PMID- 34740808
OWN - NLM
STAT- MEDLINE
DCOM- 20211207
LR  - 20211214
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 161
IP  - Pt B
DP  - 2021 Dec
TI  - Effects of type 2 diabetes and metformin on salivary microbiota in patients with 
      chronic periodontitis.
PG  - 105277
LID - S0882-4010(21)00551-9 [pii]
LID - 10.1016/j.micpath.2021.105277 [doi]
AB  - Diabetes is closely associated with periodontitis, however, the effects of type 2 
      diabetes and metformin treatment on the salivary microbiota in chronic 
      periodontitis patients are still insufficiently studied. Saliva was collected 
      from ten patients with moderate to severe chronic periodontitis (CP group) and 20 
      patients with type 2 diabetes mellitus (T2DM) and moderate to severe chronic 
      periodontitis (ten patients were newly diagnosed with diabetes without drug 
      treatment (DM group), and ten patients were treated with metformin (CP-DM-MET 
      group)). Total DNA was extracted. DNA amplicons of the V3-V4 hypervariable 
      regions of the 16S rRNA gene were generated and subjected to high-throughput 
      sequencing. There was no significant difference in the alpha diversity of the 
      salivary microbiota (Observed_Species, Shannon, Simpson, ACE, Chao1 index) among 
      the three groups. The dominant phyla with relative abundances greater than 1% 
      were Firmicutes, Proteobacteria, Bacteroidota, Actinobacteriota, Fusobacteriota, 
      and Spirochaetota, and no significant difference was found among the three 
      groups. Compared with the CP group, the relative abundance of twelve genera was 
      found changed in CP-DM group, for example, Aggregatibacter, 
      Unclassified_f_Neisseriaceae, Parvimonas, Erysipelotrichace_UCG-006, Atopobium, 
      and Endomicrobium et al. Metformin treatment could partly restore the abundance 
      of several genera in CP-DM, such as Acholeplasma and Comamonas. Compared with the 
      CP group, genus Lactobacillus, Parvimonas, 
      Norank_f_norank_o_Absconditabacteriales_SR1, and Acholeplasma changed 
      significantly in CP-DM-MET group. Plaque index (PLI) was positively correlated 
      with Prevotella and Lactobacillus but negatively correlated with Haemophilus, 
      Lautropia, Unclassified_f_Pasteurellaceae, and TM7x. In conclusion, there was a 
      significant difference in the salivary microbiota of patients with chronic 
      periodontitis complicated by T2DM. Treatment with metformin partially alleviated 
      the alteration in salivary microbiota caused by T2DM.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Gu, Min
AU  - Gu M
AD  - Department of Stomatology, The Third Affiliated Hospital of Soochow 
      University/The First People's Hospital of Changzhou, Changzhou, Jiangsu Province, 
      213003, China.
FAU - Wang, Peipei
AU  - Wang P
AD  - Department of Stomatology, The Third Affiliated Hospital of Soochow 
      University/The First People's Hospital of Changzhou, Changzhou, Jiangsu Province, 
      213003, China.
FAU - Xiang, Shoukui
AU  - Xiang S
AD  - Department of Endocrinology, The Third Affiliated Hospital of Soochow 
      University/The First People's Hospital of Changzhou, Changzhou, Jiangsu Province, 
      213003, China.
FAU - Xu, Dan
AU  - Xu D
AD  - Department of Stomatology, The Third Affiliated Hospital of Soochow 
      University/The First People's Hospital of Changzhou, Changzhou, Jiangsu Province, 
      213003, China.
FAU - Jin, Chun
AU  - Jin C
AD  - Department of Stomatology, The Third Affiliated Hospital of Soochow 
      University/The First People's Hospital of Changzhou, Changzhou, Jiangsu Province, 
      213003, China.
FAU - Jiang, Zhenwei
AU  - Jiang Z
AD  - Department of Pharmacy, The Third Affiliated Hospital of Soochow University/The 
      First People's Hospital of Changzhou, Changzhou, Jiangsu Province, 213003, China.
FAU - Hu, Nan
AU  - Hu N
AD  - Department of Pharmacy, The Third Affiliated Hospital of Soochow University/The 
      First People's Hospital of Changzhou, Changzhou, Jiangsu Province, 213003, China. 
      Electronic address: hn_324@163.com.
LA  - eng
PT  - Journal Article
DEP - 20211103
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 9100L32L2N (Metformin)
SB  - IM
MH  - *Chronic Periodontitis
MH  - *Diabetes Mellitus, Type 2/complications/drug therapy
MH  - Humans
MH  - *Metformin/therapeutic use
MH  - *Microbiota
MH  - RNA, Ribosomal, 16S/genetics
MH  - Saliva
OTO - NOTNLM
OT  - Chronic periodontitis
OT  - Metformin
OT  - Salivary microbiota
OT  - Type 2 diabetes
EDAT- 2021/11/07 06:00
MHDA- 2021/12/15 06:00
CRDT- 2021/11/06 05:35
PHST- 2021/07/05 00:00 [received]
PHST- 2021/10/11 00:00 [revised]
PHST- 2021/11/01 00:00 [accepted]
PHST- 2021/11/07 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/11/06 05:35 [entrez]
AID - S0882-4010(21)00551-9 [pii]
AID - 10.1016/j.micpath.2021.105277 [doi]
PST - ppublish
SO  - Microb Pathog. 2021 Dec;161(Pt B):105277. doi: 10.1016/j.micpath.2021.105277. 
      Epub 2021 Nov 3.