PMID- 34743285
OWN - NLM
STAT- MEDLINE
DCOM- 20220322
LR  - 20220322
IS  - 1874-9356 (Electronic)
IS  - 0015-5632 (Linking)
VI  - 67
IP  - 2
DP  - 2022 Apr
TI  - Biotechnology approach using watermelon rind for optimization of alpha-amylase enzyme 
      production from Trichoderma virens using response surface methodology under 
      solid-state fermentation.
PG  - 253-264
LID - 10.1007/s12223-021-00929-2 [doi]
AB  - Production of amylases by fungi under solid-state fermentation is considered the 
      best methodology for commercial scaling that addresses the ever-escalating needs 
      of the worldwide enzyme market. Here response surface methodology (RSM) was used 
      for the optimization of process variables for alpha-amylase enzyme production from 
      Trichoderma virens using watermelon rinds (WMR) under solid-state fermentation 
      (SSF). The statistical model included four variables, each detected at two 
      levels, followed by model development with partial purification and 
      characterization of alpha-amylase. The partially purified alpha-amylase was characterized 
      with regard to optimum pH, temperature, kinetic constant, and substrate 
      specificity. The results indicated that both pH and moisture content had a 
      significant effect (P < 0.05) on alpha-amylase production (880 U/g) under optimized 
      process conditions at a 3-day incubation time, moisture content of 50%, 30  degrees C, 
      and pH 6.98. Statistical optimization using RSM showed R(2) values of 0.9934, 
      demonstrating the validity of the model. Five alpha-amylases were separated by using 
      DEAE-Sepharose and characterized with a wide range of optimized pH values (pH 
      4.5-9.0), temperature optima (40-60  degrees C), low Km values (2.27-3.3 mg/mL), and high 
      substrate specificity toward large substrates. In conclusion, this study presents 
      an efficient and green approach for utilization of agro-waste for production of 
      the valuable alpha-amylase enzyme using RSM under SSF. RSM was particularly 
      beneficial for the optimization and analysis of the effective process parameters.
CI  - (c) 2021. Institute of Microbiology, Academy of Sciences of the Czech Republic, 
      v.v.i.
FAU - Abdel-Mageed, Heidi M
AU  - Abdel-Mageed HM
AUID- ORCID: 0000-0002-2944-1518
AD  - Molecular Biology Department, Genetic Engineering and Biotechnology Division, 
      National Research Centre, El Behoth St, Dokki, Cairo, Egypt.
FAU - Barakat, Amal Z
AU  - Barakat AZ
AD  - Molecular Biology Department, Genetic Engineering and Biotechnology Division, 
      National Research Centre, El Behoth St, Dokki, Cairo, Egypt.
FAU - Bassuiny, Roqaya I
AU  - Bassuiny RI
AD  - Molecular Biology Department, Genetic Engineering and Biotechnology Division, 
      National Research Centre, El Behoth St, Dokki, Cairo, Egypt.
FAU - Elsayed, Alshaimaa M
AU  - Elsayed AM
AD  - Molecular Biology Department, Genetic Engineering and Biotechnology Division, 
      National Research Centre, El Behoth St, Dokki, Cairo, Egypt.
FAU - Salah, Hala A
AU  - Salah HA
AD  - Molecular Biology Department, Genetic Engineering and Biotechnology Division, 
      National Research Centre, El Behoth St, Dokki, Cairo, Egypt.
FAU - Abdel-Aty, Azza M
AU  - Abdel-Aty AM
AD  - Molecular Biology Department, Genetic Engineering and Biotechnology Division, 
      National Research Centre, El Behoth St, Dokki, Cairo, Egypt.
FAU - Mohamed, Saleh A
AU  - Mohamed SA
AD  - Molecular Biology Department, Genetic Engineering and Biotechnology Division, 
      National Research Centre, El Behoth St, Dokki, Cairo, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20211106
PL  - United States
TA  - Folia Microbiol (Praha)
JT  - Folia microbiologica
JID - 0376757
RN  - EC 3.2.1.- (Amylases)
RN  - EC 3.2.1.1 (alpha-Amylases)
SB  - IM
MH  - Amylases
MH  - *Citrullus/metabolism
MH  - Fermentation
MH  - Hydrogen-Ion Concentration
MH  - *Hypocrea/metabolism
MH  - Industrial Microbiology/methods
MH  - Temperature
MH  - alpha-Amylases/chemistry/metabolism
EDAT- 2021/11/08 06:00
MHDA- 2022/03/23 06:00
CRDT- 2021/11/07 20:48
PHST- 2021/06/10 00:00 [received]
PHST- 2021/10/28 00:00 [accepted]
PHST- 2021/11/08 06:00 [pubmed]
PHST- 2022/03/23 06:00 [medline]
PHST- 2021/11/07 20:48 [entrez]
AID - 10.1007/s12223-021-00929-2 [pii]
AID - 10.1007/s12223-021-00929-2 [doi]
PST - ppublish
SO  - Folia Microbiol (Praha). 2022 Apr;67(2):253-264. doi: 10.1007/s12223-021-00929-2. 
      Epub 2021 Nov 6.