PMID- 34754960
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211111
IS  - 2405-6383 (Electronic)
IS  - 2405-6545 (Print)
IS  - 2405-6383 (Linking)
VI  - 7
IP  - 4
DP  - 2021 Dec
TI  - Rumen-protected glucose supplementation in transition dairy cows shifts 
      fermentation patterns and enhances mucosal immunity.
PG  - 1182-1188
LID - 10.1016/j.aninu.2021.08.002 [doi]
AB  - Manipulation of perinatal diets, such as supplementing feed with rumen-protected 
      glucose (RPG), has been positively regarded as a strategy to improve milking 
      performance. This study was conducted to assess the effects of RPG on the 
      fermentation profiles, resident microbiota and mucosal immunity in the cecum. Ten 
      Holstein dairy cows were randomly assigned to either a 25 g/kg RPG diet (DM 
      basis) or a 11 g/kg coating fat diet (control, CON). Compared with the CON group, 
      the acetate-to-propionate ratio was lower in the RPG group. Gene expression 
      analysis indicated that RPG supplementation tended to upregulate the expression 
      of Na(+)/H(+) hydrogen exchanger 3 (NHE3) (P = 0.076). RPG supplementation 
      downregulated the expression of genes involved in self-rehabilitation such as 
      matrix metalloproteinase 1 (MMP1), MMP3, MMP9 and MMP13. Additionally, the mRNA 
      expression of genes involved in immunity including Toll-like receptors (TLR4, 
      TLR6 and TLR7) and proinflammatory cytokines (immune interferon gamma [IFNG] and 
      interleukins interleukin 17A [IL17F], IL17A, IL22), was downregulated by RPG 
      supplementation. Nonetheless, no differences existed in the bacterial copy number 
      and beta diversity between the 2 groups. Overall, supplementation with RPG would 
      probably cause a shift towards propionate production in the cecal digesta, and 
      promote the immune homeostasis of the cecal mucosa in transition dairy cows. Our 
      results extended the basic understanding of RPG supplementation and utilization 
      in transition dairy cows in terms of host microbe interplay in the cecum.
CI  - (c) 2021 Chinese Association of Animal Science and Veterinary Medicine. Publishing 
      services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.
FAU - Zhang, Xiaoli
AU  - Zhang X
AD  - CAS Key Laboratory of Agro-ecological Processes in Subtropical Region, National 
      Engineering Laboratory for Pollution Control and Waste Utilization in Livestock 
      and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional 
      Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese 
      Academy of Sciences, Changsha, Hunan, 410125, China.
AD  - University of the Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Li, Xiaopeng
AU  - Li X
AD  - CAS Key Laboratory of Agro-ecological Processes in Subtropical Region, National 
      Engineering Laboratory for Pollution Control and Waste Utilization in Livestock 
      and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional 
      Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese 
      Academy of Sciences, Changsha, Hunan, 410125, China.
FAU - Wu, Jian
AU  - Wu J
AD  - CAS Key Laboratory of Agro-ecological Processes in Subtropical Region, National 
      Engineering Laboratory for Pollution Control and Waste Utilization in Livestock 
      and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional 
      Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese 
      Academy of Sciences, Changsha, Hunan, 410125, China.
AD  - University of the Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Jiao, Jinzhen
AU  - Jiao J
AD  - CAS Key Laboratory of Agro-ecological Processes in Subtropical Region, National 
      Engineering Laboratory for Pollution Control and Waste Utilization in Livestock 
      and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional 
      Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese 
      Academy of Sciences, Changsha, Hunan, 410125, China.
FAU - He, Zhixiong
AU  - He Z
AD  - CAS Key Laboratory of Agro-ecological Processes in Subtropical Region, National 
      Engineering Laboratory for Pollution Control and Waste Utilization in Livestock 
      and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional 
      Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese 
      Academy of Sciences, Changsha, Hunan, 410125, China.
FAU - Tan, Zhiliang
AU  - Tan Z
AD  - CAS Key Laboratory of Agro-ecological Processes in Subtropical Region, National 
      Engineering Laboratory for Pollution Control and Waste Utilization in Livestock 
      and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional 
      Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese 
      Academy of Sciences, Changsha, Hunan, 410125, China.
FAU - Han, Xuefeng
AU  - Han X
AD  - CAS Key Laboratory of Agro-ecological Processes in Subtropical Region, National 
      Engineering Laboratory for Pollution Control and Waste Utilization in Livestock 
      and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional 
      Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese 
      Academy of Sciences, Changsha, Hunan, 410125, China.
LA  - eng
PT  - Journal Article
DEP - 20211002
PL  - China
TA  - Anim Nutr
JT  - Animal nutrition (Zhongguo xu mu shou yi xue hui)
JID - 101717655
PMC - PMC8556486
OTO - NOTNLM
OT  - Cecum
OT  - Fatty acid metabolism
OT  - Fermentation
OT  - Microbiota
OT  - Rumen-protected glucose
COIS- We declare that we have no financial and personal relationships with other people 
      or organizations that can inappropriately influence our work, and there is no 
      professional or other personal interest of any nature or kind in any product, 
      service and/or company that could be construed as influencing the content of this 
      paper.
EDAT- 2021/11/11 06:00
MHDA- 2021/11/11 06:01
PMCR- 2021/10/02
CRDT- 2021/11/10 06:47
PHST- 2020/10/15 00:00 [received]
PHST- 2021/07/23 00:00 [revised]
PHST- 2021/08/03 00:00 [accepted]
PHST- 2021/11/10 06:47 [entrez]
PHST- 2021/11/11 06:00 [pubmed]
PHST- 2021/11/11 06:01 [medline]
PHST- 2021/10/02 00:00 [pmc-release]
AID - S2405-6545(21)00166-9 [pii]
AID - 10.1016/j.aninu.2021.08.002 [doi]
PST - ppublish
SO  - Anim Nutr. 2021 Dec;7(4):1182-1188. doi: 10.1016/j.aninu.2021.08.002. Epub 2021 
      Oct 2.