PMID- 3475135
OWN - NLM
STAT- MEDLINE
DCOM- 19870909
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 70
IP  - 2
DP  - 1987 Aug
TI  - Actin polymerization and its relationship to locomotion and chemokinetic response 
      in maturing human promyelocytic leukemia cells.
PG  - 363-7
AB  - We studied actin polymerization in the HL-60 human promyelocytic leukemia cell 
      line during induced myeloid maturation and its relationship to the rate of 
      locomotion (ROL). The percent G-actin (of total actin) was measured by DNAase I 
      inhibition, F-actin was determined by fluorescence-activated cell sorter (FACS) 
      analysis of nitrobenzoxadiazol (NBD)-phallacidin-stained cells, and ROL was 
      measured by computer-assisted analysis of the tracks of individual cells. 
      Uninduced HL-60 cells moved slowly (2.3 +/- 1.0 microns/min) and showed no change 
      in ROL or in the state of actin polymerization when stimulated by 
      formyl-methionyl-leucyl-phenylalanine (fMLP). Nonstimulated cells induced to 
      differentiate with dimethylformamide had no change in the degree of actin 
      polymerization but exhibited a mean (m) ROL similar to normal human 
      polymorphonuclear leukocytes (PMN) (8.6 +/- 1.4 micron/min [HL-60 cells] v 7.8 
      +/- 1.8 microns/min [PMN]. When induced HL-60 cells were stimulated with fMLP, 
      actin polymerization occurred. The F-actin content increased, as determined by 
      FACS analysis of NBD-phallacidin-stained cells, and the percentage of G-actin 
      decreased, as determined by a 24.5% decrease in DNAase I inhibitory activity. 
      However, induced HL-60 cells stimulated with fMLP did not increase their mROL. 
      These studies show that, unlike normal human PMN, chemotactic peptides can cause 
      an intracellular biochemical change that is not associated with a chemokinetic 
      response in induced HL-60 cells. The HL-60 cell line may be a useful model to 
      study the development of chemotactic peptide-mediated actin polymerization during 
      myeloid cell maturation.
FAU - Meyer, W H
AU  - Meyer WH
FAU - Howard, T H
AU  - Howard TH
LA  - eng
GR  - GM 29477/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Actins)
RN  - 0 (Polymers)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
SB  - IM
MH  - Actins/*metabolism
MH  - Cell Line
MH  - Cell Movement
MH  - Cell Survival
MH  - *Chemotaxis
MH  - Humans
MH  - Leukemia, Myeloid, Acute/metabolism/*pathology/physiopathology
MH  - N-Formylmethionine Leucyl-Phenylalanine
MH  - Polymers/*biosynthesis
EDAT- 1987/08/01 00:00
MHDA- 1987/08/01 00:01
CRDT- 1987/08/01 00:00
PHST- 1987/08/01 00:00 [pubmed]
PHST- 1987/08/01 00:01 [medline]
PHST- 1987/08/01 00:00 [entrez]
AID - S0006-4971(20)78422-3 [pii]
PST - ppublish
SO  - Blood. 1987 Aug;70(2):363-7.