PMID- 34749352
OWN - NLM
STAT- MEDLINE
DCOM- 20220331
LR  - 20220531
IS  - 1748-605X (Electronic)
IS  - 1748-6041 (Linking)
VI  - 17
IP  - 1
DP  - 2021 Nov 24
TI  - A functional extracellular matrix biomaterial enriched with VEGFA and bFGF as 
      vehicle of human umbilical cord mesenchymal stem cells in skin wound healing.
LID - 10.1088/1748-605X/ac37b0 [doi]
AB  - The construction of microvascular network is one of the greatest challenges for 
      tissue engineering and cell therapy. Endothelial cells are essential for the 
      construction of network of blood vessels. However, their application meets 
      challenges in clinic due to the limited resource of autologous endothelium. 
      Mesenchymal stem cells can effectively promote the angiogenesis in ischemic 
      tissues for their abilities of endothelial differentiation and paracrine, and 
      abundant sources. Extracellular matrix (ECM) has been widely used as an ideal 
      biomaterial to mimic cellular microenvironment for tissue engineering due to its 
      merits of neutrality, good biocompatibility, degradability, and controllability. 
      In this study, a functional cell derived ECM biomaterial enriched with VEGFA and 
      bFGF by expressing the collagen-binding domain fused factor genes in host cells 
      was prepared. This material could induce endothelial differentiation of human 
      umbilical cord mesenchymal stem cells (hUCMSCs) and promote angiogenesis, which 
      may improve the healing effect of skin injury. Our research not only provides a 
      functional ECM material to inducing angiogenesis by inducing endothelial 
      differentiation of hUCMSCs, but also shed light on the ubiquitous approaches to 
      endow ECM materials different functions by enriching different factors. This 
      study will benefit tissue engineering and regenerative medicine researches.
CI  - (c) 2021 IOP Publishing Ltd.
FAU - Xu, Zhongjuan
AU  - Xu Z
AUID- ORCID: 0000-0002-8333-764X
AD  - School of Nano-Tech and Nano-Bionics, University of Science and Technology of 
      China, Hefei, 230026, Anhui, People's Republic of China.
AD  - CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and 
      Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, Jiangsu, People's 
      Republic of China.
FAU - Cao, Junjun
AU  - Cao J
AD  - Livingchip Lnc., Nanjing 211112, Jiangsu, People's Republic of China.
FAU - Zhao, Zhe
AU  - Zhao Z
AD  - CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and 
      Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, Jiangsu, People's 
      Republic of China.
FAU - Qiao, Yong
AU  - Qiao Y
AD  - Livingchip Lnc., Nanjing 211112, Jiangsu, People's Republic of China.
FAU - Liu, Xingzhi
AU  - Liu X
AUID- ORCID: 0000-0001-8134-4267
AD  - School of Nano-Tech and Nano-Bionics, University of Science and Technology of 
      China, Hefei, 230026, Anhui, People's Republic of China.
AD  - CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and 
      Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, Jiangsu, People's 
      Republic of China.
FAU - Zhong, Junjie
AU  - Zhong J
AD  - Department of Neurosurgery, Fudan University Huashan Hospital, National Key 
      Laboratory of Medical Neurobiology, Institutes of Brain Science, Shanghai Medical 
      College, Fudan University, Shanghai 200041, People's Republic of China.
FAU - Wang, Bin
AU  - Wang B
AUID- ORCID: 0000-0003-3981-8849
AD  - Center for Clinic Stem Cell Research, The Affiliated Drum Tower Hospital of 
      Nanjing University Medical School, Nanjing 210008, Jiangsu, People's Republic of 
      China.
FAU - Suo, Guangli
AU  - Suo G
AUID- ORCID: 0000-0003-4381-3864
AD  - CAS Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and 
      Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, Jiangsu, People's 
      Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211124
PL  - England
TA  - Biomed Mater
JT  - Biomedical materials (Bristol, England)
JID - 101285195
RN  - 0 (Biocompatible Materials)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 62031-54-3 (Fibroblast Growth Factors)
SB  - IM
MH  - *Biocompatible Materials
MH  - Extracellular Matrix/chemistry
MH  - Fibroblast Growth Factors
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - *Mesenchymal Stem Cells
MH  - Umbilical Cord
MH  - Vascular Endothelial Growth Factor A
MH  - Wound Healing
OTO - NOTNLM
OT  - ECM
OT  - angiogenesis
OT  - endothelial cells
OT  - hUCMSCs
EDAT- 2021/11/09 06:00
MHDA- 2022/04/01 06:00
CRDT- 2021/11/08 20:32
PHST- 2021/09/11 00:00 [received]
PHST- 2021/11/08 00:00 [accepted]
PHST- 2021/11/09 06:00 [pubmed]
PHST- 2022/04/01 06:00 [medline]
PHST- 2021/11/08 20:32 [entrez]
AID - 10.1088/1748-605X/ac37b0 [doi]
PST - epublish
SO  - Biomed Mater. 2021 Nov 24;17(1). doi: 10.1088/1748-605X/ac37b0.