 2021/09/19 00:00 [accepted]
PHST- 2021/11/06 01:01 [entrez]
PHST- 2021/11/07 06:00 [pubmed]
PHST- 2021/11/10 06:00 [medline]
AID - S0269-7491(21)01796-6 [pii]
AID - 10.1016/j.envpol.2021.118214 [doi]
PST - ppublish
SO  - Environ Pollut. 2021 Dec 15;291:118214. doi: 10.1016/j.envpol.2021.118214. Epub 
      2021 Sep 23.