PMID- 34743799
OWN - NLM
STAT- MEDLINE
DCOM- 20220125
LR  - 20220125
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 291
IP  - Pt 1
DP  - 2022 Mar
TI  - A robust biocatalyst based on laccase immobilized superparamagnetic 
      Fe(3)O(4)@SiO(2)-NH(2) nanoparticles and its application for degradation of 
      chlorophenols.
PG  - 132727
LID - S0045-6535(21)03199-4 [pii]
LID - 10.1016/j.chemosphere.2021.132727 [doi]
AB  - The presence of chlorophenols in water and wastewater is considered a serious 
      environmental issue. To eliminate these micropollutants, biodegradation of 
      chlorophenols using enzyme-nanoparticle conjugated biocatalyst, is proposed as an 
      economical and eco-friendly method. Herein, amino-functionalized 
      superparamagnetic Fe(3)O(4)@SiO(2)-NH(2) nanoparticles with core-shell structure 
      were constructed as a promising carrier for immobilization of laccase from 
      Trametes versicolor. Compared with free laccase, Fe(3)O(4)@SiO(2)-NH(2)-Laccase 
      displayed remarkable outcomes in all major areas such as temperature and storage 
      stabilities, and tolerance to organic solvents and metal ions. The biocatalytic 
      performance and reusability of Fe(3)O(4)@SiO(2)-NH(2)-Laccase were evaluated for 
      the degradation of 2,4-dichlorophenol (2,4-DCP) and 2,4,6-trichlorophenol 
      (2,4,6-TCP) in repeated cycles. Even after 10 successive reuses, the degradation 
      rate of 2,4-DCP and 2,4,6-TCP were found to be 54.9% and 68.7%, respectively. The 
      influences of solution pH, initial chlorophenol concentration, and temperature on 
      the degradation rate of these two chlorophenols were evaluated. The degradation 
      intermediate products including dimers, trimers, and tetramers of 2,4-DCP and 
      2,4,6-TCP were identified. Release of chloride ions was observed during the 
      enzymatic degradation of these two chlorophenols. Based on the determination of 
      intermediate products and released chloride ions, the degradation pathway that 
      was involved in dehydrogenation, reactive radical intermediates formation, 
      dechlorination, self-coupling and oligomers/polymers formation was proposed. The 
      toxicity of these two chlorophenols and their intermediates was substantially 
      reduced during the enzymatic degradation. The results of this study might present 
      an alternative clean biotechnology for the remediation of 2,4-DCP and 2,4,6-TCP 
      contaminated water matrices.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Chen, Zhihui
AU  - Chen Z
AD  - School of Water Resources and Environment, China University of Geosciences 
      (Beijing), 29 Xueyuan Road, Haidian District, 100083, Beijing, China.
FAU - Yao, Jun
AU  - Yao J
AD  - School of Water Resources and Environment, China University of Geosciences 
      (Beijing), 29 Xueyuan Road, Haidian District, 100083, Beijing, China. Electronic 
      address: yaojun@cugb.edu.cn.
FAU - Ma, Bo
AU  - Ma B
AD  - School of Water Resources and Environment, China University of Geosciences 
      (Beijing), 29 Xueyuan Road, Haidian District, 100083, Beijing, China.
FAU - Liu, Bang
AU  - Liu B
AD  - School of Water Resources and Environment, China University of Geosciences 
      (Beijing), 29 Xueyuan Road, Haidian District, 100083, Beijing, China.
FAU - Kim, Jonghyok
AU  - Kim J
AD  - School of Water Resources and Environment, China University of Geosciences 
      (Beijing), 29 Xueyuan Road, Haidian District, 100083, Beijing, China.
FAU - Li, Hao
AU  - Li H
AD  - School of Water Resources and Environment, China University of Geosciences 
      (Beijing), 29 Xueyuan Road, Haidian District, 100083, Beijing, China.
FAU - Zhu, Xiaozhe
AU  - Zhu X
AD  - School of Water Resources and Environment, China University of Geosciences 
      (Beijing), 29 Xueyuan Road, Haidian District, 100083, Beijing, China.
FAU - Zhao, Chenchen
AU  - Zhao C
AD  - School of Water Resources and Environment, China University of Geosciences 
      (Beijing), 29 Xueyuan Road, Haidian District, 100083, Beijing, China.
FAU - Amde, Meseret
AU  - Amde M
AD  - School of Water Resources and Environment, China University of Geosciences 
      (Beijing), 29 Xueyuan Road, Haidian District, 100083, Beijing, China; Department 
      of Chemistry, College of Natural and Computational Sciences, Haramaya University, 
      Oromia, Ethiopia.
LA  - eng
PT  - Journal Article
DEP - 20211028
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Chlorophenols)
RN  - 0 (Enzymes, Immobilized)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - EC 1.10.3.2 (Laccase)
SB  - IM
MH  - *Chlorophenols
MH  - Enzymes, Immobilized
MH  - Laccase
MH  - Magnetic Iron Oxide Nanoparticles
MH  - *Nanoparticles
MH  - Silicon Dioxide
MH  - Trametes
OTO - NOTNLM
OT  - Chlorophenols
OT  - Dechlorination
OT  - Degradation mechanism
OT  - Laccase immobilization
OT  - Reusability
OT  - Toxicity
EDAT- 2021/11/09 06:00
MHDA- 2022/01/27 06:00
CRDT- 2021/11/08 05:39
PHST- 2021/07/19 00:00 [received]
PHST- 2021/10/23 00:00 [revised]
PHST- 2021/10/26 00:00 [accepted]
PHST- 2021/11/09 06:00 [pubmed]
PHST- 2022/01/27 06:00 [medline]
PHST- 2021/11/08 05:39 [entrez]
AID - S0045-6535(21)03199-4 [pii]
AID - 10.1016/j.chemosphere.2021.132727 [doi]
PST - ppublish
SO  - Chemosphere. 2022 Mar;291(Pt 1):132727. doi: 10.1016/j.chemosphere.2021.132727. 
      Epub 2021 Oct 28.