PMID- 34749877
OWN - NLM
STAT- MEDLINE
DCOM- 20211110
LR  - 20211110
IS  - 1000-8020 (Print)
IS  - 1000-8020 (Linking)
VI  - 50
IP  - 5
DP  - 2021 Sep
TI  - [Effects of interleukin-17 antibody on polarization of macrophages in adipose 
      tissue of high-fat diet fed mice exposed to bisphenol A].
PG  - 814-820
LID - 10.19813/j.cnki.weishengyanjiu.2021.05.018 [doi]
AB  - OBJECTIVE: To investigate the effects of interleukin-17(IL-17) antibody on 
      polarization of adipose tissue macrophages(ATM) in mice fed with high-fat 
      diet(HFD) exposed to bisphenol A(BPA). METHODS: Four week-old male C57 BL/6 mice 
      were divided into control group, IgG group, IL-17 antibody group, 1000 nmol/L BPA 
      group, 1000 nmol/L BPA+IgG group, and 1000 nmol/L BPA+IL-17 antibody group 
      according to random number table method. Eight mice per group were fed with HFD 
      and BPA was exposed by drinking water. The IgG group and the 1000 nmol/L BPA+IgG 
      group were intraperitoneally injected with 100 mug IgG antibody once a week, and 
      the IL-17 group and the 1000 nmol/L BPA+IL-17 antibody group were 
      intraperitoneally injected with 100 mug IL-17 antibody once a week. After 16 
      weeks, the mice were sacrificed, and serum samples were collected for serum 
      separation. Leptin level was measured by enzyme-linked immunosorbent 
      assay(ELISA). The expression of IL-1beta, IL-6 and monocyte chemoattractant 
      protein-1(MCP-1)inflammatory cytokines were observed by immunohistochemistry(IHC) 
      of adipose tissue of epididymis. The activity of inducible nitric oxide 
      synthase(iNOS)and arginase-1(Arg-1)was measured by ELISA, and the proportion of 
      M1 and M2 ATMs was measured by flow cytometry(FCM). The expression of CD11 c and 
      CD206 mRNA was measured by qRT-PCR. RESULTS: Compared with the control group, 
      serum leptin, the expressions of inflammatory cytokines IL-6, IL-1beta and MCP-1 in 
      adipose tissue were increased in 1000 nmol/L BPA group, the proportion of M1 type 
      ATM was increased(22.000%+/-0.500% vs. 31.467%+/-0.379%), iNOS activity was 
      increased, CD11 c mRNA expression was increased, Arg-1 activity was decreased, 
      CD206 mRNA expression was decreased, the differences were statistically 
      significant(P<0.05), but the proportion of M2 type ATM was decreased 
      insignificantly(P>0.05). There was no significant change in IgG group. 
      Compared with 1000 nmol/L BPA group, IgG+1000 nmol/L BPA group had no significant 
      change. In 1000 nmol/L BPA+IL-17 antibody group, serum leptin was decreased, the 
      expressions of inflammatory factors IL-6, IL-1beta and MCP-1 in adipose tissue were 
      down-regulated, and the proportion of M1 type ATM was decreased(31.467%+/-0.379% 
      vs. 22.933%+/-0.153%), iNOS activity was decreased, CD11 c mRNA expression was 
      decreased, and the proportion of M2 type ATM was increased(4.847%+/-0.655% vs. 
      7.840%+/-0.555%), Arg-1 activity was enhanced, and CD206 mRNA expression was 
      up-regulated, the differences were statistically significant(P<0.05). 
      CONCLUSION: IL-17 antibody may reduce the secretion of ATM inflammatory factors 
      by inhibiting the polarization of ATM to M1 type, thus improving the inflammation 
      of adipose tissue in BPA-infected HFD-fed mice.
FAU - Zhou, Yi
AU  - Zhou Y
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Anhui Medical University, Hefei 230032, China.
FAU - Lu, Xiaotong
AU  - Lu X
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Anhui Medical University, Hefei 230032, China.
FAU - Luo, Shimeng
AU  - Luo S
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Anhui Medical University, Hefei 230032, China.
FAU - Li, Yingpei
AU  - Li Y
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Anhui Medical University, Hefei 230032, China.
FAU - Zhang, Yuheng
AU  - Zhang Y
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Anhui Medical University, Hefei 230032, China.
FAU - Li, Zuo
AU  - Li Z
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Anhui Medical University, Hefei 230032, China.
FAU - Zhu, Zhiyuan
AU  - Zhu Z
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Anhui Medical University, Hefei 230032, China.
FAU - Shen, Tong
AU  - Shen T
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Anhui Medical University, Hefei 230032, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Wei Sheng Yan Jiu
JT  - Wei sheng yan jiu = Journal of hygiene research
JID - 9426367
RN  - 0 (Benzhydryl Compounds)
RN  - 0 (Interleukin-17)
RN  - 0 (Phenols)
RN  - MLT3645I99 (bisphenol A)
SB  - IM
MH  - Adipose Tissue
MH  - Animals
MH  - Benzhydryl Compounds
MH  - *Diet, High-Fat/adverse effects
MH  - *Interleukin-17/genetics
MH  - Macrophages
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Phenols
OTO - NOTNLM
OT  - bisphenol A
OT  - inflammation
OT  - interleukin-17 antibody
OT  - macrophage
OT  - polarized
EDAT- 2021/11/10 06:00
MHDA- 2021/11/11 06:00
CRDT- 2021/11/09 05:46
PHST- 2021/11/09 05:46 [entrez]
PHST- 2021/11/10 06:00 [pubmed]
PHST- 2021/11/11 06:00 [medline]
AID - 10.19813/j.cnki.weishengyanjiu.2021.05.018 [doi]
PST - ppublish
SO  - Wei Sheng Yan Jiu. 2021 Sep;50(5):814-820. doi: 
      10.19813/j.cnki.weishengyanjiu.2021.05.018.