PMID- 34743190
OWN - NLM
STAT- MEDLINE
DCOM- 20220311
LR  - 20220418
IS  - 1476-5551 (Electronic)
IS  - 0887-6924 (Linking)
VI  - 36
IP  - 3
DP  - 2022 Mar
TI  - The GPI-anchored protein CD109 protects hematopoietic progenitor cells from 
      undergoing erythroid differentiation induced by TGF-beta.
PG  - 847-855
LID - 10.1038/s41375-021-01463-3 [doi]
AB  - Although a glycosylphosphatidylinositol-anchored protein (GPI-AP) CD109 serves as 
      a TGF-beta co-receptor and inhibits TGF-beta signaling in keratinocytes, the role of 
      CD109 on hematopoietic stem progenitor cells (HSPCs) remains unknown. We studied 
      the effect of CD109 knockout (KO) or knockdown (KD) on TF-1, a myeloid leukemia 
      cell line that expresses CD109, and primary human HSPCs. CD109-KO or KD TF-1 
      cells underwent erythroid differentiation in the presence of TGF-beta. CD109 was 
      more abundantly expressed in hematopoietic stem cells (HSCs) than in multipotent 
      progenitors and HSPCs of human bone marrow (BM) and cord blood but was not 
      detected in mouse HSCs. Erythroid differentiation was induced by TGF-beta to a 
      greater extent in CD109-KD cord blood or iPS cell-derived 
      megakaryocyte-erythrocyte progenitor cells (MEPs) than in wild-type MEPs. When we 
      analyzed the phenotype of peripheral blood MEPs of patients with paroxysmal 
      nocturnal hemoglobinuria who had both GPI(+) and GPI(-) CD34(+) cells, the CD36 
      expression was more evident in CD109(-) MEPs than CD109(+) MEPs. In summary, 
      CD109 suppresses TGF-beta signaling in HSPCs, and the lack of CD109 may increase the 
      sensitivity of PIGA-mutated HSPCs to TGF-beta, thus leading to the preferential 
      commitment of erythroid progenitor cells to mature red blood cells in 
      immune-mediated BM failure.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer Nature Limited.
FAU - Tanabe, Mikoto
AU  - Tanabe M
AD  - Department of Hematology, Faculty of Medicine, Institute of Medical 
      Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
FAU - Hosokawa, Kohei
AU  - Hosokawa K
AUID- ORCID: 0000-0002-3423-1523
AD  - Department of Hematology, Faculty of Medicine, Institute of Medical 
      Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
FAU - Nguyen, Mai Anh Thi
AU  - Nguyen MAT
AD  - Department of Hematology, Faculty of Medicine, Institute of Medical 
      Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
AD  - Pediatric Department, University of Medicine and Pharmacy at HCMC, Ho Chi Minh 
      City, Vietnam.
FAU - Nakagawa, Noriharu
AU  - Nakagawa N
AD  - Department of Hematology, Faculty of Medicine, Institute of Medical 
      Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
FAU - Maruyama, Kana
AU  - Maruyama K
AD  - Department of Hematology, Faculty of Medicine, Institute of Medical 
      Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
FAU - Tsuji, Noriaki
AU  - Tsuji N
AD  - Department of Hematology, Faculty of Medicine, Institute of Medical 
      Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
FAU - Urushihara, Ryota
AU  - Urushihara R
AD  - Department of Hematology, Faculty of Medicine, Institute of Medical 
      Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
FAU - Espinoza, Luis
AU  - Espinoza L
AD  - Department of Clinical Laboratory Sciences, Kanazawa University Graduate School, 
      Kanazawa, Japan.
FAU - Elbadry, Mahmoud I
AU  - Elbadry MI
AUID- ORCID: 0000-0001-5487-9493
AD  - Department of Internal Medicine, Division of Hematology, Faculty of Medicine, 
      Sohag University, Sohag, Egypt.
FAU - Mohiuddin, Md
AU  - Mohiuddin M
AUID- ORCID: 0000-0002-7313-8655
AD  - Department of Respiratory Medicine, Kanazawa University, Kanazawa, Japan.
FAU - Katagiri, Takamasa
AU  - Katagiri T
AD  - Department of Clinical Laboratory Sciences, Kanazawa University Graduate School, 
      Kanazawa, Japan.
FAU - Ono, Masanori
AU  - Ono M
AD  - Department of Obstetrics and Gynecology, Graduate School of Medical Sciences, 
      Kanazawa University, Kanazawa, Japan.
FAU - Fujiwara, Hiroshi
AU  - Fujiwara H
AD  - Department of Obstetrics and Gynecology, Graduate School of Medical Sciences, 
      Kanazawa University, Kanazawa, Japan.
FAU - Chonabayashi, Kazuhisa
AU  - Chonabayashi K
AD  - Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan.
AD  - Department of Hematology and Oncology, Graduate School of Medicine, Kyoto 
      University, Kyoto, Japan.
FAU - Yoshida, Yoshinori
AU  - Yoshida Y
AD  - Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan.
FAU - Yamazaki, Hirohito
AU  - Yamazaki H
AUID- ORCID: 0000-0003-3018-2098
AD  - Division of Transfusion Medicine, Kanazawa University Hospital, Kanazawa, Japan.
FAU - Hirao, Atsushi
AU  - Hirao A
AD  - Division of Molecular Genetics, Cancer and Stem Cell Research Program, Cancer 
      Research Institute, Kanazawa University, Kanazawa, Japan.
AD  - WPI Nano Life Science Institute, Kanazawa University, Kanazawa, Japan.
FAU - Nakao, Shinji
AU  - Nakao S
AUID- ORCID: 0000-0002-9674-624X
AD  - Department of Hematology, Faculty of Medicine, Institute of Medical 
      Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan. 
      snakao8205@staff.kanazawa-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211106
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (Antigens, CD)
RN  - 0 (CD109 protein, human)
RN  - 0 (GPI-Linked Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Antigens, CD/*metabolism
MH  - Cell Differentiation
MH  - Cell Line
MH  - Cells, Cultured
MH  - Erythroid Cells/*cytology/metabolism
MH  - Erythropoiesis
MH  - GPI-Linked Proteins/metabolism
MH  - Hematopoiesis
MH  - Hematopoietic Stem Cells/*cytology/metabolism
MH  - Humans
MH  - Neoplasm Proteins/*metabolism
MH  - Transforming Growth Factor beta/*metabolism
EDAT- 2021/11/08 06:00
MHDA- 2022/03/12 06:00
CRDT- 2021/11/07 20:45
PHST- 2021/03/15 00:00 [received]
PHST- 2021/10/21 00:00 [accepted]
PHST- 2021/10/09 00:00 [revised]
PHST- 2021/11/08 06:00 [pubmed]
PHST- 2022/03/12 06:00 [medline]
PHST- 2021/11/07 20:45 [entrez]
AID - 10.1038/s41375-021-01463-3 [pii]
AID - 10.1038/s41375-021-01463-3 [doi]
PST - ppublish
SO  - Leukemia. 2022 Mar;36(3):847-855. doi: 10.1038/s41375-021-01463-3. Epub 2021 Nov 
      6.